1. Preferential associations between co-regulated genes reveal a transcriptional interactome in erythroid cells.
- Author
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Schoenfelder, Stefan, Sexton, Tom, Chakalova, Lyubomira, Cope, Nathan F., Horton, Alice, Andrews, Simon, Kurukuti, Sreenivasulu, Mitchell, Jennifer A., Umlauf, David, Dimitrova, Daniela S., Eskiw, Christopher H., Yanquan Luo, Chia-Lin Wei, Yijun Ruan, Bieker, James J., and Fraser, Peter
- Subjects
GENOMES ,GENE expression ,GLOBIN genes ,TRANSCRIPTION factors ,GENETIC regulation - Abstract
The discovery of interchromosomal interactions in higher eukaryotes points to a functional interplay between genome architecture and gene expression, challenging the view of transcription as a one-dimensional process. However, the extent of interchromosomal interactions and the underlying mechanisms are unknown. Here we present the first genome-wide analysis of transcriptional interactions using the mouse globin genes in erythroid tissues. Our results show that the active globin genes associate with hundreds of other transcribed genes, revealing extensive and preferential intra- and interchromosomal transcription interactomes. We show that the transcription factor Klf1 mediates preferential co-associations of Klf1-regulated genes at a limited number of specialized transcription factories. Our results establish a new gene expression paradigm, implying that active co-regulated genes and their regulatory factors cooperate to create specialized nuclear hot spots optimized for efficient and coordinated transcriptional control. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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