1. Production of biologically active human factor IX-Fc fusion protein in the milk of transgenic mice.
- Author
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Yan, Hong, Gong, Xiuli, Xu, Miao, Guo, Xinbing, Chen, Yanwen, Xue, Yan, Zeng, Yitao, and Zeng, Fanyi
- Subjects
TRANSGENIC mice ,MILK proteins ,CHIMERIC proteins ,TRANSGENIC animals ,MAMMARY glands ,HEMOPHILIA treatment ,BLOOD coagulation factor IX - Abstract
Objective: To investigate the feasibility of producing human IgG1 Fc fragment fused factor IX (FIX-Fc) in the milk of transgenic animals, for an alternative possible solution to the unmet need of FIX-Fc products for hemophilia B treatment. Results: Six founder lines of transgenic mice harboring FIX-Fc cassette designed to be expressed specifically in the mammary gland were generated. FIX-Fc protein was secreted into the milk of transgenic mice with preserved biological activity (with the highest value of 6.2 IU/mL), similar to that of the non-fused FIX transgenic milk. RT-PCR and immunofluorescence analysis confirmed that FIX-Fc was specifically expressed in the mammary gland. The blood FIX clotting activities were unchanged, and no apparent health defects were observed in the transgenic mice. Moreover, the stability of FIX protein in milk was increased by the Fc fusion. Conclusions: It is feasible to produce biologically functional FIX-Fc in the mammary gland of transgenic mice. Our preliminary results provide a foundation for the potential scale-up production of FIX-Fc in the milk of dairy animals. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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