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Enhancing the lipid production of oleaginous yeasts is conducive to cutting the cost of feedstock for biodiesel. To increase the lipid productivity of Rhodotorula sp. U13N3, genes involving lipid degradation were knocked out and fermentation conditions were investigated. Results of transcription analysis demonstrated that genes encoding the ATG15-like lipase (ATG15) and peroxisomal acyl-CoA oxidase (ACOX2) were upregulated significantly at the lipogenesis stage. When ATG15 and ACOX2 were knocked out separately from the genome by the CRISPR/Cas9 method, both ΔATG15 and ΔACOX2 mutants showed better lipid production ability than the parent strain. Flow cytometry and confocal microscopic analyses indicated that simultaneous the knockout of ATG15 and ACOX2 did not impact the cell viability, whereas the lipid production was enhanced markedly as the lipid yield increased by 67.03% in shake flasks. Afterward, the ΔATG15ΔACOX2 transformant (TO2) was cultivated in shake flasks in the fed-batch mode; the highest biomass and lipid yield reached 45.76 g/L and 27.14 g/L at 216 h, respectively. Better performance was achieved when TO2 was cultivated in the 1-L bioreactor. At the end of fermentation (180 h), lipid content, yield, yield coefficient, and productivity reached 65.53%, 27.35 g/L, 0.277 g/g glycerol, and 0.152 g/L/h, respectively. These values were at the high level in comparison with Rhodotorula strains cultivated in glycerol media. Besides, fermentation modes did not affect the fatty acid composition of TO2 significantly. In conclusion, blocking the lipid degradation was an applicable strategy to increase the lipid production of Rhodotorula strains without compromising their cell viability. Key points: • ATG15-like lipase and acyl-CoA oxidase (ACOX2) participated in lipid degradation. • Knockout of ATG15 and ACOX2 increased lipid productivity, and lipid yield coefficient. • Cell viability maintained at high level in the knockout mutants during fermentation. [ABSTRACT FROM AUTHOR]

Electro-optic (EO) modulator plays a very critical role in the optical communication systems. Here, we report a stimulated thin-film lithium niobate (LN) modulator with a half-wave voltage-length product of 1.8 V·cm, which can successfully achieve modulation and demodulation of a 1 GHz sinusoidal signal with an amplitude of 5 V in experiment. The optical loss caused by metal electrodes is reduced by optimizing the waveguide structure and depositing silica onto the waveguide, and group-velocity matching and characteristic impedance matching are achieved by optimizing the buffer silica layer and the electrode structure for larger bandwidth, which simultaneously improves the modulation efficiency and bandwidth performance. Our work demonstrated here paves a foundation for integrated photonics. [ABSTRACT FROM AUTHOR]

Requirement of clean energy sources urges us to find substitutes for fossil fuels. Microorganisms provide an option to produce feedstock for biofuel production by utilizing inexpensive, renewable biomass. Rhodotorula toruloides (Rhodosporidium toruloides), a non-conventional oleaginous yeast, can accumulate intracellular lipids (single cell oil, SCO) more than 70% of its cell dry weight. At present, the SCO-based biodiesel is not a price-competitive fuel to the petroleum diesel. Many efforts are made to cut the cost of SCO by strengthening the performance of genetically modified R. toruloides strains and by valorization of low-cost biomass, including crude glycerol, lignocellulosic hydrolysates, food and agro waste, wastewater, and volatile fatty acids. Besides, optimization of fermentation and SCO recovery processes are carefully studied as well. Recently, new R. toruloides strains are developed via metabolic engineering and synthetic biology methods to produce value-added chemicals, such as sesquiterpenes, fatty acid esters, fatty alcohols, carotenoids, and building block chemicals. This review summarizes recent advances in the main aspects of R. toruloides studies, namely, construction of strains with new traits, valorization of low-cost biomass, process detection and optimization, and product recovery. In general, R. toruloides is a promising microbial cell factory for production of biochemicals. [ABSTRACT FROM AUTHOR]

Activating the defense response of plants by elicitors provides a promising method for biocontrol of pathogens. The homogeneous octaose (RiOc) which was depolymerized from the succinoglycan Riclin was investigated as a novel elicitor to activate the immune system of potato (Solanum tuberosum L.). After foliar spray, RiOc quickly induced accumulation of reactive oxygen species in potato leaves in a dose-dependent manner. Transcriptomic analysis revealed that 2712 out of 30,863 genes were differentially expressed at the early stage (24 h), while 367 of them were changed later (72 h). Results from the transcriptome and quantitative RT-PCR suggested that RiOc was probably perceived by the receptor LYK3 and it activated the MKK2/3/9/-MPK6/7 signaling cascade and promoted the salicylic acid-mediated defense response. Meanwhile, RiOc changed the metabolome profile of potato leaves over time as demonstrated by the 1H NMR-based metabolomic analysis. Homeostasis of amino acids was affected at the early stage while the secondary metabolism was strengthened later. More importantly, RiOc significantly reduced the severity of potato leaf lesions caused by the late blight pathogen Phytophthora infestans. In conclusion, RiOc effectively improved the resistance of potato to P. infestans by eliciting the salicylic acid-mediated defense response. RiOc becomes a promising carbohydrate-based elicitor for biocontrol of plant pathogens. Key points: • Homogeneous Riclin octaose was a novel elicitor for biocontrol of plant pathogens. • Riclin octaose primed the salicylic acid-mediated defense response of potato plants. • Riclin octaose changed the metabolome profile of potato leaves over time. [ABSTRACT FROM AUTHOR]

Lipids produced by oleaginous microorganisms enrich the supply of feedstock for bio-fuel. In this study, a mutant (Mut) obtained by UV-nitrosoguanidine mutagenesis turned its colony color from orange–red to light-yellow and showed higher lipid productivity at 37 °C than the original strain Rhodotorula sp. U13N3 (Rht) in the glycerol medium. The metabolic changes between Mut and Rht in batch fermentation were investigated by transcriptomic and metabolomic profiling at the biomass accumulation (30 h) and lipid production (96 h) stages. The average base number in each strain was 5.80 × 109 ± 0.38 × 109 bp (mean ± SD) with 62.43% ± 0.13% GC ratio, and 7499 unigenes were assembled after Illumina sequencing. Moreover, 33 metabolites were quantified by 1H NMR-based profiling. The multi-omics results demonstrated that Mut showed increased glycerol transport and utilization capabilities especially at the first stage (30 h). Then the carbon flux shifted from the TCA cycle to lipid production (96 h). The increased lipid productivity of Mut was partially attributed to the down-regulation of mannitol biosynthesis. However, the mechanism for color change was elusive. At 96 h, the low level of cytosol glycerol probably restricted the lipid production. As a result, supplementation of glycerol in fed-batch fermentation remarkably improved the biomass, lipid production, and lipid content to 34.60 g/L, 25.72 g/L, and 74.3% (w/w dcw), respectively. The cell morphology implied that excessively prolonging the fermentation time was detrimental to the final lipid yield due to cell breakage. In conclusion, the Rhodotorula mutant provided a candidate strain for lipid production with glycerol as the carbon source. [ABSTRACT FROM AUTHOR]

Levan, a type of β (2→6)-linked fructan, is a promising biopolymer with distinct properties and extensive applications in the fields of food, pharmaceutical, cosmetics, etc. However, the commercial availability of levan is still limited due to the relatively high production costs. Here, a new Paenibacillus sp. strain FP01 was isolated and identified as an efficient fructan producer with high yield (around 89.5 g/L fructan was obtained under 180 g/L sucrose) and conversation rate (49.7%). The fructan named Plev was structurally characterized as a linear levan-type fructan with a molecular mass of 3.11 × 106 Da. Aqueous solutions of Plev exhibited a non-Newtonian behavior at concentrations 3–5%. Heating and chilling had no obvious effects on apparent viscosities of Plev solutions. Plev also had good rheological stabilities toward pH (3–11) and metal salts (Na+, K+, Ca2+, Mg2+). Microbiome and metabolome analysis showed that Plev intervention increased the abundance of beneficial bacteria and elevated the levels of short-chain fatty acids (SCFAs) in feces of mice. Taken together, Plev could be considered a potential thickener and prebiotic supplement in food industry. Key points • Paenibacillus sp. strain FP01 was identified as a high-efficient levan producer. • The levan Plev from FP01 exhibited good rheological properties and stabilities. • The in vivo prebiotic activities of linear levan were revealed. [ABSTRACT FROM AUTHOR]

Aims/hypothesis: Plasma 5′-AMP (pAMP) is elevated in mouse models of type 2 diabetes. However, the metabolic regulatory role of adenine nucleotides in type 2 diabetes remains unclear. Methods: Adenine nucleotides and their metabolites in plasma and liver were examined by HPLC. 1H NMR-based metabolomics analysis was performed to explore the changes of metabolites in mouse models of type 2 diabetes. Na+/K+ ATPase and Na+/H+ exchanger activity were measured in response to adenine nucleotide metabolites. Human recombinant protein tyrosine phosphatase 1B (PTP1B) was used for enzyme kinetic assays. Protein binding assays were performed with microscale thermophoresis. The intracellular pH of hepatocyte AML12 cell lines was measured using the BCECF-AM method. We also analysed pAMP levels in participants with type 2 diabetes. Results: Elevation of pAMP was a universal phenomenon in all mouse models of type 2 diabetes including db/db vs lean mice (13.9 ± 2.3 μmol/l vs 3.7 ± 0.9 μmol/l; p < 0.01), ob/ob vs lean mice (9.1 ± 2.0 μmol/l vs 3.9 ± 1.2 μmol/l; p < 0.01) and high-fat diet/streptozotocin-induced vs wild-type mice (6.6 ± 1.5 μmol/l vs 4.1 ± 0.9 μmol/l; p < 0.05); this elevation was required for the occurrence of hyperglycaemia in obese mice. 1H NMR-based metabolomics study following HPLC analysis revealed that the metabolite profile in wild-type mice treated with 5′-AMP was similar to that in db/db diabetic mice, especially the accumulation of a large quantity of ATP and its metabolites. The glucose-lowering drug metformin reduced the severity of hyperglycaemia both in 5′-AMP-induced wild-type mice and db/db mice. Metformin decreased the accumulation of liver ATP but not its metabolites in these hyperglycaemic mice. ATP and metformin reciprocally change cellular pH homeostasis in liver, causing opposite shifts in liver activity of PTP1B, a key negative regulator of insulin signalling. Furthermore, pAMP levels were also elevated in individuals with type 2 diabetes (45.2 ± 22.7 nmol/l vs 3.1 ± 1.9 nmol/l; p < 0.01). Conclusions/interpretation: These results reveal an emerging role for adenine nucleotide in the regulation of hyperglycaemia and provide a potential therapeutic target in obesity and type 2 diabetes. [ABSTRACT FROM AUTHOR]

Purpose: Methotrexate (MTX) is a widely used cancer chemotherapy agent. The efficacy of MTX is often limited by serious side effects, such as intestinal mucositis. The aim of this study was to evaluate the protective effect of water-soluble β-glucan salecan on MTX-induced intestinal toxicity in mice.Methods: Intestinal mucositis was induced in C57BL/6 mice by intraperitoneal injection of MTX for two consecutive days. Mice were orally administrated with saline or salecan for 6 days before MTX injection and continued to the end of the study. Several histological and biochemical parameters were measured in the jejunum.Results: Orally administration of salecan improved the severity of intestinal mucositis in a dose-dependent manner, as evidenced by the well-maintained mucosal architecture and body weight in salecan-treated groups. Salecan treatment inhibited MTX-induced oxidative stress and effectively scavenged free radicals both in vitro and in vivo. Metabolomics analysis revealed that salecan treatment reversed the intestinal metabolic profiling changes in mice with MTX-induced mucositis. Salecan treatment modulated the innate immunity through the regulation of TLR and Dectin1 expression in the jejunum, thus protecting mice from MTX-induced intestinal damage.Conclusions: Salecan has potential advantages in the treatment of MTX-induced intestinal mucositis, and its protective effect is mainly attributed to its antioxidant and immunomodulatory properties. [ABSTRACT FROM AUTHOR]

Natural polymeric flocculant shows effectiveness in wastewater treatment without increasing the environmental burden. The extracellular substance produced by Paenibacillus mucilaginosus WL412 was identified as an anionic polysaccharide composed of five types of monosaccharides, namely, D-mannose, D-glucuronic acid, D-glucose, D-galactose, and L-fucose with the molar ratio of 2.8:1.2:2.0:1.8:0.8. The purified polysaccharide, POS412, presented high efficiency in flocculating coal washing wastewater and kaolin suspension without the assistance of inorganic coagulants. Addition of POS412 resulted in the polymer bridging phenomenon in suspensions, which was observed by means of scanning electron microscopic imaging, size grading, and ζ-potential analyses. More importantly, POS412 exhibited satisfactory stability after storage in various conditions. The flocculation rate was more than 91% for coal washing wastewater when POS412 was stored for 264 h in the wide range of pH (3-11) and temperature (20-50 °C) before use. Results indicate that POS412 is a competent bioflocculant for wastewater treatment. [ABSTRACT FROM AUTHOR]

Salecan, a soluble β-1,3- d-glucan produced by a salt-tolerant strain Agrobacterium sp. ZX09, has been the subject of considerable interest in recent years because of its multiple bioactivities and unusual rheological properties in solution. In this study, both succinyl and pyruvyl substituent groups on salecan were identified by an enzymatic hydrolysis following nuclear magnetic resonance (NMR), HPLC, and MS analysis. The putative succinyltransferase gene ( sleA) and pyruvyltransferase gene ( sleV) were determined and cloned. Disruption of the sleA gene resulted in the absence of succinyl substituent groups on salecan. This defect could be complemented by expressing the sleA cloned in a plasmid. Thus, the sleA and sleV genes located in a 19.6-kb gene cluster may be involved in salecan biosynthesis. Despite the lack of succinyl substituents, the molecular mass of salecan generated by the sleA mutant did not substantially differ from that generated by the wild-type strain. Loss of succinyl substituents on salecan changed its rheological characteristics, especially a decrease in intrinsic viscosity. [ABSTRACT FROM AUTHOR]

Bacterial glucans have aroused increasing interest in commercial applications in the food and pharmaceutical sectors. A number of bacterial glucans have been reported over recent decades, and their structure, production, and functional properties have been extensively studied. In this paper, we review recent researches on bacterial glucans, with emphasis on the production, physical and chemical properties, and the new developments in food, biomedical, pharmaceutical, and other industrial applications. [ABSTRACT FROM AUTHOR]

A new β-1,3-1,4-glucanase gene ( PlicA) was cloned from Paenibacillus sp. S09. The ORF contained 717 bp coding for a 238 amino acid protein. PlicA, expressed in Escherichia coli and purified by Ni-affinity chromatography, had optimum activity at 55 °C and pH 6.2. The specific activity toward barley β-glucan reached 7,055 U/mg. K and V values with barley β-glucan were 3.7 mg/ml and 3.3 × 10 μmol/min mg, respectively. The enzyme exhibited acid- and alkali-tolerance with more than 80 % activity remaining after incubation for 4 h at pH 3.5-12. PlicA was salt-tolerant (>90 % activity retained in 4 M NaCl at 25 °C for 24 h) and salt-activated: activity rising 1.5-fold in 0.5 M NaCl. The thermostability was improved by NaCl and CaCl. This is the first report of an acid-, alkali- and salt-tolerant bacterial β-1,3-1,4-glucanase with high catalytic efficiency. [ABSTRACT FROM AUTHOR]

Current efforts in metamaterials research focus on attaining dynamic functionalities such as tunability, switching and modulation of electromagnetic waves. To this end, various approaches have emerged, including embedded varactors, phase-change media, the use of liquid crystals, electrical modulation with graphene and superconductors, and carrier injection or depletion in semiconductor substrates. However, tuning, switching and modulating metamaterial properties in the visible and near-infrared range remain major technological challenges: indeed, the existing microelectromechanical solutions used for the sub-terahertz and terahertz regimes cannot be shrunk by two to three orders of magnitude to enter the optical spectral range. Here, we develop a new type of metamaterial operating in the optical part of the spectrum that is three orders of magnitude faster than previously reported electrically reconfigurable metamaterials. The metamaterial is actuated by electrostatic forces arising from the application of only a few volts to its nanoscale building blocks-the plasmonic metamolecules-that are supported by pairs of parallel strings cut from a flexible silicon nitride membrane of nanoscale thickness. These strings, of picogram mass, can be driven synchronously to megahertz frequencies to electromechanically reconfigure the metamolecules and dramatically change the transmission and reflection spectra of the metamaterial. The metamaterial's colossal electro-optical response (on the order of 10−5-10−6 m V−1) allows for either fast continuous tuning of its optical properties (up to 8% optical signal modulation at up to megahertz rates) or high-contrast irreversible switching in a device only 100 nm thick, without the need for external polarizers and analysers. [ABSTRACT FROM AUTHOR]

Graphene plasmons are rapidly emerging as a versatile platform for manipulating light at the deep subwavelength scale. Here we show numerically that strong optical near-field forces can be generated under the illumination of mid-IR light when dielectric nanoparticles are located in the vicinity of a nanostructured graphene film. These near-field forces are attributed to the excitation of the graphene's plasmonic mode. The optical forces can generate an efficient optical trapping potential for a 10-nm-diameter dielectric particle when the light intensity is only about about 4.4 mW/μm2 and provide possibilities for a new type of plasmonic nano-tweezers. Graphene plasmonic tweezers can be potentially exploited for optical manipulation of nanometric biomolecules and particles. Moreover, the optical trapping/tweezing can be combined with biosensing and provide a versatile platform for studing biology and chemistry with mid-IR light. [ABSTRACT FROM AUTHOR]

This paper presents an comprehensive study of light trapping and absorption enhancement in graphene through metallic plasmonic structures and shows a strategy to realize both ultrabroadband and strong absorption enhancement. Three different plasmonic absorber designs are investigated by numerical simulations. The excitation of localized plasmons in the metallic structures significantly enhances the interactions between graphene and light at the resonances. By employing a splitted cross design for plasmonic resonant antennas and integrating two types of sub-antennas with different sizes, more than 30% of optical absorption in monolayer graphene is realized in a ultrabroad spectral range from 780 to 1760 nm. This enhancement functionality can be translated to any wavelength band from ultraviolet to terahertz ranges by modifying the geometric design of the plasmonic structure and can be applied for other two dimensional materials and their heterogeneous structures. It may significantly improve the efficiency of optical devices such as broadband photodetectors and solar cells based on graphene and other two-dimensional materials. [ABSTRACT FROM AUTHOR]

Phase-contrast x-ray imaging using an x-ray interferometer has great potential to reveal the structures inside soft tissues, because the sensitivity of this method to hydrogen, carbon, nitrogen, and oxygen is about 1000 times higher than that of the absorption-contrast x-ray method. In this study, we used phase-contrast X-ray CT to investigate human resected esophagogastric junction. This technology revealed the three-layer structure of the esophagogastric junction wall-mucous, submucosa and muscular layers. The mucous and muscular layers were clearly separated by a loose submucosa layer with a honeycomb appearance. The shape of the mucous and muscular layers was intact. The boundary between the mucous and submucosa layers was distinct, as was the border of the muscular and submucosa layers. The surface of the esophagogastric junction was displayed clearly through 3D reconstruction. The technology might be helpful in the diagnosis of esophagogastric junction lesion, especially for the early adenocarcinoma. [ABSTRACT FROM AUTHOR]

A correction to the article "An electromechanically reconfigurable plasmonic metamaterial operating in the near-infrared" that was published in the March 17, 2013 issue is presented.