1. Efficient targeted mutagenesis in potato by the CRISPR/Cas9 system.
- Author
-
Wang, Shaohui, Zhang, Shuaibin, Wang, Wanxing, Xiong, Xingyao, Meng, Fanrong, and Cui, Xia
- Subjects
- *
CRISPRS , *ENDONUCLEASES , *PLANT genomes , *MOLECULAR cloning , *PLANT RNA , *NUCLEIC acid isolation methods , *POLYMERASE chain reaction ,POTATO genetics - Abstract
The article discusses a study which described the production of gene knockouts in potato via the clustered regularly interspaced short palindromic repeats/associated protein 9 nuclease (CRISPR/CAS9) system. Particular focus is given to the growing role of the CRISPR/CAS9 system in plant genome editing, as well as the importance of potato in global food security. Also discussed are cloning of a native promoter for potato RNA, DNA extraction and polymerase chain reaction on transgenic plants.
- Published
- 2015
- Full Text
- View/download PDF