1. The Phosphatome of Medicinal and Edible Fungus <italic>Wolfiporia cocos</italic>.
- Author
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Zhu, Wenjun, Wei, Wei, Zhang, Shaopeng, Zheng, Yonglian, Chen, Ping, and Xu, Xiaowen
- Subjects
EDIBLE fungi ,CHINESE medicine ,SCLEROTIUM (Mycelium) ,PHOSPHOPROTEIN phosphatases ,CELL cycle ,CELLULAR signal transduction - Abstract
Wolfiporia cocos is an important medicinal and edible fungus that grows in association with pine trees, and its dried sclerotium has been used as a traditional medicine in China for centuries. However, the commercial production ofW. cocos sclerotia is currently limited by shortages in pine wood resources. Since protein phosphatases (PPs) play significant roles in growth, signal transduction, development, metabolism, sexual reproduction, cell cycle, and environmental stress responses in fungi, the phosphatome ofW. cocos was analyzed in this study by identifying PP genes, studying transcript profiles and assigning PPs to orthologous groups. Fifty-four putative PP genes were putatively identified inW. cocos genome based on homologous sequences searching using BLASTx program against theSaccharomyces cerevisiae ,Fusarium graminearum , andSclerotinia sclerotiorum databases. Based on known and presumed functions of orthologues of these PP genes found in other fungi, the putative roles of theseW. cocos PPs in colonization, hyphal growth, sclerotial formation, secondary metabolism, and stress tolerance to environment were discussed in this study. And the level of transcripts from PP genes in the mycelium and sclerotium stages was also analyzed by qRT-PCR. Our study firstly identified and functional discussed the phosphatome in the medicinal and edible fungusW. cocos . The data from our study contribute to a better understanding of PPs potential roles in various cellar processes ofW. cocos , and systematically provide comprehensive and novel insights intoW. cocos economically important traits that could be extended to other fungi. [ABSTRACT FROM AUTHOR]- Published
- 2018
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