1. XAI-enabled neural network analysis of metabolite spatial distributions.
- Author
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Ma, Wenwu, Luo, Lanfang, Liang, Kun, Liu, Taoyan, Su, Jiali, Wang, Yuefan, Li, Jun, Zhou, S. Kevin, and Shyh-Chang, Ng
- Subjects
ARTIFICIAL neural networks ,CONVOLUTIONAL neural networks ,DEEP learning ,ARTIFICIAL intelligence ,MUSCLE aging ,MACHINE learning - Abstract
We used deep neural networks to process the mass spectrometry imaging (MSI) data of mouse muscle (young vs aged) and human cancer (tumor vs normal adjacent) tissues, with the aim of using explainable artificial intelligence (XAI) methods to rapidly identify biomarkers that can distinguish different classes of tissues, from several thousands of metabolite features. We also modified classic neural network architectures to construct a deep convolutional neural network that is more suitable for processing high-dimensional MSI data directly, instead of using dimension reduction techniques, and compared it to seven other machine learning analysis methods' performance in classification accuracy. After ascertaining the superiority of Channel-ResNet10, we used a novel channel selection–based XAI method to identify the key metabolite features that were responsible for its learning accuracy. These key metabolite biomarkers were then processed using MetaboAnalyst for pathway enrichment mapping. We found that Channel-ResNet10 was superior to seven other machine learning methods for MSI analysis, reaching > 98% accuracy in muscle aging and colorectal cancer datasets. We also used a novel channel selection–based XAI method to find that in young and aged muscle tissues, the differentially distributed metabolite biomarkers were especially enriched in the propanoate metabolism pathway, suggesting it as a novel target pathway for anti-aging therapy. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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