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The effect that the fructans of Cichorium intybus and Agave salmiana have on health, as well as on the growth of some Lactobacillus species, has been demonstrated. The aim of this work was to evaluate the effect of linear and branched fructans on the growth of seven strains and some probiotic characteristics. The molecular identification of seven strains was performed. Moreover, the growth, resistance to antibiotics and simulated gastrointestinal conditions were also evaluated when these microorganisms were grown in a culture medium containing agave and chicory fructans. The strains were identified as Lactiplantibacillus plantarum, Lactiplantibacillus pentosus, Lactiplantibacillus fabifermentans and Lactiplantibacillus paraplantarum. The results suggest that the seven Lactobacillus strains were able to grow using agave (branched) and chicory (linear) fructans. The linear and branched fructans statistically influenced the kinetic parameters. The specific growth rate varied between 0.270 and 0.573 h−1 and the generation time between 1.21 and 2.45 h for all strains and culture media. All strains showed a growth of 9 Log CFU/mL in all the culture media. Production of lactic, acetic, propionic, butyric, formic and succinic acid was influenced by linear and branched fructans (p < 0.05). All the strains survived simulated gastrointestinal conditions greater than 83%. The resistance of Lactobacillus against ciprofloxacin and rifaximin was significantly affected by linear and branched fructans, but survival to gastrointestinal conditions was not affected by the type of substrate. These results highlight the use of the seven strains, which have probiotic potential; therefore, these could be applied in several biotechnological products. [ABSTRACT FROM AUTHOR]

Most foods with probiotics claims are associated to dairy products, whose consumption is restricted to part of the population, creating a favorable scenario for the development of probiotic foods in alternative matrices. However, the development of probiotic foods in non-dairy matrices is still a technological challenge, since the foods intrinsic parameters can cause injuries to microorganisms. An alternative to protect the microbial cells in adverse environments involves encapsulation. Therefore, the objective of this study was to evaluate the influence of alginate-jaboticaba peel blend in the improvement of encapsulation efficiency, viability maintenance, and cell survival of Bifidobacterium BB12® under simulated gastrointestinal digestion and after incorporating in traditional jaboticaba jam. The particles were obtained by ion gelling technique using alginate with or without powdered jaboticaba peel. The addition of jaboticaba peel in particles improved encapsulation efficiency (> 90%) and resulted in higher cell survival in simulated gastrointestinal digestion. During storage in jam, the loss in cell viability was approximately constant: c.a. 0.5 log CFU/g/day for encapsulated cells and c.a. 1.0 log CFU/g/day for free cells. These results suggest that use of alginate and powdered jaboticaba peel blend is a promising approach to protect Bifidobacterium BB12® against adverse environments, such as non-dairy food matrices. Key points: • Powdered jaboticaba peel increased the encapsulation efficiency in alginate particles. • Encapsulation improved cell survival under adverse conditions. • Useful approach for the development of non-conventional probiotic products. Graphical abstract [ABSTRACT FROM AUTHOR]

Phytate is a potent inhibitor of mineral absorption in humans occurring in plant-based food. Application of lactobacilli that produce phytate-degrading enzymes (phytases) to reduce phytate is an interesting yet a not much explored sector of research. Therefore, phytate dephosphorylation by Lactobacillus plantarum MTCC 1325 was evaluated. Cells at stationary phase showed phytase activity which was maximal at 24 h of growth. Glucose concentration and the type of phosphorous source in the media modulated the enzyme activity. Fermentation of cereal and/or legume flours with the strain resulted in phytate reduction with the highest in sorghum (73%) and the lowest in horse gram (34%). Further, the strain showed tolerance to acid, bile, and simulated gastrointestinal fluid. Significant phytase activity in the presence of simulated gastrointestinal fluids along with the ability to produce phytases post-exposure to simulated gastrointestinal fluids is of interest. To the best of our knowledge, this is the first report on the effect of simulated gastrointestinal fluid on cell-associated phytases of lactobacilli. The results of the investigation indicate that L. plantarum MTCC 1325 could be used as a starter in cereal-legume fermentation and as potential probiotics to achieve phytate hydrolysis in food matrices and also in gastrointestinal tract. [ABSTRACT FROM AUTHOR]

The aim of this study was to evaluate the influence of edible coatings based on linseed mucilage, alginate, and fructooligosaccharide containing Lactobacillus casei LC-01 on the shelf-life of fresh-cut yacon cubes. The cell viability and survival under in vitro gastrointestinal conditions analysis were performed to evaluate the stability of the microorganism. To evaluate the influence of edible coatings on fresh-cut yacon, physicochemical parameters pH, acidity, soluble solids, color, and weight loss were analyzed. Edible coatings were efficient probiotic cells carrier, preserving the number of viable cells at about 8 log CFU g−1. Under simulated gastrointestinal conditions, the reduction in the number of viable cells of the microorganism was on average 2.96 log CFU g−1, indicating the yacon as viable matrix to carrier probiotic bacteria. The edible coatings helped to preserve the physicochemical parameters of the vegetable, reducing the weight loss and darkening, important factors for the commercialization of the product. [ABSTRACT FROM AUTHOR]

The protective effects of encapsulation on the survival of Lactobacillus reuteri and the retention of the bacterium's probiotic properties under simulated gastrointestinal conditions were investigated. Viable counts and the remaining probiotic properties of calcium (Ca)-alginate encapsulated (A group), chitosan-Ca-alginate encapsulated (CA group), and unencapsulated, free L. reuteri (F group) were determined. Encapsulation improved the survival of L. reuteri subjected to simulated gastrointestinal conditions, with the greatest protective effect achieved in the CA group. The degree of cell membrane injury increased with increasing bile salt concentrations at constant pH, but the extent of injury was less in the encapsulated than in the free cells. Adherence rates were, in descending order: CA (0.524%) > A (0.360%) > F (0.275%). Lactobacillus reuteri cells retained their antagonistic activity toward Listeria monocytogenes even after incubation of the Iactobacilli under simulated gastrointestinal conditions. Displacement of the pathogen by cells released from either of the encapsulation matrices was higher than that by free cells. The safety of L. reuteri was demonstrated in an in vitro invasion assay. [ABSTRACT FROM AUTHOR]

The protective effects of encapsulation on the survival of Lactobacillus reuteri and the retention of the bacterium's probiotic properties under simulated gastrointestinal conditions were investigated. Viable counts and the remaining probiotic properties of calcium (Ca)-alginate encapsulated (A group), chitosan-Ca-alginate encapsulated (CA group), and unencapsulated, free L. reuteri (F group) were determined. Encapsulation improved the survival of L. reuteri subjected to simulated gastrointestinal conditions, with the greatest protective effect achieved in the CA group. The degree of cell membrane injury increased with increasing bile salt concentrations at constant pH, but the extent of injury was less in the encapsulated than in the free cells. Adherence rates were, in descending order: CA (0.524%)>A (0.360%)>F (0.275%). Lactobacillus reuteri cells retained their antagonistic activity toward Listeria monocytogenes even after incubation of the lactobacilli under simulated gastrointestinal conditions. Displacement of the pathogen by cells released from either of the encapsulation matrices was higher than that by free cells. The safety of L. reuteri was demonstrated in an in vitro invasion assay., (Copyright© by the Spanish Society for Microbiology and Institute for Catalan Studies.)