1. Study of Butyrate Signal Transduction Pathways in Rat Hepatic Stem-Like Cells
- Author
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Ken’ichi Saito, Yusuke Mukai, Takashi Izumi, Toshihiko Saheki, Emi Tajima, Toshihiro Sugiyama, Kentaro Katakura, Atsuyoshi Nishina, Itaru Kojima, and Mikiko Kishi
- Subjects
chemistry.chemical_compound ,chemistry ,Western blot ,medicine.diagnostic_test ,Kinase ,medicine ,Phosphorylation ,Sodium butyrate ,Butyrate ,Signal transduction ,Receptor ,Molecular biology ,G protein-coupled receptor - Abstract
Short-chain fatty acids (SCFAs), acetate, propionate and butyrate, are the major anions in the lumen of the large intestine. One of the G protein coupled receptors, GPR43, was recently described as a receptor for SCFAs. To understand pharmacological role of butyrate through GPR43, we analyzed cell shape, GPR43 mRNA expression and mitogen-activated protein (MAP) kinase activation in rat hepatic stem-like (HSL) cells. RT-PCR demonstrated that GPR43 mRNA was expressed in HSL cells and the expression level was not affected by butyrate treatment. Western blot analysis revealed that ERK phosphorylation was enhanced 10 min after sodium butyrate treatment then decreased to the initial level after 24 h. In contrast, Ark phosphorylation was not detected. The results obtained were contrary to our intention, because much cell death was observed after sodium butyrate treatment.
- Published
- 2010