1. Purification and Characterisation of Sarcosine Dehydrogenase
- Author
-
Tony Atkinson, R. J. Hinton, C. P. Price, and M. D. Scawen
- Subjects
chemistry.chemical_classification ,Chromatography ,Sarcosine ,biology ,Chemistry ,Chromatofocusing ,Elution ,Fast protein liquid chromatography ,Pseudomonas fluorescens ,biology.organism_classification ,chemistry.chemical_compound ,Enzyme ,Sarcosine dehydrogenase ,Homogeneous - Abstract
Sarcosine dehydrogenase was purified to homogeneity from Pseudomonas fluorescens induced in TSB medium by sarcosine. The enzyme in a cell-free extract was initially bound to the hydrophobic matrix phenyl-Sepharose in low ionic strength buffer and eluted with Triton X-100. FPLC anion-exchange and gel-filtration was performed using Mono-Q and Superose-12 matrices respectively. Final chromatofocusing with Mono-P gave homogeneous enzyme, as shown by a single protein band silver-stained on a PAGE gel.
- Published
- 1990