1. Using FM Dyes to Monitor Clathrin-Mediated Endocytosis in Primary Neuronal Culture
- Author
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Sarah L. Gordon, Karen J. Smillie, and Michael A. Cousin
- Subjects
0301 basic medicine ,Flexibility (anatomy) ,Primary (chemistry) ,Chemistry ,Receptor-mediated endocytosis ,Endocytosis ,Fluorescence ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Membrane ,medicine.anatomical_structure ,Live cell imaging ,Biophysics ,Fluorescence microscope ,medicine ,030217 neurology & neurosurgery - Abstract
This protocol utilizes lipophilic FM dyes to monitor membrane recycling in real time. FM dyes are virtually nonfluorescent in solution but when membrane bound are intensely fluorescent, combined with the flexibility of different emission wavelengths make these dyes an excellent choice for investigating clathrin-mediated endocytosis, among other membrane trafficking and recycling pathways.
- Published
- 2018
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