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251,126 results

1. Field validation of the performance of paper-based tests for the detection of the Zika and chikungunya viruses in serum samples

Author

Massachusetts Institute of Technology. Department of Biological Engineering, Karlikow, Margot, da Silva, Severino Jefferson Ribeiro, Guo, Yuxiu, Cicek, Seray, Krokovsky, Larissa, Homme, Paige, Xiong, Yilin, Xu, Talia, Calderón-Peláez, Maria-Angelica, Camacho-Ortega, Sigrid, Ma, Duo, de Magalhães, Jurandy Júnior Ferraz, Souza, Bárbara Nayane Rosário Fernandes, de Albuquerque Cabral, Diego Guerra, Jaenes, Katariina, Sutyrina, Polina, Ferrante, Tom, Benitez, Andrea Denisse, Nipaz, Victoria, Ponce, Patricio, Rackus, Darius G, Collins, James J, Paiva, Marcelo, Castellanos, Jaime E, Cevallos, Varsovia, Green, Alexander A, Ayres, Constância, Pena, Lindomar, Pardee, Keith, Massachusetts Institute of Technology. Department of Biological Engineering, Karlikow, Margot, da Silva, Severino Jefferson Ribeiro, Guo, Yuxiu, Cicek, Seray, Krokovsky, Larissa, Homme, Paige, Xiong, Yilin, Xu, Talia, Calderón-Peláez, Maria-Angelica, Camacho-Ortega, Sigrid, Ma, Duo, de Magalhães, Jurandy Júnior Ferraz, Souza, Bárbara Nayane Rosário Fernandes, de Albuquerque Cabral, Diego Guerra, Jaenes, Katariina, Sutyrina, Polina, Ferrante, Tom, Benitez, Andrea Denisse, Nipaz, Victoria, Ponce, Patricio, Rackus, Darius G, Collins, James J, Paiva, Marcelo, Castellanos, Jaime E, Cevallos, Varsovia, Green, Alexander A, Ayres, Constância, Pena, Lindomar, and Pardee, Keith

Abstract

AbstractIn low-resource settings, resilience to infectious disease outbreaks can be hindered by limited access to diagnostic tests. Here we report the results of double-blinded studies of the performance of paper-based diagnostic tests for the Zika and chikungunya viruses in a field setting in Latin America. The tests involved a cell-free expression system relying on isothermal amplification and toehold-switch reactions, a purpose-built portable reader and onboard software for computer vision-enabled image analysis. In patients suspected of infection, the accuracies and sensitivities of the tests for the Zika and chikungunya viruses were, respectively, 98.5% (95% confidence interval, 96.2–99.6%, 268 serum samples) and 98.5% (95% confidence interval, 91.7–100%, 65 serum samples) and approximately 2 aM and 5 fM (both concentrations are within clinically relevant ranges). The analytical specificities and sensitivities of the tests for cultured samples of the viruses were equivalent to those of the real-time quantitative PCR. Cell-free synthetic biology tools and companion hardware can provide de-centralized, high-capacity and low-cost diagnostics for use in low-resource settings.

Published
2023

11. Single-step batch fabrication of microfluidic paper-based analytical devices with a 3D printer and their applications in nanoenzyme-enhanced visual detection of dopamine

Author

Yan, Yongkang, primary, Huang, Xueer, additional, Yuan, Lili, additional, Tang, Yiyue, additional, Zhu, Wenli, additional, Du, Hancong, additional, Nie, Jinfang, additional, Zhang, Lang, additional, Liao, Shan, additional, Tang, Xuehui, additional, and Zhang, Yun, additional

Published
2024
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12. Multiarmed DNA jumper and metal-organic frameworks–functionalized paper-based bioplatform for small extracellular vesicle–derived miRNAs assay

Author

Qiu, Xiaopei, primary, Yang, Huisi, additional, Shen, Man, additional, Xu, Hanqing, additional, Wang, Yingran, additional, Liu, Shuai, additional, Liu, Qian, additional, Sun, Minghui, additional, Ding, Zishan, additional, Zhang, Ligai, additional, Wang, Jun, additional, Liang, Taotao, additional, Luo, Dan, additional, Gao, Mingxuan, additional, Chen, Ming, additional, and Bao, Jing, additional

Published
2024
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