Search

Your search keyword '"Ai, Shan"' showing total 8 results
Start Over Author "Ai, Shan" Publisher springer science and business media llc
8 results on '"Ai, Shan"'

4. Interferon-α enhances sensitivity of human osteosarcoma U2OS cells to doxorubicin by p53-dependent apoptosis

Author

Gong Kan Feng, Rong Deng, Zi Bo Yang, Pu Yi Sheng, Wei Ming Liao, Xiang Wei Yuan, Xiu Fang Huang, Ai Shan He, and Xiao Feng Zhu

Subjects
Transcriptional Activation, Cell Survival, Apoptosis, In Vitro Techniques, Flow cytometry, Interferon, Cell Line, Tumor, medicine, Humans, Pharmacology (medical), Doxorubicin, Viability assay, Cytotoxicity, Pharmacology, Osteosarcoma, Antibiotics, Antineoplastic, medicine.diagnostic_test, business.industry, Interferon-alpha, Drug Synergism, General Medicine, Transfection, Genes, p53, Molecular biology, Mutation, DNA fragmentation, business, medicine.drug
Abstract

Aim: To determine whether interferon-α (IFNα) can enhance doxorubicin sensitivity in osteosarcoma cells and its molecular mechanism. Methods: Cell viability was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Apoptosis was studied using Flow cytometry analysis, Hoechst33258 staining, DNA fragmentation assay, as well as the activation of caspase-3 and poly (ADP-ribose) polymerase. Protein expression was detected by Western blotting. The dependence of p53 was determined using p53-siRNA transfection. Results: IFNα increased doxorubicin-induced cytotoxicity to a much greater degree through apoptosis in human osteosarcoma p53-wild U2OS cells, but not p53-mutant MG63 cells. IFNα markedly upregulated p53, Bax, Mdm2, and p21, downregulated Bcl-2, and activated caspase-3 and PARP cleavage in response to doxorubicin in U2OS cells. Moreover, the siRNA-mediated silencing of p53 significantly reduced the IFNα/doxorubicin combination-induced cytotoxic-ity and PARP cleavage. Conclusion: IFNα enhances the sensitivity of human osteosarcoma U2OS cells to doxorubicin by p53-dependent apoptosis. The proper combination with IFNα and conventional chemotherapeutic agents may be a rational strategy for improving the treatment of osteosarcoma with functional p53.

Published
2007

7. Proton irradiation effects on HVPE GaN

Author

Lü, Ling, primary, Hao, Yue, additional, Zheng, XueFeng, additional, Zhang, JinCheng, additional, Xu, ShengRui, additional, Lin, ZhiYu, additional, Ai, Shan, additional, and Meng, FanNa, additional

Published
2012
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results