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This pilot study sought to evaluate the feasibility of utilizing vaginal self-swabs and urine samples for HPV-based cervical cancer screening in 700 women who had undergone conventional Pap smear screening via the national cervical cancer program in Korea. The cobas 4800 HPV test was utilized to detect HPV in the self-samples. Pap smear results revealed three cases of atypical squamous cells of undetermined significance, 649 cases of negative for an intraepithelial lesion or malignancy, and 48 non-specific inflammatory findings. High-risk HPV was detected in 6.7% of urine samples and 9.6% of vaginal self-swab samples. The overall agreement for HPV 16/18 between urine and vaginal self-swab samples was 99.1% (95%CI 98.1% to 99.6%). Colposcopic biopsy revealed one cervical intraepithelial neoplasia (CIN) 3 lesion, 12 CIN1 lesions, and 23 normal or chronic cervicitis lesions. In conclusion, urine and vaginal self-swab sampling was feasible and deemed a potential alternative for HPV detection in women who hesitate to participate in cervical cancer screening programs. Meanwhile, due to overall lower rates of abnormal cytology and sexual risk behaviors in Korea, a larger sample size than expected is needed to assess the sensitivity of CIN2+ detection via self-samples.

Background Waste animal fat is a promising feedstock to replace vegetable oil that widely used in commercial biodiesel process, however the high content of free fatty acid in waste fat makes it unfeasible to be processed with commercial base-catalytic process. Enzymatic process is preferable to convert waste fat into biodiesel since enzyme can catalyze both esterification of free fatty acid and transesterification of triglyceride. However, enzymatic reaction still has some drawbacks such as lower reaction rates than base-catalyzed transesterification and the limitation of reactant concentration due to the enzyme inhibition of methanol. Supercritical CO2 is a promising reaction media for enzyme-catalyzed transesterification to overcome those drawbacks. Result The transesterification of waste animal fat was carried out in supercritical CO2 with varied concentration of feedstock and methanol in CO2. The CO2 to feedstock mass ratio of 10:1 showed the highest yield compared to other ratios, and the highest FAME yield obtained from waste animal fat was 78%. The methanol concentration effect was also observed with variation 12%, 14%, and 16% of methanol to feedstock ratio. The best yield was 87% obtained at the CO2 to feedstock ratio of 10: 1 and at the methanol to feedstock ratio of 14% after 6 h of reaction. Conclusion Enzymatic transesterification to produce biodiesel from waste animal fat in supercritical fluid media is a potential method for commercialization since it could enhance enzyme activity due to supercritical fluid properties to remove mass transfer limitation. The high yield of FAME when using high mass ratio of CO2 to oil showed that supercritical CO2 could increase the reaction and mass transfer rate while reducing methanol toxicity to enzyme activity. The increase of methanol concentration also increased the FAME yield because it might shift the reaction equilibrium to FAME production. This finding describes that the application of supercritical CO2 in the enzymatic reaction enables the application of simple process such as a packed-bed reactor.

The driving stability of a passenger car at high-speed and under crosswind conditions is affected by aerodynamic characteristics as well as their dynamic characteristics, suspension, and weight distribution. In this study, the total measuring system was thought up to understand the transient vehicle dynamics and aerodynamics with driver’s control inputs all together. The test results were taken from a full-scale wind tunnel test, a crosswind generator test and an on-road test. We investigated major aerodynamic parameters that affect the driving stability of passenger cars under crosswind effects such as overtaking, passing each other, natural crosswind, etc. The reaction rate of high-speed stability will be improved when we minimize the total lift, side force and especially the yawing moment.

Next-generation sequencing technologies (NGS) require mapping tools that are fundamental for their application. These are evaluated by the level of accuracy to be matched and read at the original location. Evaluation increases the need for a simulator to generate reads with their locations and errors, as with indel. In this paper, we propose a simulator, PSIM, that generating a set of artificial RNA segments(reads) with the expression level and errors based on a pattern-based SAM file. PSIM adopts the contour line transpose and interval section shuffle methods to generate a similar expression level. In addition, we show the similarity between a profile contour of synthesized data and a reference sequence.

In this paper, we propose an efficient iridescent rendering method. The iridescent colors on a surface is caused by the different reflection paths of rays with different wavelengths. In microfacet-based rendering model, the dominant reflection path of an incident light ray is statistically described by the microfacet-distribution function (MDF) of the surface. Therefore, the iridescent colors on the surface can be produced by applying different MDFs to different light wave channels. However, it is unnatural for a surface to have significantly different reflection properties in accordance with the light waves. Taking those into account, the proposed method employs identical and anisotropic microfacet distribution function(MDF) for each light wave channel, and rotates the identical anisotropic MDF of each channel with its own angle to produce iridescent reflection. The method also employs multi-peak MDF for the simulation of diffraction effects, and can be successfully applied to iridecent surface such as CD-ROM and mother-of-pearl (nacre) furniture. The experimental results demonstrate that the method enables interactive applications such as games or virtual reality softwares to plausibly express various cases of iridescent surfaces.

The silkworm extract powder contain 1-deoxynojirimycin (DNJ), a potent α-glycosidase inhibitor, has therapeutic potency against diabetes mellitus. Therefore, natural products containing DNJ from mulberry leaves and silkworm are consumed as health functional food. The present study was performed to evaluate the safety of the silkworm extract powder, a health food which containing the DNJ. The repeated toxicity studies and gentic toxicity studies of the silkworm extract powder were performed to obtain the data for new functional food approval in MFDS. The safety was evaluated by a single-dose oral toxicity study and a 90 day repeated-dose oral toxicity study in Sprague-Dawley rats. The silkworm extract powder was also evaluated for its mutagenic potential in a battery of genetic toxicity test: in vitro bacterial reverse mutation assay, in vitro chromosomal aberration test, and in vivo mouse bone marrow micronucleus assay. The results of the genetic toxicology assays were negative in all of the assays. The approximate lethal dose in single oral dose toxicity study was considered to be higher than 5000 mg/kg in rats. In the 90 day study, the dose levels were wet at 0, 500, 1000, 2000 mg/kg/day, and 10 animals/sex/dose were treated with oral gavage. The parameters that were monitored were clinical signs, body weights, food and water consumptions, ophthalmic examination, urinalysis, hematology, serum biochemistry, necropsy findings, organ weights, and histopathological examination. No adverse effects were observed after the 90 day administration of the silkworm extract powder. The No-Observed-Adverse-Effect-Level (NOAEL) of silkworm extract powder in the 90 day study was 2000 mg/kg/day in both sexes, and no target organ was identified.

A semi-pilot continuous process (SPCP) for enzymatic biodiesel synthesis utilizing near-critical carbon dioxide (NcCO₂) as the reaction medium was developed with the aim of reducing the reaction time and alleviating the catalyst inhibition by methanol. Biodiesel synthesis was evaluated in both lab-scale and semi-pilot scale reactors (batch and continuous reactors). In a SPCP, the highest conversion (∼99.9 %) in four and a half hours was observed when three-step substrate (methanol) addition (molar ratio [oil/methanol] = 1:1.3) was used and the reaction mixture containing enzyme (Lipozyme TL IM, 20 wt.% of oil) was continuously mixed (agitation speed = 300 rpm) at 30 °C and 100 bar in a CO₂ environment. The biodiesel produced from canola oil conformed to the fuel standard (EU) even without additional downstream processing, other than glycerol separation and drying.

This study proposes an aerodynamically optimized outer shape of a sedan by using an Artificial Neural Network (ANN), which focused on modifying the rear body shapes of the sedan. To determine the optimization variables, the unsteady flow field around the sedan driving at very fast speeds was analyzed by CFD simulation, and fluctuations of the drag coefficient (C D ) and pressure around the car were calculated. After consideration of the baseline result of CFD, 6 local parts from the end of the sedan were chosen as the design variables for optimization. Moreover, an ANN approximation model was established with 64 experimental points generated by the D-optimal methodology. As a result, an aerodynamically optimized shape for the rear end of the sedan in which the aerodynamic performance is improved by about 5.64% when compared to the baseline vehicle is proposed. Finally, it is expected that within the accepted range of shape modifications for a rear body, the aerodynamic performance of a sedan can be enhanced so that the fuel efficiency of the sedan can be improved. The YF SONATA, a sedan manufactured by Hyundai Motors Corporate, played a major role in this research as the baseline vehicle.

This research aims to develop an actively translating rear diffuser device to reduce the aerodynamic drag experienced by passenger cars. One of the features of the device is that it is ordinarily hidden under the rear bumper but slips out backward only under high-speed driving conditions. In this study, a movable arc-shaped semi-diffuser device, round in form, is designed to maintain the streamlined automobile’s rear underbody configuration. The device is installed in the rear bumper section of a passenger car. Seven types of rear diffuser devices whose positions and protrusive lengths and widths are different (with the basic shape being identical) were installed, and Computational Fluid Dynamics (CFD) analyses were performed under moving ground and rotating wheel conditions. The main purpose of this study is to explain the aerodynamic drag reduction mechanism of a passenger car cruising at high speed via an actively translating rear diffuser device. The base pressure of the passenger car is increased by deploying the rear diffuser device, which then prevents the low-pressure air coming through the underbody from directly soaring up to the rear surface of the trunk. At the same time, the device generates a diffusing process that lowers the velocity but raises the pressure of the underbody flow, bringing about aerodynamic drag reduction. Finally, the automobile’s aerodynamic drag is reduced by an average of more than 4%, which helps to improve the constant speed fuel efficiency by approximately 2% at a range of driving speeds exceeding 70 km/h.

Efficient binding of enzymes onto the electrode surface has been prerequisite for the construction of sensitive biosensors and biochips. Here, a simple and robust construction of electrochemical glucose biosensor based on pyrroloquinoline quinone-glucose dehydrogenase was demonstrated. The glucose biosensor was fabricated by binding the enzyme onto the anionic self-assembled monolayers on gold electrode via electrostatic interactions. The resulting glucose biosensor gave rise to twofold higher detection sensitivity than that by covalent conjugation under the same condition. Surface plasmon resonance and atomic force microscopy analyses revealed that electrostatic binding of the enzyme leads to much higher surface density of the enzyme. This approach will find wide applications to the development of robust enzyme-based biosensors and biochips.

The enzymatic synthesis of biodiesel by a high-pressure semi-continuous process in near-critical carbon dioxide (NcCO(2)) was studied. Biodiesel synthesis was evaluated in both batch and semi-continuous systems to develop an effective process. Batch processing demonstrated the advantageous properties of NcCO(2) as an alternative reaction medium. Three immobilized lipases (Novozym 435, Lipozyme RM IM, and Lipozyme TL IM from Novozymes) were tested, with Lipozyme TL IM the most effective, showing the highest conversion. Biodiesel conversion from several edible and non-edible oil feedstocks reached92%. Higher conversion (99.0%) was obtained in a shorter time by employing repeated batch processes with optimized conditions: 44.3 g (500 mM) canola oil, a substrate molar ratio (methanol:oil) of 3:1, an enzyme loading of 20 wt% (of the oil used), at 30 °C, 100 bar, and 300 rpm agitation. The enzyme maintained 80.2% of its initial stability after being reused eight times. These results suggest that this method produces biodiesel energy-efficiently and environment-friendly.

Rhizopus oryzae RUR709 mutant was isolated based on halo size from selection medium via mutagenesis with UV and γ-rays, and the production of fumaric acid in the submerged fermentation was assessed. The maximum concentration of fumaric acid was obtained using 0.5% corn steep liquor (CSL) as the nitrogen source. Organic nitrogen sources were shown to be more effective in fumaric acid production than inorganic nitrogen sources. Using optimum medium obtained by response surface methodology (RSM), the maximum concentration of fumaric acid achieved in flask culture was 26.2 g/L, which is fairly close to the 27.4 g/L predicted by the model. The highest concentration of fumaric acid in the stirred-tank reactor generated by the R. oryzae RUR709 mutant was 32.1 g/L and yield (0.45 g/g) and productivity (0.32 g/L/h) were highest at 4 days.

On-line monitoring method of the denitrification process was developed by NADH fluorescence measurements using the facultative microorganism, Ochrobactrum anthropi SY509. The NADH fluorescence signals showed a rapid drop and a rise at the initiation and termination points of the denitrification, respectively. This NADH fluorescence method could be applied successfully to the monitoring of the denitrification process in sequential batch and continuous operations while these rapid changes of fluorescence were not observed in a batch operation due to accumulation of some metabolites secreted from the microorganism.

This paper presents an efficient method for creating the animation of flexible objects. The mass-spring model was used to represent flexible objects. The easiest approach to creating animation with the mass-spring model is the explicit Euler method, but the method has a serious weakness in that it suffers from an instability problem. The implicit integration method is a possible solution, but a critical flaw of the implicit method is that it involves a large linear system. This paper presents an approximate implicit method for the mass-spring model. The proposed technique updates with stability the state of n mass points in O(n) time when the number of total springs is O(n). In order to increase the efficiency of simulation or reduce the numerical errors of the proposed approximate implicit method, the number of mass points must be as small as possible. However, coarse discretization with a small number of mass points generates an unrealistic appearance for a cloth model. By introducing a wrinkled cubic spline curve, we propose a new technique that generates realistic details of the cloth model, even though a small number of mass points are used for simulation.

Sequence distributions of the 1-butene comonomers in ethylene-1-butene copolymers were determined by analyzing the 13C NMR spectra using an optimization calculation technique. The proposed method takes the possible errors in NMR measurements into account. Applying the method to the spectra of some linear low density polyethylenes with 1-butene as the comonomer gave physically reasonable values of triad comonomer distribution. The method was thought to be useful by giving the possible maximum and minimum triad concentrations reflecting the chain structures. We also tried to use the method for determination of larger sequence structures over triad distribution.

Background Three phenotypic identification systems (MicroScan, VITEK 2, and Crystal GP) were evaluated for their accuracy to identify coagulase-negative staphylococci (CNS). A total of 120 clinical isolates confirmed to be CNS via 16S rRNA sequencing and analysis with the MicroSeq 500 v2.0 database were assessed. Results The MicroScan, VITEK 2, and Crystal GP systems correctly identified 82.5%, 87.5%, and 67.5% of the isolates, respectively. Misidentification was the main problem in MicroScan (10.8%) and Crystal GP (23.3%) systems, whereas the main problem of VITEK 2 was low-level discrimination (7.5%). Conclusion None of the 3 phenotypic systems tested could accurately and reliably identify CNS at the species level. Further verifications such as biochemical testing or 16S rRNA sequencing together with analysis using a comparable database might be helpful in this regard.