1. Detection of gut microbiota and pathogen produced N-acyl homoserine in host circulation and tissues
- Author
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Jingchuan Xue, Kun Lu, Chih-Wei Liu, Hongyu Ru, Pengcheng Tu, Liang Chi, and Yunjia Lai
- Subjects
Cell signaling ,Homoserine ,Gut flora ,medicine.disease_cause ,digestive system ,Applied Microbiology and Biotechnology ,Microbiology ,Article ,Microbial ecology ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,4-Butyrolactone ,Tandem Mass Spectrometry ,medicine ,Citrobacter rodentium ,Animals ,Metabolomics ,Pathogen ,Chromatography, High Pressure Liquid ,030304 developmental biology ,0303 health sciences ,Molecular Structure ,biology ,030306 microbiology ,QR100-130 ,Biofilm ,food and beverages ,Pathogenic bacteria ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,Gastrointestinal Microbiome ,Crosstalk (biology) ,chemistry ,Organ Specificity ,Biofilms ,Host-Pathogen Interactions ,Microbiome ,Pathogens ,Biomarkers ,Biotechnology - Abstract
Recent studies suggest that quorum-sensing molecules may play a role in gut microbiota-host crosstalk. However, whether microbiota produces quorum-sensing molecules and whether those molecules can trans-kingdom transport to the host are still unknown. Here, we develop a UPLC-MS/MS-based assay to screen the 27 N-acyl homoserine lactones (AHLs) in the gut microbiota and host. Various AHL molecules are exclusively detected in the cecal contents, sera and livers from conventionally-raised mice but cannot be detected in germ-free mice. Pathogen-produced C4-HSL is detected in the cecal contents and sera of Citrobacter rodentium (C. rodentium)-infected mice, but not found in uninfected controls. Moreover, C. rodentium infection significantly increases the level of multiple AHL molecules in sera. Our findings demonstrate that both commensal and pathogenic bacteria, can produce AHLs that can be detected in host bodies, suggesting that quorum-sensing molecules could be a group of signaling molecules in trans-kingdom microbiota-host crosstalk.
- Published
- 2021