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Peatlands play a crucial role in mediating the emissions of methane through active biogeochemical cycling of accumulated carbon driven by methane-metabolizing microorganisms; meanwhile, they serve as vital archives of atmospheric heavy metal deposition. Despite many edaphic factors confirmed as determinants to modulate the structure of methanotrophic and methanogenic communities, recognition of interactions among them is limited. In this study, peat soils were collected from Dajiuhu peatland to assess the presence of heavy metals, and methanotrophs and methanogens were investigated via high-throughput sequencing for functional genes mcrA and pmoA. Further analyses of the correlations between methane-related functional groups were conducted. The results demonstrated that both methane-metabolizing microorganisms and heavy metals have prominent vertical heterogeneity upward and downward along the depth of 20 cm. Pb, Cd, and Hg strongly correlated with methanotrophs and methanogens across all seasons and depths, serving as forceful factors in structural variations of methanogenic and methanotrophic communities. Particularly, Pb, Cd, and Hg were identified as excessive elements in Dajiuhu peatland. Furthermore, seasonal variations of networks among methane-related functional groups and environmental factors significantly affected the changes of methane fluxes across different seasons. Concretely, the complicated interactions were detrimental to methane emissions in the Dajiuhu peatland, leading to the minimum methane emissions in winter. Our study identified the key heavy metals affecting the composition of methane-metabolizing microorganisms and linkages between seasonal variations of methane emissions and interaction among heavy metals and methane-metabolizing microorganisms, which provided much new reference and theoretical basis for integrated management of natural peatlands.

Cylindrospermopsis raciborskii and its highly similar relatives Raphidiopsis species have been recognized as globally invasive and expansive filamentous cyanobacteria causing water blooms. Reports on C. raciborskii/Raphidiopsis species and their harmful metabolites such as hepatotoxic cylindrospermopsins (CYNs) in Chinese waters have been increasing, but mostly restricted to the southern regions of China. To further explore the existence and distribution of C. raciborskii in China, six water samples from Beijing city were morphologically and molecularly examined. Five samples of the six were shown to have Cylindrospermopsis filaments with straight and spiral morphotypes. PCR detection targeting on Cylindrospermopsis/Raphidiopsis specific 16S rRNA gene region also showed the positive amplification, and such amplifications were confirmed by sequencing and phylogenetic analysis. As well, three of the five Cylindrospermopsis containing samples were shown to have cyrJ — a gene of CYN synthesis gene cluster. The results represented the presence of toxic Cylindrospermopsis at the most northern line in China so far, indicating rapid expansion of this harmful invasive cyanobacterium. It is strongly suggested that the monitoring on C. raciborskii/Raphidiopsis species and their production of cylindrospermopsin should be emphasized in Beijing and even more northern parts of China.

Magnetic reconnection provides a physical mechanism for fast energy conversion from magnetic energy to plasma kinetic energy. It is closely associated with many explosive phenomena in space plasma, usually collisionless in character. For this reason, researchers have become more interested in collisionless magnetic reconnection. In this paper, the various roles of electron dynamics in collisionless magnetic reconnection are reviewed. First, at the ion inertial length scale, ions and electrons are decoupled. The resulting Hall effect determines the reconnection electric field. Moreover, electron motions determine the current system inside the reconnection plane and the electron density cavity along the separatrices. The current system in this plane produces an out-of-plane magnetic field. Second, at the electron inertial length scale, the anisotropy of electron pressure determines the magnitude of the reconnection electric field in this region. The production of energetic electrons, which is an important characteristic during magnetic reconnection, is accelerated by the reconnection electric field. In addition, the different topologies, temporal evolution and spatial distribution of the magnetic field affect the accelerating process of electrons and determine the final energy of the accelerated electrons. Third, we discuss results from simulations and spacecraft observations on the secondary magnetic islands produced due to secondary instabilities around the X point, and the associated energetic electrons. Furthermore, progress in laboratory plasma studies is also discussed in regard to electron dynamics during magnetic reconnection. Finally, some unresolved problems are presented.