1. Serial two-photon tomography for automated ex vivo mouse brain imaging
- Author
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Yongsoo Kim, H. Sebastian Seung, Timothy Ragan, Jason Sutin, Karsten Bahlmann, Ignacio Arganda-Carreras, Kannan Umadevi Venkataraju, Lolahon R. Kadiri, Pavel Osten, and Julian Taranda
- Subjects
0303 health sciences ,Fluorescence-lifetime imaging microscopy ,Cell Biology ,Human brain ,Anatomy ,Biology ,Biochemistry ,03 medical and health sciences ,0302 clinical medicine ,medicine.anatomical_structure ,Neuroimaging ,Two-photon excitation microscopy ,Microscopy ,medicine ,Tomography ,Molecular Biology ,030217 neurology & neurosurgery ,Ex vivo ,030304 developmental biology ,Biotechnology ,Neuroanatomy ,Biomedical engineering - Abstract
Here we describe an automated method, named serial two-photon (STP) tomography, that achieves high-throughput fluorescence imaging of mouse brains by integrating two-photon microscopy and tissue sectioning. STP tomography generates high-resolution datasets that are free of distortions and can be readily warped in three dimensions, for example, for comparing multiple anatomical tracings. This method opens the door to routine systematic studies of neuroanatomy in mouse models of human brain disorders.
- Published
- 2012
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