1. Canadian macromolecular crystallography facility: a suite of fully automated beamlines
- Author
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Shaunivan Labiuk, Kathryn Janzen, Pawel Grochulski, Michel Fodje, James Gorin, and Russ Berg
- Subjects
Canada ,Protein Folding ,Macromolecular Substances ,Computer science ,Peptides, Cyclic ,Biochemistry ,Structural Biology ,Escherichia coli ,Genetics ,Simulation ,Electronic Data Processing ,Data processing ,Crystallography ,Data collection ,business.industry ,Suite ,Software development ,Robotics ,General Medicine ,Undulator ,Automation ,Visualization ,Phosphotransferases (Alcohol Group Acceptor) ,Beamline ,Magnets ,Systems engineering ,business ,Software ,Synchrotrons ,Molecular Chaperones - Abstract
The Canadian light source is a 2.9 GeV national synchrotron radiation facility located on the University of Saskatchewan campus in Saskatoon. The small-gap in-vacuum undulator illuminated beamline, 08ID-1, together with the bending magnet beamline, 08B1-1, constitute the Canadian Macromolecular Crystallography Facility (CMCF). The CMCF provides service to more than 50 Principal Investigators in Canada and the United States. Up to 25% of the beam time is devoted to commercial users and the general user program is guaranteed up to 55% of the useful beam time through a peer-review process. CMCF staff provides "Mail-In" crystallography service to users with the highest scored proposals. Both beamlines are equipped with very robust end-stations including on-axis visualization systems, Rayonix 300 CCD series detectors and Stanford-type robotic sample auto-mounters. MxDC, an in-house developed beamline control system, is integrated with a data processing module, AutoProcess, allowing full automation of data collection and data processing with minimal human intervention. Sample management and remote monitoring of experiments is enabled through interaction with a Laboratory Information Management System developed at the facility.
- Published
- 2012
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