1. Identification of marker genes and pathways specific to precancerous duodenal adenomas and early stage adenocarcinomas
- Author
-
Ken Ichi Inada, Yoshiki Sakaguchi, Shuta Tomida, Kazuhiko Koike, Kazuya Shiogama, Nobutake Yamamichi, Mitsuhiro Fujishiro, Masao Ichinose, Natsuko Kageyama-Yahara, Chihiro Takeuchi, and Yu Takahashi
- Subjects
Adenoma ,Adult ,Male ,Pilot Projects ,Adenocarcinoma ,Biology ,medicine.disease_cause ,03 medical and health sciences ,Duodenal Adenoma ,0302 clinical medicine ,Duodenal Neoplasms ,Gene expression ,medicine ,Humans ,Prospective Studies ,Intestinal Mucosa ,Wnt Signaling Pathway ,beta Catenin ,Gene knockout ,Aged ,Neoplasm Staging ,Aged, 80 and over ,Gastroenterology ,Wnt signaling pathway ,KLK6 ,Middle Aged ,digestive system diseases ,Up-Regulation ,Gene expression profiling ,030220 oncology & carcinogenesis ,Cancer research ,Female ,030211 gastroenterology & hepatology ,Duodenal adenocarcinoma ,Colorectal Neoplasms ,Transcriptome ,Carcinogenesis ,Precancerous Conditions - Abstract
The mechanism behind the pathogenesis and carcinogenesis of these neoplasms is not fully understood. The objective of this study was to identify genetic markers and pathways specific to precancerous duodenal adenomas and early stage adenocarcinomas through gene expression analysis. Gene expression profiling was performed in 4 pairs of duodenal adenoma/adenocarcinomas and corresponding matched normal tissue. Genes with consistent expression differences were identified and confirmed in 7 independent pairs. Gene set enrichment analysis (GSEA) was performed to characterize gene expression profiles of duodenal adenoma/adenocarcinomas, together with immunohistochemical staining of candidate oncogenic genes. 626 probes consistently demonstrated over a twofold expression difference between tumor–normal pairs. Reverse transcriptase polymerase chain reaction of genes with the most prominent difference in expression between tumors and normal mucosa (KLK7, KLK6, CEMIP, MMP7, KRT17, LGR5, G6PC, S100G, APOA1) validated the results of gene expression analysis. GSEA demonstrated a strong association between duodenal adenoma/adenocarcinomas with colorectal adenomas (p
- Published
- 2018