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Objectives This study was designed to investigate to test the effect of exosomes from urine-derived mesenchymal stem cells (USCs) on the survival and viability of aging retinal ganglion cells (RGCs), and explored the preliminary related mechanisms. Methods Primary USCs were cultured and identified by immunofluorescence staining. Aging RGCs models were established by D-galactose treatment and identified by β-Galactosidase staining. After treatment with USCs conditioned medium (with USCs removal), flow cytometry was performed to examine the apoptosis and cell cycle of RGCs. Cell viability of RGCs was detected by Cell-counting Kit 8 (CCK8) assay. Moreover, gene sequencing and bioinformatics analysis were applied to analyze the genetic variation after medium treatment in RGCs along with the biological functions of differentially expressed genes (DEGs). Results The number of apoptotic aging RGCs was significantly reduced in USCs medium-treated RGCs. Besides, USCs-derived exosomes exert significant promotion on the cell viability and proliferation of aging RGCs. Further, sequencing data analyzed and identified DEGs expressed in aging RGCs and aging RGCs treated with USCs conditioned medium. The sequencing outcomes demonstrated 117 upregulated genes and 186 downregulated genes in normal RGCs group vs aging RGCs group, 137 upregulated ones and 517 downregulated ones in aging RGCs group vs aging RGCs + USCs medium group. These DEGs involves in numerous positive molecular activities to promote the recovery of RGCs function. Conclusions Collectively, the therapeutic potentials of USCs-derived exosomes include suppression on cell apoptosis, enhancement on cell viability and proliferation of aging RGCs. The underlying mechanism involves multiple genetic variation and changes of transduction signaling pathways.

For the first time, novel electrocatalysts MoS2/CoSe2-x (x are the weight ratio of MoS2/CoSe2, x = 15%, 25%, 35%) have been synthesized by solvothermal method, which are facile, low-cost, and easy to realize scalable production. While the results of electrochemical testings show that composition can improve the properties, the ratio of hybrids can effect the catalytic performances. And when the x is 25% (MoS2/CoSe2-25), the hybrid shows the best catalytic performances towards hydrogen evolution reaction (HER), with a low overpotential of 113 mV at 10 mA/cm2, the smaller Tafel slope (45 mV/dec), and kinetic resistance (23 Ω), and the current density can be maintained for at least 24 h. Thus, MoS2/CoSe2-25 shows potential application in water splitting.

Poly(ferric 2-acrylamido-2-methylpropanesulfonate) (PFAMPS) was prepared via the reaction of poly(2-acrylamido-2-methylpropanesulfonic acid) and Fe(OH)3. PFAMPS was used as a heterogeneous catalyst for the Biginelli reaction of aldehyde, ethyl acetoacetate, and urea to synthesize 3,4-dihydropyrimidin-2(1H)-ones with yields of 70–87%. PFAMPS can be recycled six times without significant loss in catalytic activity. For comparison, ferric lignosulfonate (FLSA), ferric cellulose sulfonate (FCSA), ferric starch sulfonate (FSSA), and ferric 732 cation exchange resin (FACER) were used as catalysts of the Biginelli reaction. It was shown that the catalytic activities of recycled FLSA, FCSA, FSSA, and FACER significantly decreased after two reactions. Fe3+ in the polymer sulfonates acted as the catalyst for the Biginelli reaction. PFAMPS was the chelate, where the action between Fe3+ and the poly(2-acrylamido-2-methylpropanesulfonate) matrix was strong, and Fe3+ lost little from the PFAMPS. However, significant Fe3+ loss was observed in the FLSA, FCSA, FSSA and FACER polymer matrices after reuse.

Diagenetic fluid types of the Cretaceous Bashijiqike formation are restored based on the analysis of petrographic, electron microprobe composition, inclusions homogenization temperature, salinity and vapor composition and laser carbon and oxygen isotope of diagenetic mineral, and regional geological background. Diagenetic fluid evolution sequence is analyzed on this basis. The crystalline dolomite cement has a low concerntration of Sr, high concerntration of Mn and higher carbon isotope, showing that the crystalline dolomite is affected by meteoric fresh water, associated with the tectonic uplift of late Cretaceous. Similar δ13CPDB, negative transfer of δ18OPDB and the differentiation of the concerntration of Fe and Mn indicate that the diagenetic fluid of the vein dolomite cement is homologous with the diagenetic fluid of the crystalline dolomite cement, temperature and depth are the dominant factors of differential precipitation between these two carbonate cements. Anhydrite cements have high concerntration of Na, extremely low concerntration of Fe and Mn contents. Based on these data, anhydrite cements can be thought to be related to the alkaline fluid overlying gypsum-salt layer produced by dehydration. The barite vein has abnormally high concerntration of Sr, ultra-high homogenization temperature and high-density gas hydrocarbon inclusions, which is speculated to be the forward fluid by intrusion of late natural gas. Coexistence of methane inclusions with CO2 gas proves existence of acid water during the accumulation of natural gas in the late stages. Therefore, the alkaline environment and associated diagenesis between the meteoric fresh water in epidiagentic stage and carbonic acid in the late diagenesis have dominated the process of diagenesis and reservoir, the secondary porosity and fracture zone formed by gas accumulation is a favorable play for the exploration of ultra-deep reservoirs.

In this paper, the CHAMP satellite data from the last decade are used to map the lithospheric magnetic field in continental China at altitudes of 300 and 400 km using the spline function model technique. The results show that our model has higher amplitude than the spherical harmonic magnetic field model MF7. The resulting lithospheric anomaly distribution of continental China agrees with the geotectonic structure and crustal thermal structure. The magnetic anomaly distribution clearly indicates extrusion of the Tibetan Plateau towards the northeast and northwest directions. Northward movement is impeded by the relative rigid Tarim Basin featured with positive magnetic anomalies. The positive magnetic anomalies in Tarim basin extend to the northern Tibetan Plateau, suggesting that the southern part of Tarim Basin underthrusted the northern Tibetan Plateau.

This work presents a highly flexible mixed-signal CMOS image sensor suitable for smart camera applications. These systems need to fit different constraints regarding power consumption, speed and quality, and the optimal compromise may differ depending on the application. Moreover, the best implementation of a desired image processing task may be in the analog or the digital domain, or even a combined computation. Different aspects starting from the image sensor and signal acquisition up to the pre-processing in analog and digital domain are investigated in this paper to optimize not just one part of the system, but the whole system altogether. Moreover, it is shown that analog processing algorithms can improve signal quality, processing speed and latency while being able to save power, which is important for real-time systems. In order to be able to carry out spatial operations, the state-of-the-art sensor is modified to be able to read out multiple pixels at the same time. This allows analog spatial filter operations which consume significantly less power. As an example, an averaging filter is described which needs less than 5.3 % of the power–time product of a digital implementation for one computation. To enhance data throughput and flexibility, 3D chip stacking is proposed to partition the sensor in smaller units and enable massively parallel processing.

The hazardous substance from Procambarus clarkii could cause rhabdomyolysis. Mice were administered mashed muscles and gills, intestines, and glands (GIG) obtained from cooked crayfish by gavage . Three of seventy-two mice administered with GIG (with an incidence rate of 4.17 %) appeared to have rhabdomyolysis symptoms with the serum creatine kinase (CK) content increased by more than 5 times, as well as the pathological changes in livers, spleens, kidneys, and skeletal muscles. Furthermore, the cytotoxicity and hemolysis analysis of the palytoxin revealed that the methanol/water extracts from GIG of crayfish were not inhibited by ouabain effectively, which pointed out the hazardous substance causing rhabdomyolysis was not allergen or palytoxin. These research results could be valuable in further comprehensive experiments and lay a foundation for basic rhabdomyolysis research.

Mortgage banking subsidiaries of bank holding companies (BHCs) became increasingly active players in the mortgage origination market during the last decade. At the time, the Federal Reserve Board (FRB) had supervisory power over BHCs, and under the Bank Holding Company Act was authorized to monitor and examine the subsidiaries of BHCs under certain circumstances. However, this authority did not clearly extend to routine examinations of nonbank subsidiaries of BHCs. This changed between 2007 and 2009 due to FRB policy on consumer compliance supervision for nonbank subsidiaries of BHCs. Our empirical analysis tests for the impact of the new policy on the quantity and quality of loan originations by mortgage banking subsidiaries of BHCs. We use a difference-in-differences estimator with the control group being other types of lenders. We show that loan production moved from mortgage banking subsidiaries to their affiliated depository institutions after the policy change. The quality of loans originated by mortgage banking subsidiaries of BHCs increased after the policy change: The denial rates for mortgage applications increased, loan-to-income ratios decreased, and the proportion of owner occupied houses increased in mortgage banking subsidiaries more than they did in other types of lenders.

Although SIRT7 is a member of sirtuin family proteins that are described as NAD+-dependent class III histone deacetylases, the intrinsic enzymatic activity of this sirtuin protein remains to be investigated and the cellular function of SIRT7 remains to be explored. Here we report that SIRT7 is an NAD+-dependent histone desuccinylase. We show that SIRT7 is recruited to DNA double-strand breaks (DSBs) in a PARP1-dependent manner and catalyses desuccinylation of H3K122 therein, thereby promoting chromatin condensation and DSB repair. We demonstrate that depletion of SIRT7 impairs chromatin compaction during DNA-damage response and sensitizes cells to genotoxic stresses. Our study indicates SIRT7 is a histone desuccinylase, providing a molecular basis for the understanding of epigenetic regulation by this sirtuin protein. Our experiments reveal that SIRT7-catalysed H3K122 desuccinylation is critically implemented in DNA-damage response and cell survival, providing a mechanistic insight into the cellular function of SIRT7., SIRT7 is a member of sirtuin family proteins that are described as NAD+-dependent class III histone deacetylases. Here, the authors show that SIRT7 is histone desuccinylase catalysing H3K122 desuccinylation, thereby promoting chromatin condensation and repair of DNA double strand breaks.

This study is mainly aimed at evaluating the expression of PC-cell-derived growth factor (PCDGF) in breast cancer and breast adenofibroma, and to compare with other commonly used clinical pathological indices, then to investigate the diagnostic and targeted therapeutic purpose of PCDGF in breast cancer tissue. In this study, we detected the expression of PCDGF, p53 and CerbB-2 in breast cancer tissue and the expression of PCDGF in breast adenofibroma tissue by immunohistochemical method, and analyzed the relationship between them. We found that PCDGF was expressed in most breast cancer tissue, but was not in breast adenofibroma tissue, and the expression of PCDGF was related with the tumor’s pathological category and the expression of estrogen receptor (ER) and progesterone receptor (PR) and p53, but there was no statistical dependability between PCDGF and cerbB-2. From this study, we predict that PCDGF may serve as a marker in the secondary diagnosis of breast cancer, and may participate in the generation and differentiation of breast cancer cells, and become an effective target of therapy for breast cancer.

Phosphatidylinositol-4-phosphate 5-kinase-like 1 (PIP5KL1), the forth member of phosphatidylinositol-4-phosphate 5-kinases (PIPKs) type I, acts as a scaffold for localization and activation of PIPKs, which mediates numerous cellular processes. However, the role of PIP5KL1 in the development of human cancer is still lacking. We therefore examined the expression of PIP5KL1 in human normal and cancer tissues by tissue microarrays (TMAs). Reverse transcription-polymerase chain reaction (RT-PCR) and immunofluorescence imaging analysis were used to testify the mRNA and protein levels of PIP5KL1 in human gastric cancer cell line (BGC823). The cell proliferation was investigated with 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay. Both wound healing and transwell migration assay were performed to study the cell migration. The phosphorylation of v-akt murine thymoma viral oncogene homolog 1 (AKT1) was determined by western immunoblot analysis. Immunostaining of gastric cancer tissue microarrays revealed a negative correlation between PIP5KL1 overexpression and gastric cancer in situ. Transient transfection PIP5KL1 induced a significant increase expression at both transcriptional and translational levels and consequent robust inhibition of proliferation (P

The recovery of motor function in rats is inhibited following contusion spinal cord injury (cSCI). However, the mechanism of tumour necrosis factor α (TNF-α) in motor function after cSCI associated with peroxiredoxin 6 (PRDX6) remains unknown. We randomly divided rats into four groups: sham, cSCI, vector and lentivirus mediating TNF-α RNA interference (TNF-α-RNAi-LV) group. The Basso, Beattie, Bresnahan (BBB) scale was used to evaluate motor function. Real-time quantitative PCR (qRT-PCR) and western blotting were used to detect the expression of TNF-α and PRDX6, which were located in neurons using immunohistochemistry (IHC) and immunofluorescence. Subsequently, lentiviral-mediated TNF-α was used to determine the role of TNF-αand the relationship of PRDX6 and TNF-α in cSCI. After cSCI, the motor capability of hind limbs disappeared and was followed by recovery of function. IHC analysis indicated that TNF-α and PRDX6 were primarily located in spinal cord neurons. TNF-α interference significantly improved neural behaviour and increased expression of PRDX6. Our study suggests that inhibition of TNF-α can promote the recovery of motor function. The underlying mechanism of TNF-α-promoted motor function may be connected with the up-regulation of PRDX6. This provides a new strategy or target for the clinical treatment of SCI in future.

Tumor suppressor genes play a key role in cancer pathogenesis. Through massive expression profiling we identified CHAC2 as a frequently downregulated gene in gastric and colorectal cancers. Immunohistochemistry and western blot revealed that CHAC2 was downregulated in most tumor tissues, and 3-year survival rate of patients with high CHAC2 expression was significantly higher than that of patients with low CHAC2 expression (PP=0.001, respectively). The data of univariate analysis and multivariate analysis suggested that CHAC2 could serve as an independent prognostic marker. Our results showed for the first time that CHAC2 was degraded by the ubiquitin-proteasome pathway and CHAC2 expression inhibited tumor cell growth, proliferation, migration in vitro and in vivo. Mechanistic study showed that CHAC2 induced mitochondrial apoptosis and autophagy through unfolded protein response. So in gastric and colorectal cancer CHAC2 acted as a tumor suppressor and might have therapeutic implication for patients.

In order to investigate the relationship between the extent of tumor invasion and the tumor size, axillary lymph nodes metastasis, Her-2 gene overexpression, and histologic grading in breast invasive ductal carcinoma as well as the optimal extent of excision during the breast-serving surgery, the clinical data of 104 patients with breast invasive ductal carcinoma who had received modified radical mastectomy were analyzed. The correlation analysis on invasive extent, which was evaluated by serial sections at an interval of 0.5 cm from 4 different directions taking the focus as the centre, and the tumor size, axillary lymph nodes metastasis, Her-2 gene overexpression, and histologic grading was processed. There was a significant correlation between invasive extent and tumor size (r=0.766, P0.01), and lymph nodes metastases (r=0.574, P0.01), but there was no significant correlation between invasive extent and Her-2 expression (r=0.106, P0.05), and histologic grading (r=0.228, P0.05). The 100% negative rate of infiltration in patients without nipple discharge with tumor size2, 2-3 and3 cm was obtained at 1.5, 2.0 and 2.5 cm away from the tumor respectively. It is concluded that the performance of breast-serving surgery in patients with breast invasive ductal carcinoma should be evaluated by tumor size in combination with axillary lymph nodes involvement to decide the possibility of breast-serving and the secure excision extent.