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Your search keyword '"Le Gac, Séverine"' showing total 13 results
Start Over Author "Le Gac, Séverine" Publisher springer science and business media llc
13 results on '"Le Gac, Séverine"'

4. Organosilicon uptake by biological membranes

Author

Beekman, Pepijn, primary, Enciso-Martinez, Agustin, additional, Pujari, Sidharam P., additional, Terstappen, Leon W. M. M., additional, Zuilhof, Han, additional, Le Gac, Séverine, additional, and Otto, Cees, additional

Published
2021
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9. An oviduct-on-a-chip provides an enhanced in vitro environment for zygote genome reprogramming

Author

Ferraz, Marcia A M M, Rho, Hoon Suk, Hemerich, Daiane, Henning, Heiko H W, van Tol, Helena T A, Hölker, Michael, Besenfelder, Urban, Mokry, Michal, Vos, Peter L A M, Stout, Tom A E, Le Gac, Séverine, Gadella, Bart M, LS Voortplanting Inwendige Ziekten, dES/dFAH FR, Sub Celbiologisch lab., dFAH AVR, LS Klinische Reproductie, Sub Biologie van de mannelijke gameet, Sub Reproductie mannelijk, dB&C FR-RMSC FR, LS Voortplanting Inwendige Ziekten, dES/dFAH FR, Sub Celbiologisch lab., dFAH AVR, LS Klinische Reproductie, Sub Biologie van de mannelijke gameet, Sub Reproductie mannelijk, and dB&C FR-RMSC FR

Subjects
0301 basic medicine, Zygote, medicine.medical_treatment, General Physics and Astronomy, Oviducts, Cellular Reprogramming/genetics, Epigenesis, Genetic, Human fertilization, Pregnancy, Zygote/growth & development, Non-U.S. Gov't, lcsh:Science, Cells, Cultured, Cultured, Multidisciplinary, Research Support, Non-U.S. Gov't, Embryo, Genomics, Cellular Reprogramming, 3. Good health, Cell biology, Fertilization in Vitro/instrumentation, Oviduct, Oviducts/cytology, Female, Reprogramming, Science, Cells, Fertilization in Vitro, Biology, Research Support, Article, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, Genetic, Journal Article, medicine, Animals, Humans, Assisted reproductive technology, Gene Expression Profiling, Embryo culture, General Chemistry, Embryonic stem cell, Genomics/methods, Gene Ontology, 030104 developmental biology, lcsh:Q, Cattle, Epigenesis
Abstract

Worldwide over 5 million children have been conceived using assisted reproductive technology, and research has concentrated on increasing the likelihood of ongoing pregnancy. However, studies using animal models have indicated undesirable effects of in vitro embryo culture on offspring development and health. In vivo, the oviduct hosts a period in which the early embryo undergoes complete reprogramming of its (epi)genome in preparation for the reacquisition of (epi)genetic marks. We designed an oviduct-on-a-chip platform to better investigate the mechanisms related to (epi)genetic reprogramming and the degree to which they differ between in vitro and in vivo embryos. The device supports more physiological (in vivo-like) zygote genetic reprogramming than conventional IVF. This approach will be instrumental in identifying and investigating factors critical to fertilization and pre-implantation development, which could improve the quality and (epi)genetic integrity of IVF zygotes with likely relevance for early embryonic and later fetal development., In vitro culture has detrimental effects on transcriptomes and epigenetic programming of zygotes. Here the authors use microfluidic technology to co-culture bovine oviduct epithelial cells with zygotes and show that the transcriptomes and global methylation patterns of these zygotes are more similar to in vivo zygotes than to conventionally cultured zygotes.

Published
2018

10. Multiwell capillarity-based microfluidic device for the study of 3D tumour tissue-2D endothelium interactions and drug screening in co-culture models

Author

Virumbrales-Muñoz, María, primary, Ayuso, José María, additional, Olave, Marta, additional, Monge, Rosa, additional, de Miguel, Diego, additional, Martínez-Lostao, Luis, additional, Le Gac, Séverine, additional, Doblare, Manuel, additional, Ochoa, Ignacio, additional, and Fernandez, Luis J., additional

Published
2017
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13. Progress and future of in vitro models to study translocation of nanoparticles

Author

Braakhuis, Hedwig M., primary, Kloet, Samantha K., additional, Kezic, Sanja, additional, Kuper, Frieke, additional, Park, Margriet V. D. Z., additional, Bellmann, Susann, additional, van der Zande, Meike, additional, Le Gac, Séverine, additional, Krystek, Petra, additional, Peters, Ruud J. B., additional, Rietjens, Ivonne M. C. M., additional, and Bouwmeester, Hans, additional

Published
2015
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