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16 results on '"Lin, Junfang"'

3. Characterization of nitrate assimilation in Lactuca sativa L. under different nitrogen sources

Author

Jie Pang, Lin Junfang, Lin Yizhang, Wang Shubin, Fenglin Zhong, Shuang Wu, Su-Feng Roan, Zhou Xiangzhu, Iou-Zen Chen, and Lin Biying

Subjects
inorganic chemicals, 0106 biological sciences, 0301 basic medicine, Physiology, Nitrogen assimilation, Lactuca, Plant Science, Reductase, engineering.material, Nitrate reductase, 01 natural sciences, 03 medical and health sciences, chemistry.chemical_compound, Nitrate, Nitrite, biology, organic chemicals, food and beverages, biology.organism_classification, Nitrite reductase, Horticulture, 030104 developmental biology, chemistry, engineering, Fertilizer, Agronomy and Crop Science, 010606 plant biology & botany
Abstract

Optimal fertilization management is critical for romaine lettuce (Lactuca dolichophylla K.) cultivation, a major economical vegetable in China. Herein, we chose a romaine lettuce genotype (Lactuca sativa L.) known for “year-round production” and as “high-yielding,” to examine the effect of varied nitrogen (N) fertilizer forms on nitrate (NO3−) accumulation. We cloned NO3− reductase (NR) and nitrite (NO2−) reductase (NiR) of romaine lettuce and analyzed their expressions at different growth stages in response to different N forms. Five N treatments with varying NO3−-N:NH4+-N ratios were examined. Our results showed that lettuce leaf NO3− content decreased significantly with the increase of NH4+-N in fertilizer. The lowest NO3− content was observed when NO3−-N:NH4+-N ratio was 0:100. NR and NiR activity and expression initially were upregulated by the increase in NO3− content, with the highest level at 70:30 NO3−-N:NH4+-N ratio, but quickly declined thereafter. We also found the temporal activation pattern of N transporters was different from that of NO3− reductase and NO2− reductase, suggesting there is a coordination between N translocation and N reduction in lettuce leaves to maintain proper N homeostasis. These findings provide insights into the role of NO3−-N:NH4+-N ratio in regulating NO3− assimilation, balancing N metabolism, and reducing NO2− toxicity in vegetable production.

Published
2018
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