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28 results on '"Liu, Yunjun"'

5. Chromosome-level genome assembly of a regenerable maize inbred line A188

Author

Lin, Guifang, primary, He, Cheng, additional, Zheng, Jun, additional, Koo, Dal-Hoe, additional, Le, Ha, additional, Zheng, Huakun, additional, Tamang, Tej Man, additional, Lin, Jinguang, additional, Liu, Yan, additional, Zhao, Mingxia, additional, Hao, Yangfan, additional, McFraland, Frank, additional, Wang, Bo, additional, Qin, Yang, additional, Tang, Haibao, additional, McCarty, Donald R., additional, Wei, Hairong, additional, Cho, Myeong-Je, additional, Park, Sunghun, additional, Kaeppler, Heidi, additional, Kaeppler, Shawn M., additional, Liu, Yunjun, additional, Springer, Nathan, additional, Schnable, Patrick S., additional, Wang, Guoying, additional, White, Frank F., additional, and Liu, Sanzhen, additional

Published
2021
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8. Development of monoclonal antibody-based sensitive ELISA for the determination of Cry1Ie protein in transgenic plant

Author

Liu Yunjun, Yan Liu, Jianhua Wang, Yuwen Zhang, Wei Zhang, and Wang Guoying

Subjects
0301 basic medicine, medicine.drug_class, Bacillus thuringiensis, Enzyme-Linked Immunosorbent Assay, Genetically modified crops, medicine.disease_cause, Monoclonal antibody, Zea mays, 01 natural sciences, Biochemistry, Analytical Chemistry, Hemolysin Proteins, 03 medical and health sciences, Bacterial Proteins, Escherichia coli, medicine, Bioassay, Genetically modified maize, Bacillus thuringiensis Toxins, biology, Chemistry, 010401 analytical chemistry, Antibodies, Monoclonal, Plants, Genetically Modified, biology.organism_classification, Molecular biology, 0104 chemical sciences, Endotoxins, 030104 developmental biology, Cry1Ac, Polyclonal antibodies, biology.protein
Abstract

Cry1Ie is a kind of Bacillus thuringiensis (Bt) toxin protein which has a different action model than the Cry1Ab and Cry1Ac protein. The transgenic maize expressing Cry1Ie might be commercially used in the near future and it is urgent to develop a method to detect Cry1Ie protein in transgenic plants and their products. To develop an ELISA method, Cry1Ie protein was expressed in Escherichia coli strain Transetta DE3, purified with the Ni-NTA spin columns, and then validated by sequencing. Bioassay results showed that the purified Cry1Ie protein was highly toxic to the Asian corn borer. The polyclonal antibody (pAb) and the specific monoclonal antibody (mAb) 1G42D6 were generated from rabbit and mice which were immunized with Cry1Ie protein, respectively. Western blotting of crude Cry1Ie protein extracts was established by employing mAb 1G42D6, whereas the mAb 1G42D6 negligibly recognized other Bt proteins. Sandwich ELISA against Cry1Ie protein was established by coating with pAb and detecting with mAb 1G42D6. The limit of detection (LOD), the limit of quantification (LOQ), and the quantification range of the assay in different matrices of maize plant were determined as 0.27–0.51, 0.29–0.78, and 0.45–15.71 ng/mL, respectively. Recoveries of Cry1Ie protein spiked in different maize tissues ranged from 75.1 to 99.5 %. The established sandwich ELISA was verified using transgenic maize overexpressing Cry1Ie. The results in this study suggested that the established ELISA method is effective for detecting Cry1Ie protein in transgenic plants.

Published
2016

10. Transgenic tobacco simultaneously overexpressing glyphosate N-acetyltransferase and 5-enolpyruvylshikimate-3-phosphate synthase are more resistant to glyphosate than those containing one gene

Author

Shengxue Zhang, Yuan Ren, Wei Lu, Guoying Wang, Jianhua Wang, Liu Yunjun, Rongrong Chen, and Gaoyi Cao

Subjects
Transgene, Drug Resistance, Glycine, Genetically modified crops, Polymerase Chain Reaction, chemistry.chemical_compound, Acetyltransferases, Tobacco, Botany, Genetics, Shikimate pathway, Gene, biology, ATP synthase, Herbicides, Aroa, fungi, food and beverages, Metabolism, Plants, Genetically Modified, biology.organism_classification, Molecular biology, Plant Leaves, chemistry, Glyphosate, biology.protein, Animal Science and Zoology, 3-Phosphoshikimate 1-Carboxyvinyltransferase, Agronomy and Crop Science, Biotechnology
Abstract

5-Enolpyruvylshikimate-3-phosphate synthase (EPSPS) and glyphosate N-acetyltransferase (GAT) can detoxify glyphosate by alleviating the suppression of shikimate pathway. In this study, we obtained transgenic tobacco plants overexpressing AM79 aroA, GAT, and both of them, respectively, to evaluate whether overexpression of both genes could confer transgenic plants with higher glyphosate resistance. The transgenic plants harboring GAT or AM79 aroA, respectively, showed good glyphosate resistance. As expected, the hybrid plants containing both GAT and AM79 aroA exhibited improved glyphosate resistance than the transgenic plants overexpressing only a single gene. When grown on media with high concentration of glyphosate, seedlings containing a single gene were severely inhibited, whereas plants expressing both genes were affected less. When transgenic plants grown in the greenhouse were sprayed with glyphosate, less damage was observed for the plants containing both genes. Metabolomics analysis showed that transgenic plants containing two genes could maintain the metabolism balance better than those containing one gene after glyphosate treatment. Glyphosate treatment did not lead to a huge increase of shikimate contents of tobacco leaves in transgenic plants overexpressing two genes, whereas significant increase of shikimate contents in transgenic plants containing only a single gene was observed. These results demonstrated that pyramiding both aroA and GAT in transgenic plants can enhance glyphosate resistance, and this strategy can be used for the development of transgenic glyphosate-resistant crops.

Published
2015

11. Metabolic effects of glyphosate on transgenic maize expressing a G2-EPSPS gene from Pseudomonas fluorescens

Author

Wang Guoying, Liu Yunjun, Liu Yan, Wei Lu, and Yuwen Zhang

Subjects
Genetically modified maize, biology, Metabolite, Transgene, Wild type, Glyoxylate cycle, Pseudomonas fluorescens, Plant Science, biology.organism_classification, Trehalose, chemistry.chemical_compound, Biochemistry, chemistry, Glyphosate, Botany, Agronomy and Crop Science, Biotechnology
Abstract

Transgenic glyphosate-tolerant maize expressing 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene has been commercialized since 1996. However, it is not very clear how glyphosate treatment affects metabolite pathway in transgenic glyphosate-tolerant maize. Here, we obtained numerous of glyphosate-tolerant transgenic maize expressing a Pseudomonas fluorescens G2-EPSPS gene. The expression and integration site of G2-EPSPS in transgenic maize event Aro203, which can tolerate 3 folds of field usage of glyphosate, were investigated. Metabolite analysis was performed with Aro203 leaf samples using GC/MS method. The results showed that total 58 metabolites were identified. Over-expression of G2-EPSPS led to the increase of glutamate, malate, hydroxylamine and trehalose contents, but the decrease of glyoxylate, ribose and sucrose, compared to wild type plants. Twenty-two and 13 metabolites were up-regulated and down-regulated in non-transgenic maize by glyphosate treatments, respectively, whereas fewer metabolites (10 up-regulated and 4 down-regulated) were affected in transgenic maize. Glyphosate treatment significantly stimulated the accumulation of most amino acids but decreased lots of sugars in non-transgenic plants. The PCA analysis results showed that wild type plant cluster treated with glyphosate was clearly separated with other three clusters. The results in this study provide evidence to understand how genetic modification or glyphosate treatment affects the metabolite pathway in maize.

Published
2014

25. Genome-wide association study dissects the genetic architecture of oil biosynthesis in maize kernels

Author

Li, Hui, primary, Peng, Zhiyu, additional, Yang, Xiaohong, additional, Wang, Weidong, additional, Fu, Junjie, additional, Wang, Jianhua, additional, Han, Yingjia, additional, Chai, Yuchao, additional, Guo, Tingting, additional, Yang, Ning, additional, Liu, Jie, additional, Warburton, Marilyn L, additional, Cheng, Yanbing, additional, Hao, Xiaomin, additional, Zhang, Pan, additional, Zhao, Jinyang, additional, Liu, Yunjun, additional, Wang, Guoying, additional, Li, Jiansheng, additional, and Yan, Jianbing, additional

Published
2012
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