1. Chromosomal localization of foreign genes in Petunia hybrida
- Author
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Stephen G. Rogers, Robert B. Horsch, Robert T. Fraley, Marco Wallroth, and Anton G. M. Gerats
- Subjects
Genetics ,Transformation (genetics) ,Gene mapping ,Genetic transfer ,Chimeric gene ,Agrobacterium tumefaciens ,Biology ,biology.organism_classification ,Molecular Biology ,Petunia ,Gene ,Selectable marker - Abstract
A F1 hybrid of Petunia hybrida, heterozygous for at least one marker on each of the seven chromosomes, was transformed with a modified strain of Agrobacterium tumefaciens in which the phytohormone biosynthetic genes in the transferred DNA (T-DNA) were replaced with a NOS/NPTII/NOS chimeric gene and a wildtype nopaline synthase (NOS) gene. The chimeric gene, which confers kanamycin resistance, was used as selectable marker during the transformation process and the NOS gene was used as a scorable marker in the genetic studies. After plants had been regenerated from the transformed tissues, the transgenic plants that expressed both of these markers were backcrossed to the parental lines. The offspring were examined for the segregation of the NOS gene and the Petunia markers. Genetic mapping was thus accomplished in a single generation.
- Published
- 1986
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