1. Plasmodium falciparum genetic crosses in a humanized mouse model
- Author
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Tim J. Anderson, Michael T. Ferdig, Lisa A. Checkley, Nelly Camargo, Richard S. Pinapati, Stefan H. I. Kappe, Shalini Nair, Matthew Fishbaugher, Carolyn A. Hutyra, Ashley M. Vaughan, François Nosten, and Ian H. Cheeseman
- Subjects
Plasmodium falciparum ,030231 tropical medicine ,Drug Resistance ,Biology ,Biochemistry ,Article ,law.invention ,Mice ,03 medical and health sciences ,0302 clinical medicine ,law ,parasitic diseases ,medicine ,Animals ,Humans ,Parasite hosting ,Molecular Biology ,Gene ,Crosses, Genetic ,030304 developmental biology ,Genetics ,0303 health sciences ,Cell Biology ,medicine.disease ,biology.organism_classification ,Phenotype ,Artemisinins ,3. Good health ,Parasitology ,Humanized mouse ,Recombinant DNA ,Malaria ,Biotechnology - Abstract
Genetic crosses of phenotypically distinct strains of the human malaria parasite Plasmodium falciparum are a powerful tool for identifying genes controlling drug resistance and other key phenotypes. Previous studies relied on the isolation of recombinant parasites from splenectomized chimpanzees, a research avenue that is no longer available. Here, we demonstrate that human-liver chimeric mice support recovery of recombinant progeny for the identification of genetic determinants of parasite traits and adaptations.
- Published
- 2015
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