1. Co-inoculation of Lolium perenne with Funneliformis mosseae and the dark septate endophyte Cadophora sp. in a trace element-polluted soil
- Author
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Corinne Leyval, Damien Blaudez, Charlotte Berthelot, Michel Chalot, Thierry Beguiristain, Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Terre et Environnement de Lorraine (OTELo), Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), and Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)
- Subjects
0106 biological sciences ,0301 basic medicine ,Arbuscular mycorrhizal fungi ,Plant Science ,Dark septate endophyte ,01 natural sciences ,Lolium perenne ,Glomeromycota ,Cadophora sp ,03 medical and health sciences ,Ascomycota ,Mycorrhizae ,Endophytes ,Lolium ,Genetics ,Soil Pollutants ,Molecular Biology ,Soil Microbiology ,[SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology ,Ecology, Evolution, Behavior and Systematics ,2. Zero hunger ,Rhizosphere ,biology ,Inoculation ,fungi ,food and beverages ,General Medicine ,[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics ,15. Life on land ,biology.organism_classification ,Heavy metal pollution ,Trace Elements ,Horticulture ,030104 developmental biology ,Funneliformis mosseae ,Shoot ,Co-inoculation ,France ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,Soil microbiology ,[SDV.EE.IEO]Life Sciences [q-bio]/Ecology, environment/Symbiosis ,010606 plant biology & botany - Abstract
International audience; The presence of dark septate endophytes (DSEs) or arbuscular mycorrhizal fungi (AMF) in plant roots and their effects on plant fitness have been extensively described. However, little is known about their interactions when they are simultaneously colonizing a plant root, especially in trace element (TE)-polluted soils. We therefore investigated the effects of Cadophora sp. and Funneliformis mosseae on ryegrass (Lolium perenne) growth and element uptake in a Cd/Zn/Pb-polluted soil. The experiment included four treatments, i.e., inoculation with Cadophora sp., inoculation with F. mosseae, co-inoculation with Cadophora sp. and F. mosseae, and no inoculation. Ryegrass biomass and shoot Na, P, K, and Mg concentrations significantly increased following AMF inoculation as compared to non-inoculated controls. Similarly, DSE inoculation increased shoot Na concentration, whereas dual inoculation significantly decreased shoot Cd concentration. Moreover, oxidative stress determined by ryegrass leaf malondialdehyde concentration was alleviated both in the AMF and dual inoculation treatments. We used quantitative PCR and microscope observations to quantify colonization rates. They demonstrated that DSEs had no effect on AMF colonization, while AMF colonization slightly decreased DSE frequency. We also monitored fluorescein diacetate (FDA) hydrolysis and alkaline phosphatase (AP) activity in the rhizosphere soils. FDA hydrolysis remained unchanged in the three inoculated treatments, but AMF colonization increased AP activity and P mobility in the soil whereas DSE colonization did not alter AP activity. In this experiment, we unveiled the interactions between two ecologically important fungal groups likely to occur in roots which involved a decrease of oxidative stress and Cd accumulation in shoots. These results open promising perspectives on the fungal-based phytomanagement of TE-contaminated sites by the production of uncontaminated and marketable plant biomass.
- Published
- 2018
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