Search

This study is a proof of concept performed to evaluate process parameters affecting shape memory effect of copolymer poly-l-lactide-co-poly-ε-caprolactone (PLA:PCL) 70:30 ratio based nanofibrous scaffolds. A design of experiment (DOE) statistical approach was used to define the interaction between independent material and process variables related to electrospun scaffold manufacturing, such as polymer solution concentration (w/v%), spinning time (min), and needle size (Gauge), and their influence on Rf% (ability of the scaffold to maintain the induced temporary shape) and Rr% (ability of the scaffold to recover its original shape) outputs. A mathematical model was obtained from DOE useful to predict scaffold Rf% and Rr% values. PLA-PCL 15% w/v, 22G needle, and 20-min spinning time were selected to confirm the data obtained from theoretical model. Subsequent morphological (SEM), chemical-physical (GPC and DSC), mechanical (uniaxial tensile tests), and biological (cell viability and adhesion) characterizations were performed. Graphical abstract

In recent decades, public policies of the Unified Health System (SUS) in Brazil have structured a community mental health care network (RAPS) based on various community actions and services. This study carried out evaluative research on the implementation of the structure and process dimensions of this care network in Minas Gerais, the second most populous state of Brazil, generating indicators that can enhance the strategic management of the public health system in the strengthening the psychosocial care in the state. The application of a multidimensional instrument, previously validated (IMAI-RAPS), in 795 of the 853 municipalities in Minas Gerais was carried out between June and August 2020. Regarding the structural dimension, we noticed an adequate implementation of services like ‘Family Health Strategy,’ ‘Expanded Family Health Center,’ and ‘Psychosocial Care Centers’ but a lack of ‘Beds in General Hospitals’ destinated to mental health care, ‘Unified Electronic Medical Records’ and ‘Mental Health Training Activities for Professionals.’ In the process dimension, adequate implementation of actions such as ‘Multidisciplinary and Joint Care,’ ‘Assistance to Common Mental Disorders by Primary Health Care,’ ‘Management of Psychiatric Crises in Psychosocial Care Centers,’ ‘Offer of Health Promotion Actions,’ and ‘Discussion of Cases by Mental Health Teams’ point to a form of work consistent with the guidelines. However, we detected difficulties in the implementation of ‘Psychosocial Rehabilitation Actions,’ ‘Productive Inclusion,’ ‘User Protagonism,’ ‘Network Integration,’ and practical activities for the effectiveness of collaborative care. We found a better implementation of the mental health care network in more populous, demographically dense, and socioeconomically developed cities, which shows the importance of regional sharing of services that are not possible for small cities. The evaluation practices of mental health care networks are scarce throughout the Brazilian territory, a fact also found in Minas Gerais, highlighting the need for its expansion not only in the scientific sphere but also in the daily life of the various levels of management.

This executive document reflects and updates the key points of a Consensus document on Cardiovascular (CV) Prevention realized through the contribution of a number of Italian Scientific Societies and coordinated by the Italian Society of Cardiovascular Prevention (SIPREC). The aim of this executive document is to analyze and discuss the new recommendations introduced by international guidelines for the management of major CV risk factors, such as hypertension, dyslipidemias and type 2 diabetes, consisting in the identification of lower therapeutic targets, in the promotion of combination fixed drug therapies and in the introduction in routine clinical practice of new effective pharmacological classes. Moreover, the document highlights the importance of effective CV prevention strategies during the the coronavirus disease 2019 (COVID-19) outbreak which has dramatically changed the priorities and the use of available resources by the national healthcare systems and have caused a reduction of programmed follow-up visits and procedures and even of hospital admissions for severe acute pathologies. In addition, the pandemic and the consequent lockdown measures imposed have caused a widespread diffusion of unhealthy behaviors with detrimental effects on the CV system. In such a context, reinforcement of CV prevention activities may play a key role in reducing the future impact of these deleterious conditions.

The nucleocapsid (N) protein plays critical roles in coronavirus genome transcription and packaging, representing a key target for the development of novel antivirals, and for which structural information on ligand binding is scarce. We used a novel fluorescence polarization assay to identify small molecules that disrupt the binding of the N protein to a target RNA derived from the SARS-CoV-2 genome packaging signal. Several phenolic compounds, including L-chicoric acid (CA), were identified as high-affinity N-protein ligands. The binding of CA to the N protein was confirmed by isothermal titration calorimetry, 1H-STD and 15N-HSQC NMR, and by the crystal structure of CA bound to the N protein C-terminal domain (CTD), further revealing a new modulatory site in the SARS-CoV-2 N protein. Moreover, CA reduced SARS-CoV-2 replication in cell cultures. These data thus open venues for the development of new antivirals targeting the N protein, an essential and yet underexplored coronavirus target.

As 3D in vitro tissue models become more pervasive, their built-in nutrient, metabolite, compound, and waste gradients increase biological relevance at the cost of analysis simplicity. Investigating these gradients and the resulting metabolic heterogeneity requires invasive and time-consuming methods. An alternative is using electrochemical biosensors and measuring concentrations around the tissue model to obtain size-dependent metabolism data. With our hanging-drop-integrated enzymatic glucose biosensors, we conducted current measurements within hanging-drop compartments hosting spheroids formed from the human colorectal carcinoma cell line HCT116. We developed a physics-based mathematical model of analyte consumption and transport, considering (1) diffusion and enzymatic conversion of glucose to form hydrogen peroxide (H2O2) by the glucose-oxidase-based hydrogel functionalization of our biosensors at the microscale; (2) H2O2 oxidation at the electrode surface, leading to amperometric H2O2 readout; (3) glucose diffusion and glucose consumption by cancer cells in a spherical tissue model at the microscale; (4) glucose and H2O2 transport in our hanging-drop compartments at the macroscale; and (5) solvent evaporation, leading to glucose and H2O2 upconcentration. Our model relates the measured currents to the glucose concentrations generating the currents. The low limit of detection of our biosensors (0.4 ± 0.1 μM), combined with our current-fitting method, enabled us to reveal glucose dynamics within our system. By measuring glucose dynamics in hanging-drop compartments populated by cancer spheroids of various sizes, we could infer glucose distributions within the spheroid, which will help translate in vitro 3D tissue model results to in vivo., Microsystems & Nanoengineering, 8 (1), ISSN:2096-1030, ISSN:2055-7434

Purpose To analyse the influence of goalkeepers during 4-a-side small-sided games, played in pitches of two different sizes (small: 30 × 20 m, large: 40 × 30 m). Methods Total distance covered (TD), distance covered at low- (LSD), moderate- (MSD), high- (HSD) and very high-speed (VHSD), average and maximal speed, Edwards’ training load (Edw-TL), time spent above 90% of maximal heart rate (T90%) and rate of perceived exertion (RPE) were monitored, in 18 amateur soccer players. Results Higher TD (mean difference: + 181 m, Hedge’s g: 0.93 and + 400 m, 3.37), MSD (+ 85 m, 0.79 and + 146 m, 1.64), HSD (+ 101 m, 1.41 and + 179 m, 3.26), VHSD (+ 30 m, 1.89 and + 35 m, 1.26), average speed (+ 0.65 km h−1, 0.88 and + 1.47 km h−1, 3.31) and maximal speed (+ 3.60 km h−1, 1.40 and + 3.58 km h−1, 1.40) were found in large than small pitch, without and with goalkeepers, respectively. Goalkeeper’s presence increased Edw-TL (+ 8.4 AU, 0.70) and reduced TD (− 141 m, 0.75), HSD (− 54 m, 0.75) and average speed (− 0.54 km h−1, 0.76) in small pitch and maximal speed (1.59 km h−1, 0.60 and 1.61 km h−1, 0.66) in both small and large pitches, respectively. RPE was higher (+ 20, 1.52) in the large than small pitch when the goalkeepers were present. Conclusion Implementing small-sided games, coaches should be aware that lower external load with similar internal load could be provided using small pitch with goalkeeper rather than either small goals or larger pitch. Furthermore, large small-sided games without goalkeeper may be the best choice for eliciting high training load.

The Coping Power Program is an evidence-based intervention for children identified to be at risk for developing externalizing behavioral problems. The Coping Power Program has been adapted to universally prevent behavioral problems in school-aged children. This study sought to test the efficacy of this adaptation, the Coping Power Universal program, on preschoolers' behavioral difficulties and pre-academic skills. Teachers delivered the intervention in their classes. The study included a sample of Italian children (N = 250, 125 boys) with a mean age of 4.50 years (SD = .50) at the beginning of the study. Classrooms of these children were randomly assigned to receive either the intervention or the Italian preschool standard curriculum. Measures included a questionnaire and objective and standardized measures for numerical intelligence and metaphonological skills delivered by a psychologist to the preschoolers. Classes in which teachers applied the Coping Power Universal program showed lower problematic behaviors and higher pre-academic skills than those in which teachers followed the standard curriculum only. Although further studies are still needed, the current findings showed that the Coping Power Universal program can be adapted for preschoolers with good results. Implications for practice, methodological limitations, and directions for future research are reviewed.

Non-ablative and ablative fractional erbium lasers are among the most frequently used resources in dermatology for facial rejuvenation and for treating dermatological disorders. This type of erbium laser can be found at wavelengths of 1540 or 1550 nm, which are classified as non-ablative erbium glass, and at 2940 nm, classified as ablative erbium YAG. Despite the reports of their clinical benefits, few scientific studies have demonstrated the efficacy and safety of these lasers in the short or long term. In order to substantiate the effects, benefits, and safety of applying the erbium glass and erbium YAG lasers, a systematic review was carried out from August to December 2019 about studies published in the last 20 years. Randomized clinical trials in humans were considered that evaluated the efficacy, safety, and benefits of applying the fractional lasers erbium glass and erbium YAG to facial rejuvenation, skin spots, and atrophic acne scars. A total of 338 articles were identified; 76 articles remained after their titles and abstracts were read, and 42 articles were selected after removing the duplicates. After the articles were read in full, 17 of these articles were included in the systematic review (453 patients). The erbium glass and erbium YAG lasers seem promising in the short term, with minimal adverse effects; however, the long-term efficacy and safety still present limitations. Consequently, future research is needed, with better methodological standardization and a follow-up with a longer evaluation period for possible permanent adverse effects to determine the standardization and safety of therapy with erbium glass and erbium YAG lasers.

Using light sources in phototherapy has presented promising results regarding several types of facial and body skin affections for centuries. The neodymium-doped yttrium aluminum garnet (ND:YAG) 1064 nm laser has become a standard treatment in dermatology for dermatological disturbances related to the pilous follicle and the structures surrounding it. Despite the reports of its clinical benefits, few scientific studies show its actual safety and efficacy. To substantiate the effects, benefits, and safety of applying an ND:YAG 1064 laser in the epilation process during esthetic treatment, a systematic review was carried out between October 2018 and January 2019, on studies published in the last 20 years. Randomized clinical trials in humans were considered that evaluated the efficacy, safety, and benefits of ND:YAG laser application at 1064 nm wavelengths for hair removal. A total of 198 articles were identified; 58 articles remained after their titles and abstracts were read; and 21 articles were selected after the removal of duplicates. After the integral reading, 13 of these articles were included in the systematic review (734 patients). The ND:YAG 1064 nm laser presents safety and promising, beneficial effects in epilation. However, further scientific studies with better methodologic quality are necessary to better standardize ND:YAG laser therapy and clarify its parameters for photoepilation.

In a joint work with S. Bianchini [8] (see also [6, 7]), we proved a quadratic interaction estimate for the system of conservation laws $$ \left\{\begin{array}{l}{u}_t+f{(u)}_x=0,\\ {}u\left(t=0\right)={u}_0(x),\end{array}\right. $$ where u : [0, ∞) × ℝ → ℝn, f : ℝn → ℝn is strictly hyperbolic, and Tot.Var.(u0) ≪ 1. For a wavefront solution in which only two wavefronts at a time interact, such an estimate can be written in the form $$ \sum \limits_{t_j\;\mathrm{interaction}\ \mathrm{time}}\frac{\left|\sigma \left({\alpha}_j\right)-\sigma \left({\alpha}_j^{\prime}\right)\right|\left|{\alpha}_j\right|\left|{\alpha}_j^{\prime}\right|}{\left|{\alpha}_j\right|+\left|{\alpha}_j^{\prime}\right|}\le C(f)\mathrm{Tot}.\mathrm{Var}.{\left({u}_0\right)}^2, $$ where αj and $$ {\alpha}_j^{\prime } $$ are the wavefronts interacting at the interaction time tj, σ(·) is the speed, |·| denotes the strength, and C(f) is a constant depending only on f (see [8, Theorem 1.1] or Theorem 3.1 in the present paper for a more general form). The aim of this paper is to provide the reader with a proof for such a quadratic estimate in a simplified setting, in which: • all the main ideas of the construction are presented; • all the technicalities of the proof in the general setting [8] are avoided.

The purpose of a prosthetic implant is to reproduce anatomy as normal as possible and properly functioning, to correct any defects that may have caused joint degeneration or failure of a previously placed implant. Ideal prostheses, in fact, should not only replace an articulation but must be integrated into the complicated load transfer system from the trunk to the limbs. The causes of prosthetic implant failure are several but in up to 77% of cases responsibility can be attributed to a biomechanical problem. To give longer life to our implant, it is therefore necessary to know and apply rules for the recovery of biomechanical parameters. At the disposal of the surgeon in the preoperative phase there are lots of investigation, tools and applications that prevent possible complications, predict the positioning of the implant in all its components so that it can also handle the surgical options available with surgery. Accurate planning with the use of current technologies will help to minimise potential implant-related problems. The essence of preoperative planning in hip surgery can simply be summed in two words: be prepared.

Orthotropic steel decks, directly subjected to traffic loads, are very sensitive to fatigue: defects mainly appear as cracks, which affect the top plates, longitudinal ribs and cross-beams of the deck. In this kind of bridges, structural health monitoring can be effectively adopted in characterising the safety threshold of the ongoing fatigue damage. This paper estimates the residual fatigue life of an existing orthotropic box-girder bridge, using experimental data from long-term monitoring of real traffic-induced effects. The bridge in question, less than 25 years old, is located on one of Italy’s busiest toll highways and evidenced fatigue damage due to deficiencies in original design and detailing. After preliminary visual inspections, the dynamic identification of the bridge was investigated by operational modal analysis, and a long-term monitoring campaign of strain measurements was subsequently carried out, involving series of strain gauges located on the most critical bridge sections. According to stress cycles recorded at these points, unlimited life verification was carried out according to EN 1993-1-9, and estimates of the residual fatigue life of the various elements were then derived by extrapolation of the experimental data. The proposed method can effectively be applied to fatigued steel bridge decks so that residual fatigue life and the real urgency of retrofitting interventions can be evaluated in operational conditions.

The role of genetic molecular markers in neoadjuvant treatment for locally advanced esophageal cancer has been reviewed, focusing strictly on concurrent chemoradiation protocols followed by surgery. Eleven studies evaluated the role of mRNA expression profile; the end point was overall survival (OS) in two studies and different definitions of histological response in nine. Genes reported as significant were involved in cell cycle control (30), apoptosis (7), structural molecules (9), cell metabolism (6) and DNA repair (1). Seven studies reported about 15 microRNA (miRNA) molecules associated with OS (2) or histological response (13), however, defined with different classifications. Their target genes were prevalently involved in cell cycle control (4), apoptosis (1), cell adhesion (1), migration (1) and angiogenesis (1). Gene polymorphisms (single-nucleotide polymorphisms (SNPs)) have been evaluated in 8 studies reporting 10 variants associated with survival or pathological response. OS was the end point in six of these studies. SNPs reported as significant were involved in DNA repair system (4), detoxification (2), folate metabolism (6), drug efflux (2) and others (2). In a study, a panel including histology, pathological response and five SNPs discriminated two subsets of patients with 5-year survival rates of 79.3% and 26.3% (hazard ratio 6.25, P

Wastewater treatment plants use many sensors to control energy consumption and discharge quality. These sensors produce a vast amount of data which can be efficiently monitored by automatic systems. Consequently, several different statistical and learning methods are proposed in the literature which can automatically detect faults. While these methods have shown promising results, the nonlinear dynamics and complex interactions of the variables in wastewater data necessitate more powerful methods with higher learning capacities. In response, this study focusses on modelling faults in the oxidation and nitrification process. Specifically, this study investigates a method based on deep neural networks (specifically, long short-term memory) compared with statistical and traditional machine-learning methods. The network is specifically designed to capture temporal behaviour of sensor data. The proposed method is evaluated on a real-life dataset containing over 5.1 million sensor data points. The method achieved a fault detection rate (recall) of over 92%, thus outperforming traditional methods and enabling timely detection of collective faults.

This paper deals with the dynamic response of a free-standing ancient column in the Roman Agora of Thessaloniki, Greece as a means to shed more light on the complex behaviour of rocking bodies under seismic excitation. Numerical analyses utilizing discrete element method were carried out with the use of multiple seismic records selected based on the disaggregation of the seismic hazard for the region of interest. To identify their impact on structural performance, earthquake Intensity Measures, such as Peak Ground Acceleration and Peak Ground Velocity are examined for the case of a column that sustained no visible permanent deformations during the Ms = 6.5 Thessaloniki earthquake of 1978. The analysis revealed a weak correlation of PGA and PGV with the response results and a significant influence of the mean frequency (fm) of the seismic motion. No coupling was found between the maximum displacement of the top during the oscillation and the permanent post-seismic deformations. The complementarity of both earthquake Intensity Measures in the structural vulnerability assessment is also depicted.

Despite the development of novel effective therapeutic strategies, metastatic castration-resistant prostate cancer (mCRPC) remains a disease with a lethal course and a high biological and molecular heterogeneity. To date, germline mutations in the BRCA gene represent one of the main risk factors for developing prostate cancer, with a strong association with aggressive phenotype and poor clinical outcomes. A better understanding of the genomic landscape of prostate cancer has strengthened the idea that "synthetic lethality" of this disease might be useful in cancer-drug discovery, focusing on agents such as platinum compounds and poly (adenosine diphosphate [ADP]-ribose) polymerase inhibitors (PARPi). In this review, we summarize the main data available on BRCA mutations and discuss the clinical implications of these genomic aberrations in the management of prostate cancer, stressing the need to identify prognostic and predictive biomarkers and to deeply understand the mechanisms of treatment resistance, in order to maximize personalized medicine protocols and therefore clinical benefit.

In the last decade the number of structural health monitoring (SHM) systems designed and implemented on historic structures increased exponentially. Monitoring perfectly meets principles and guidelines of the recently issued Italian and European seismic codes as it is considered a strategic activity in the knowledge process of historic buildings, in order to understand their structural behavior and have a deeper insight on their health conditions. Thanks to SHM it is possible to intervene on buildings with more confidence (and only when necessary), but also to prevent the execution of intrusive strengthening interventions, in full compliance with the minimum intervention principle, so important in the restoration field. The object of monitoring is to identify, locate and classify type and severity of damages induced by external actions or degradation phenomena and to assess their effects on the structural performance. In this way it is possible to take appropriate measures to reduce the danger of collapse and, when necessary, perform strengthening interventions to improve the structural and seismic capacity. Motivated by the above reasons, the paper provide a contribution to the application of integrated methodologies and techniques, based on SHM, for the assessment and protection of cultural heritage (CH) buildings and existing structures. Selected case studies, equipped with distributed sensors and acquisition systems, allowed the definition and successive validation of SHM as a knowledge-based assessment tool, implemented to: (1) avoid the execution of unnecessary interventions and assess structural vulnerabilities; (2) evaluate intervention needs, following an incremental approach during their execution; (3) control the damage states of buildings in a post-seismic scenario. Monitoring data, automatically processed through dedicated software for static monitoring and automated algorithms for modal parameters extraction, are then used for the assessment of the health conditions and the identification of active damaging processes based on numerical simulations and robust statistical models.

In a series of joint works with S. Bianchini [3, 4, 5], we proved a quadratic interaction estimate for general systems of conservation laws. Aim of this paper is to present the results obtained in the three cited articles [3, 4, 5], discussing how they are related with the general theory of hyperbolic conservation laws. To this purpose, first we explain why this quadratic estimate is interesting, then we give a brief overview of the techniques we used to prove it and finally we present some related open problems.

Candidate genes involved in DNA repair, 5-fluorouracil metabolism and drug detoxification were genotyped in 124 patients receiving neoadjuvant chemoradiation treatment for locally advanced esophageal cancer and their predictive role for long-term relapse-free survival (RFS) and cancer-specific survival (CSS) were evaluated. A panel including MTHFR 677TT, MDR1 2677GT, GSTP1 114CC, XPC 499CC and XPC 939AC+CC, defined as high-risk genotypes, discriminated subgroups with significantly different outcomes. When the panel was combined with histology, patients split into two subsets with 5-year RFS and CSS rates of 65% vs 27% (hazard ratio (HR) 3.0, P

The increase of Zika virus (ZIKV) infections in Brazil in the last two years leaves a prophylactic measures on alert for this new and emerging pathogen. Concerning of our positive experience, we developed a new prototype using Neisseria meningitidis outer membrane vesicles (OMV) on ZIKV cell growth in a fusion of OMV in the envelope of virus particles. The fusion of nanoparticles resulting from outer membrane vesicles of N. meningitidis with infected C6/36 cells line were analyzed by Nano tracking analysis (NTA), zeta potential, differential light scattering (DLS), scan and scanning transmission eletronic microscopy (SEM and STEM) and high resolution mass spectometry (HRMS) for nanostructure characterization. Also, the vaccination effects were viewed by immune response in mice protocols immunization (ELISA and inflammatory chemokines) confirmed by Zika virus soroneutralization test. The results of immunizations in mice showed that antibody production had a titer greater than 1:160 as compared to unvaccinated mice. The immune response of the adjuvant and non-adjuvant formulation activated the cellular immune response TH1 and TH2. In addition, the serum neutralization was able to prevent infection of virus particles in the glial tumor cell model (M059J). This research shows efficient strategies without recombinant technology or DNA vaccines.