1. Estimation of the quantity of bacteria encapsulated in Lentikats Biocatalyst via phospholipid fatty acids content: a preliminary study
- Author
-
Lucie Křiklavová, Josef Trögl, Pavel Kuráň, Jana Pavlorková, Petra Zemánková, Ivana Jirková, Věra Pilařová, Jan Popelka, and Petra Veronesi Dáňová
- Subjects
Microbial Viability ,Denitrification ,Bacteria ,Drug Compounding ,Fatty Acids ,Phospholipid ,Biomass ,General Medicine ,Biology ,biology.organism_classification ,Microbiology ,Polyvinyl alcohol ,Bacterial Load ,chemistry.chemical_compound ,chemistry ,Biocatalysis ,Organic chemistry ,lipids (amino acids, peptides, and proteins) ,Food science ,Paracoccus denitrificans ,Phospholipids ,Biotechnology - Abstract
The content of phospholipid fatty acids (PLFA) was determined in samples of polyvinyl alcohol lenses (Lentikats Biocatalyst, LB) with encapsulated Paracoccus denitrificans withdrawn during long-term denitrification experiments. The total PLFA content correlated highly with specific denitrification activities of LB as well as biomass estimation based on image analyses of microscopic photos. The results confirmed the applicability of PLFA determination for estimation of the amount of living encapsulated microbial biomass during biotechnological applications.
- Published
- 2012
- Full Text
- View/download PDF