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31 results on '"Qiao, Mu"'

2. Genome-wide detection of selection signatures in Jianli pigs reveals novel cis-regulatory haplotype in EDNRB associated with two-end black coat color

Author

Xu, Zhong, primary, Wu, Junjing, additional, Zhang, Yu, additional, Qiao, Mu, additional, Zhou, Jiawei, additional, Feng, Yue, additional, Li, Zipeng, additional, Sun, Hua, additional, Lin, Ruiyi, additional, Song, Zhongxu, additional, Zhao, Haizhong, additional, Li, Lianghua, additional, Chen, Nanqi, additional, Li, Yujie, additional, Oyelami, Favour Oluwapelumi, additional, Peng, Xianwen, additional, and Mei, Shuqi, additional

Published
2024
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3. Evolution of neuronal cell classes and types in the vertebrate retina

Author

Hahn, Joshua, primary, Monavarfeshani, Aboozar, additional, Qiao, Mu, additional, Kao, Allison H., additional, Kölsch, Yvonne, additional, Kumar, Ayush, additional, Kunze, Vincent P., additional, Rasys, Ashley M., additional, Richardson, Rose, additional, Wekselblatt, Joseph B., additional, Baier, Herwig, additional, Lucas, Robert J., additional, Li, Wei, additional, Meister, Markus, additional, Trachtenberg, Joshua T., additional, Yan, Wenjun, additional, Peng, Yi-Rong, additional, Sanes, Joshua R., additional, and Shekhar, Karthik, additional

Published
2023
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6. Scalable and programmable phononic network with trapped ions

Author

Chen, Wentao, primary, Lu, Yao, additional, Zhang, Shuaining, additional, Zhang, Kuan, additional, Huang, Guanhao, additional, Qiao, Mu, additional, Su, Xiaolu, additional, Zhang, Jialiang, additional, Zhang, Jing-Ning, additional, Banchi, Leonardo, additional, Kim, M. S., additional, and Kim, Kihwan, additional

Published
2023
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14. Molecular cloning, characterization and evolutionary analysis of vitellogenin in Chinese giant salamander Andrias davidianus

Author

Hanbing Xiao, Meng Yan, Haifeng Tian, and Qiao-mu Hu

Subjects
Genetics, Amphibian, education.field_of_study, biology, Andrias, Population, Cell Biology, Plant Science, Chinese giant salamander, Molecular cloning, biology.organism_classification, Biochemistry, Homology (biology), Vitellogenin, biology.animal, Complementary DNA, biology.protein, Animal Science and Zoology, education, Molecular Biology, Ecology, Evolution, Behavior and Systematics
Abstract

Vitellogenin (VTG) is the major precursor of yolk proteins and could transport many components into oocytes, and hence it was thought to be a potential marker of reproduction of oviparious animal. Andrias davidianus is a critically endangered amphibian species, of which the wild population decreased dramatically and the artificial breeding has been successfully established. In this study, a full-length VTG cDNA was cloned and characterized from A. davidianus (designated as AdVTG) using 5′ and 3′ rapid amplification cDNA ends and RT-PCR. The AdVTG cDNA is 5,418 base pair in length and it encodes 1,805 amino acid long protein with typical quadripartite domains and several conserved cleavage sites. The tissue expression pattern indicated that AdVTG is mainly expressed in liver in one-year old individual. The three-dimensional model of AdVTG constructed by homology modelling revealed the presence of conserved N-terminal β-sheet and lipid-binding cavity. Moreover, the phylogenetic analysis of VTG in metazoa was carried out, which revealed that Anura-specific gene duplication occurred after the divergence of Caudata. All these results would improve the understanding of the physiological roles of AdVTG in A. davidianus and contribute to elucidating the evolution of VTG in vertebrates.

Published
2015
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17. Imprinting analysis of porcine MAGEL2 gene in two fetal stages and association analysis with carcass traits

Author

Qiao Mu, Chao Wang, Ling Guo, Chang-Yan Deng, Yuanzhu Xiong, and Rong Zheng

Subjects
Candidate gene, Meat, Molecular Sequence Data, Sus scrofa, Population, Single-nucleotide polymorphism, Breeding, Biology, Polymorphism, Single Nucleotide, Andrology, Genomic Imprinting, Open Reading Frames, Fetus, Genetics, Animals, Cloning, Molecular, Allele, Imprinting (psychology), education, Molecular Biology, Gene, Genetic Association Studies, education.field_of_study, Base Sequence, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, Sequence Analysis, DNA, General Medicine, Gene expression profiling, Body Composition, Genomic imprinting, Melanoma-Specific Antigens, Polymorphism, Restriction Fragment Length
Abstract

Imprinted genes play an essential role in the regulation of fetal growth, development and function of the placenta, however only a limited number of imprinted genes have been studied in swine. In this study, we cloned and characterized porcine MAGEL2 (melanoma antigen-like gene 2), and also identified its imprinting status during porcine fetal development. The complete open reading frame (ORF) encoding 1,193 amino acids was isolated and two single nucleotide polymorphisms (SNPs) (g.2592A>C and g.3277T>C) in the coding region were identified. The reciprocal Yorkshire × Meishan F1 hybrid model and the RT-PCR/RFLP method were used to detect the imprinting status of porcine MAGEL2 gene at two developmental stages of day 30 and 65 of gestation. Imprinting analysis showed that porcine MAGEL2 was paternally expressed in day 65 fetal tissues, including heart, liver, spleen, lung, kidney, stomach, small intestine, skeletal muscle, brain and placenta. Interestingly, we observed an imprinting variance of MAGEL2 gene in 30 dpc fetuses produced by the cross of Yorkshire boar × Meishan sow, in which seven heterozygous fetuses were monoallelically expressed from the paternal allele but two were biallelically expressed from both the paternal and maternal alleles. Association analysis in a Yorkshire × Meishan F2 resource population showed that the mutation of g.2592A>C was significantly associated with dressed carcass percentage (P < 0.05) and buttock fat thickness (P < 0.05). Our results suggest that MAGEL2, as a novel imprinted gene in pig, might be a candidate gene affecting carcass traits and could provide important information for the functional study of imprinted genes during porcine development.

Published
2011
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18. A hybrid promoter-containing vector for direct cloning and enhanced expression of PCR-amplified ORFs in mammalian cells

Author

Yun-chao Liu, Man Teng, Ya-nan Guo, Qiao-mu Li, Gaiping Zhang, Jun Luo, Aiping Wang, Haibo Weng, and Leiming You

Subjects
Erythrocytes, Genetic Vectors, Green Fluorescent Proteins, Molecular Sequence Data, Biology, Transfection, Polymerase Chain Reaction, Cell Line, Mice, Open Reading Frames, Genetics, Animals, Humans, Vector (molecular biology), Cloning, Molecular, ORFS, Promoter Regions, Genetic, Enhancer, Molecular Biology, Gene, Cloning, Expression vector, Base Sequence, Receptors, IgG, General Medicine, Molecular biology, Introns, TA cloning, Expression cassette, Plasmids
Abstract

An efficient vector, designated as pCAGX, was designed for direct cloning and enhanced expression of PCR-amplified ORFs in mammalian cells. It relied on the well-known TA-cloning principle, and utilized the CMV enhancer/chicken beta-actin/rabbit beta-globin (CAG) hybrid promoter instead of the classical CMV promoter to drive more efficient transgene expression in wider host cells. The specially designed cassette under CAG hybrid promoter contained two tandemly arrayed XcmI sites which were spaced by an additional EcoRV site. For direct cloning and expressing PCR-amplified ORFs, the T-vector was prepared by further digesting the EcoRV-linearized pCAGX with XcmI to produce T tails on both 3'-ends, which could efficiently minimize the non-recombinant background of T-vector and eliminate the necessity of selective marker genes such as LacZ that allowed blue/white screening. Various PCR fragments in length were prepared to verify the cloning efficiency by ligation with this vector, and GFP gene expression under control of the CAG hybrid promoter in different host cells was assayed by flow cytometry. The results indicated that this vector was higher efficient, especially suitable for cloning and expressing a number of interesting ORFs in parallel, and higher-level transgene expression in different mammalian cells was obtained than the reported vectors using the CMV promoter.

Published
2009
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