Search

Your search keyword '"R. I. Yakubovskaya"' showing total 2 results
Start Over Author "R. I. Yakubovskaya" Publisher springer science and business media llc
2 results on '"R. I. Yakubovskaya"'

1. [Untitled]

Author

A. I. Medvedev, R I Yakubovskaya, Vladimir S. Akatov, Leshchenko Vv, E. I. Lezhnev, Solov'eva Me, and N D Kreshchenko

Subjects
DNA damage, General Medicine, Biology, medicine.disease, Ascorbic acid, Molecular biology, General Biochemistry, Genetics and Molecular Biology, In vitro, Ehrlich ascites carcinoma, chemistry.chemical_compound, chemistry, Biochemistry, Carcinoma, medicine, Vitamin B12b, Incubation, DNA
Abstract

Incubation of Ehrlich ascites carcinoma and HEp-2 human epidermoid laryngeal carcinoma cells with hydroxycobalamin (vitamin B12b) and ascorbic acid induced generation and accumulation of double-stranded DNA fragments (23,000 b.p. and longer) in cells. The same vitamins alone in the same concentrations produced no such effects. DNA degradation in HEp-2 cells caused by long-term (4 h) incubation with 5-25 microM hydroxycobalamin and ascorbic acid (1:10-1:40 molar ratio) at 37 degrees C was comparable with that induced by gamma-irradiation in a dose of 150 Gy at 4 degrees C.

Published
2001

2. Isolation and purification of biopolymers by affinity chromatography. VI. Preparation and properties of an affinity adsorbent with a polysaccharide spacer for the purification of proteolytic enzymes

Author

V. Kh. Mitina, F. M. Shemyakin, B. A. Klyashchitskii, and R. I. Yakubovskaya

Subjects
chemistry.chemical_classification, Sorbent, Chromatography, Kunitz STI protease inhibitor, Proteolytic enzymes, virus diseases, food and beverages, Plant Science, General Chemistry, urologic and male genital diseases, Trypsin, Polysaccharide, female genital diseases and pregnancy complications, General Biochemistry, Genetics and Molecular Biology, carbohydrates (lipids), chemistry.chemical_compound, Adsorption, chemistry, Affinity chromatography, medicine, Cyanogen bromide, medicine.drug
Abstract

A new affinity sorbent has been synthesized — soybean trypsin inhibitor (STI)-amylopectin-hydrazidosuccinyl-Sepharose — and its properties have been studied in comparison with those of an analogous adsorbent without the spacer STI-Sepharose. The STI-amylopectin-hydrazidosuccinyl-Sepharose adsorbent has been used for the purification of trypsin from porcine pancreas and of callicrein from human blood plasma.

Published
1980

Catalog

Books, media, physical & digital resources
See catalog results