1. High expression of HMGB1 in children with refractory Mycoplasma pneumoniae pneumonia
- Author
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Yuqin Li, Zhengrong Chen, Gen Li, Chu Chu, Ying Ding, Weifang Zhou, and Xiaoli Lei
- Subjects
Male ,0301 basic medicine ,medicine.medical_specialty ,Adolescent ,medicine.medical_treatment ,030106 microbiology ,chemical and pharmacologic phenomena ,Inflammation ,lcsh:Infectious and parasitic diseases ,Pathogenesis ,03 medical and health sciences ,Medical microbiology ,Immune system ,Drug Resistance, Bacterial ,Pneumonia, Mycoplasma ,Lipid-associated membrane proteins (LAMPs) ,medicine ,Humans ,lcsh:RC109-216 ,Clinical significance ,Lymphocytes ,Treatment Failure ,HMGB1 Protein ,Child ,Cells, Cultured ,HMGB1 ,Lung ,business.industry ,Infant ,M. Pneumoniae pneumonia ,medicine.disease ,Mycoplasma pneumoniae ,Up-Regulation ,Community-Acquired Infections ,Pneumonia ,030104 developmental biology ,Infectious Diseases ,medicine.anatomical_structure ,Cytokine ,Child, Preschool ,Immunology ,Female ,medicine.symptom ,RMPP ,business ,Biomarkers ,Research Article - Abstract
Background Increasing numbers of refractory or severe, even fatal, cases of Mycoplasma pneumoniae infections have been reported in recent years. Excessive inflammatory responses play a vital role in the pathogenesis of refractory M. pneumoniae pneumonia (RMPP). HMGB1 is an actively secreted cytokine produced by macrophages and other inflammatory cells that participates in various infectious diseases. The present study aimed to explore the role and clinical significance of HMGB1 in children with RMPP and the potential mechanism of HMGB1 expression. Methods Four hundred and fifty-two children diagnosed with M. pneumoniae pneumonia, including 108 children with RMPP, were enrolled from January 2013 to December 2015 at the Children’s Hospital of Soochow University. HMGB1, TNF-α, and IL-6 in peripheral blood from RMPP and non-RMPP (NRMPP) cases were detected by real-time PCR and ELISA. Lipid-associated membrane proteins (LAMPs) were extracted from live M. pneumoniae and prepared at different concentrations for stimulation of THP-1 cells. After coculture with LAMPs, HMGB1, TNF-α, IL-6, RAGE, TLR2, and TLR4 in THP-1 cells were detected by real-time PCR. Results Occurrences of cough, fever, and abnormal lung signs were more frequent in RMPP cases compared with NRMPP cases (all p
- Published
- 2018
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