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Background Metal oxide nanoparticles (NPs) are becoming valuable due to their novel applications. The green synthesis of TiO2 NPs is more popular as a flexible and eco-friendly method compared to traditional chemical synthesis methods. TiO2 NPs are the most commonly used semiconductor in dye-sensitized solar cells (DSSCs). Results The biogenic TiO2 NPs were produced extracellularly by the marine halophilic bacterium Halomonas sp. RAM2. Response surface methodology (RSM) was used to optimize the biosynthesis process, resulting in a starting TiO2 concentration of 0.031 M and a pH of 5 for 92 min (⁓15 nm). TiO2 NPs were well-characterized after the calcination process at different temperatures of 500, 600, 700 and 800 °C. Anatase TiO2 NPs (calcined at 500 °C) with a smaller surface area and a wider bandgap were nominated for use in natural dye-sensitized solar cells (NDSSCs). The natural dye used as a photosensitizer is a mixture of three carotenoids extracted from the marine bacterium Kocuria sp. RAM1. NDSSCs were evaluated under standard illumination. After optimization of the counter electrode, NDSSCBio(10) (10 layers) demonstrated the highest photoelectric conversion efficiency (η) of 0.44%, which was almost as good as NDSSCP25 (0.55%). Conclusion The obtained results confirmed the successful green synthesis of TiO2 NPs and suggested a novel use in combination with bacterial carotenoids in DSSC fabrication, which represents an initial step for further efficiency enhancement studies.

Marine pigmented bacteria are a promising natural source of carotenoids. Kocuria sp. RAM1 was isolated from the Red Sea Bohadschia graeffei collected from Marsa Alam, Egypt, and used for carotenoids production. The extracted carotenoids were purified by thin-layer chromatography (TLC). The characteristic UV absorbance of the three purified fractions gave us an inkling of what the purified pigments were. The chemical structures were confirmed by nuclear magnetic resonance spectroscopy (NMR) and LC-ESI-QTOF-MS/MS. The three different red pigments were identified as two C50-carotenoids, namely bisanhydrobacterioruberin and trisanhydrobacterioruberin, in addition to 3,4,3ʹ,4ʹ-Tetrahydrospirilloxanthin (C42-carotenoids). Kocuria sp. RAM1 carotenoids were investigated for multiple activities, including antimicrobial, anti-inflammatory, antioxidant, anti-HSV-1, anticancer, antidiabetic and wound healing. These new observations suggest that Kocuria sp. RAM1 carotenoids can be used as a distinctive natural pigment with potent properties.