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Your search keyword '"Schaap, Nicolaas P. M."' showing total 6 results
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6 results on '"Schaap, Nicolaas P. M."'

1. Human CD34+-derived complete plasmacytoid and conventional dendritic cell vaccine effectively induces antigen-specific CD8+ T cell and NK cell responses in vitro and in vivo

Author

van Eck van der Sluijs, Jesper, primary, van Ens, Diede, additional, Brummelman, Jolanda, additional, Heister, Daan, additional, Sareen, Aastha, additional, Truijen, Lisa, additional, van Ingen Schenau, Dorette S., additional, Heemskerk, Mirjam H. M., additional, Griffioen, Marieke, additional, Kester, Michel G. D., additional, Schaap, Nicolaas P. M., additional, Jansen, Joop H., additional, van der Waart, Anniek B., additional, Dolstra, Harry, additional, and Hobo, Willemijn, additional

Published
2023
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2. Clinically applicable CD34+-derived blood dendritic cell subsets exhibit key subset-specific features and potently boost anti-tumor T and NK cell responses

Author

van Eck van der Sluijs, Jesper, van Ens, Diede, Thordardottir, Soley, Vodegel, Denise, Hermens, Inge, van der Waart, Anniek B., Falkenburg, J. H. Frederik, Kester, Michel G. D., de Rink, Iris, Heemskerk, Mirjam H. M., Borst, Jannie, Schaap, Nicolaas P. M., Jansen, Joop H., Xiao, Yanling, Dolstra, Harry, and Hobo, Willemijn

Subjects
CD34+ hematopoietic progenitor cells, Cancer Research, T-Lymphocytes, Cancer development and immune defence Radboud Institute for Molecular Life Sciences [Radboudumc 2], medicine.medical_treatment, Immunology, Cell, Cell Culture Techniques, T cells, CD34, Antigens, CD34, CD34(+) hematopoietic progenitor cells, NK cells, Biology, Lymphocyte Activation, Dendritic cells, 03 medical and health sciences, Cross-Priming, 0302 clinical medicine, Immunity, medicine, Humans, Immunology and Allergy, 030304 developmental biology, Antigen Presentation, 0303 health sciences, Vaccination, Dendritic cell, Immunotherapy, Hematopoietic Stem Cells, Acquired immune system, Clinical application, Killer Cells, Natural, Transplantation, medicine.anatomical_structure, Oncology, Cancer research, Original Article, Stem cell, 030215 immunology
Abstract

Allogeneic stem cell transplantation (alloSCT), following induction chemotherapy, can be curative for hemato-oncology patients due to powerful graft-versus-tumor immunity. However, disease recurrence remains the major cause of treatment failure, emphasizing the need for potent adjuvant immunotherapy. In this regard, dendritic cell (DC) vaccination is highly attractive, as DCs are the key orchestrators of innate and adaptive immunity. Natural DC subsets are postulated to be more powerful compared with monocyte-derived DCs, due to their unique functional properties and cross-talk capacity. Yet, obtaining sufficient numbers of natural DCs, particularly type 1 conventional DCs (cDC1s), is challenging due to low frequencies in human blood. We developed a clinically applicable culture protocol using donor-derived G-CSF mobilized CD34+ hematopoietic progenitor cells (HPCs) for simultaneous generation of high numbers of cDC1s, cDC2s and plasmacytoid DCs (pDCs). Transcriptomic analyses demonstrated that these ex vivo-generated DCs highly resemble their in vivo blood counterparts. In more detail, we demonstrated that the CD141+CLEG9A+ cDC1 subset exhibited key features of in vivo cDC1s, reflected by high expression of co-stimulatory molecules and release of IL-12p70 and TNF-α. Furthermore, cDC1s efficiently primed alloreactive T cells, potently cross-presented long-peptides and boosted expansion of minor histocompatibility antigen-experienced T cells. Moreover, they strongly enhanced NK cell activation, degranulation and anti-leukemic reactivity. Together, we developed a robust culture protocol to generate highly functional blood DC subsets for in vivo application as tailored adjuvant immunotherapy to boost innate and adaptive anti-tumor immunity in alloSCT patients.

Published
2021

3. Low relapse risk in poor risk AML after conditioning with 10-day decitabine, fludarabine and 2 Gray TBI prior to allogeneic hematopoietic cell transplantation

Author

Cruijsen, Marjan, primary, Hilberink, Jacobien R., additional, van der Velden, Walter J. F. M., additional, Jansen, Joop H., additional, Bär, Brigitte, additional, Schaap, Nicolaas P. M., additional, de Haan, Anton, additional, Mulder, André B., additional, de Groot, Marco R., additional, Baron, Frédéric, additional, Vellenga, Edo, additional, Blijlevens, Nicole N. M., additional, and Huls, Gerwin, additional

Published
2021
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4. Underdiagnosed veno-occlusive disease/sinusoidal obstruction syndrome (VOD/SOS) as a major cause of multi-organ failure in acute leukemia transplant patients: an analysis from the EBMT Acute Leukemia Working Party

Author

Bazarbachi, Abdul Hamid, primary, Al Hamed, Rama, additional, Labopin, Myriam, additional, Halaburda, Kazimierz, additional, Labussiere, Helene, additional, Bernasconi, Paolo, additional, Schroyens, Wilfried, additional, Gandemer, Virginie, additional, Schaap, Nicolaas P. M., additional, Loschi, Michael, additional, Jindra, Pavel, additional, Snowden, John, additional, Wu, Depei, additional, Guffroy, Blandine, additional, Rovira, Montserrat, additional, Chantepie, Sylvain P., additional, Poiré, Xavier, additional, Lopez-Corral, Lucia, additional, Nikolousis, Manos, additional, Pelosini, Matteo, additional, Ciceri, Fabio, additional, Baron, Frederic, additional, Bazarbachi, Ali, additional, Corbacioglu, Selim, additional, Savani, Bipin N., additional, Peric, Zinaida, additional, Nagler, Arnon, additional, Carreras, Enric, additional, and Mohty, Mohamad, additional

Published
2020
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5. PD-L1 siRNA-mediated silencing in acute myeloid leukemia enhances anti-leukemic T cell reactivity

Author

van Ens, Diede, primary, Mousset, Charlotte M., additional, Hutten, Tim J. A., additional, van der Waart, Anniek B., additional, Campillo-Davo, Diana, additional, van der Heijden, Sanne, additional, Vodegel, Denise, additional, Fredrix, Hanny, additional, Woestenenk, Rob, additional, Parga-Vidal, Loreto, additional, Jansen, Joop H., additional, Schaap, Nicolaas P. M., additional, Lion, Eva, additional, Dolstra, Harry, additional, and Hobo, Willemijn, additional

Published
2020
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