Glycerol Dialkyl Glycerol Tetraethers (GDGTs) serve as important tools for the quantitative reconstruction of paleoclimate and paleoecology in both continental and marine environments. Previous studies of GDGTs in the terrestrial environments focused primarily on the soils from the relatively warm-humid or cold-dry regions. However, it is still unclear how GDGTs respond to environmental variables in the cold-humid regions. Here, we collected soils along an altitudinal transect of Mountain (Mt.) Changbai, which has a typical cold-humid climate, to investigate the distribution of GDGTs and the response of GDGT-based proxies to changes in climate along the transect. The shift in the distribution of archaeal isoprenoidal GDGTs (isoGDGTs) revealed that the archaeal community varied significantly along the transect, which can affect the relationship between TEX86 and mean annual air temperature (MAT). In addition, the increased temperature seasonality at higher altitudes exerted a significant impact on TEX86. We proposed a global calibration of TEX86 for the growing season temperature reconstruction in the soil environments: T =85.19×TEX86− 46.30 ( R 2=0.84, p 0.55 are diagnostic for the cold-humid climate. Thus, the combination of MBT′5me and MBT′6me has the potential as a tool for the identification of different types of paleoclimate.