1. Fibroblast Growth Factor-2 Augments Recombinant Human Bone Morphogenetic Protein-2-Induced Osteoinductive Activity
- Author
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Akiko Sasaki, Eiji Tanaka, Mineo Watanabe, Takuro Hasegawa, Eizo Yamano, Diego A. Dalla-Bona, Yoshihiro Ishino, Theo M.G.J. van Eijden, Kazuo Tanne, and Functionele_Anatomie (OUD, ACTA)
- Subjects
Male ,medicine.medical_specialty ,Biomedical Engineering ,Bone Morphogenetic Protein 2 ,Bone morphogenetic protein ,Fibroblast growth factor ,Bone morphogenetic protein 2 ,law.invention ,Osteogenesis ,Transforming Growth Factor beta ,law ,Internal medicine ,medicine ,Animals ,Humans ,Rats, Wistar ,Fibroblast ,Bone Transplantation ,Dose-Response Relationship, Drug ,Skull Fractures ,Chemistry ,Drug Synergism ,medicine.disease ,Recombinant Proteins ,Rats ,Drug Combinations ,Endocrinology ,medicine.anatomical_structure ,Bone Morphogenetic Proteins ,Recombinant DNA ,Alkaline phosphatase ,Fibroblast Growth Factor 2 ,Parietal bone ,Calcification - Abstract
The osteoinductive activity induced by recombinant human BMP-2 (rhBMP-2) blunts proportionately as the recipient ages. In order to compensate for this bluntness administration of fibroblast growth factor-2 (FGF-2) has been considered. The aim of this study was to determine whether FGF-2 administration augments osteoinductive activity caused by rhBMP-2 and to evaluate the effect of aging on bone formation induced by coadministration of rhBMP-2 and FGF-2. Sixty-four Wistar strain male rats of 8-week-old (prepubertal) and 16-week-old (postpubertal) received bone defects bilaterally in the parietal bone and the defects were filled by a polylactic acid polyglycolic acid copolymer/gelatin sponge (PGS) impregnated with rhBMP-2 plus 0 ng, 25 ng, and 250 ng FGF-2 (n=10 in each). At 2 weeks after grafting, the new bone volume seemed to be larger in the rhBMP-2+FGF-2 groups than in the rhBMP-2 alone group. At 4 weeks, the new bone formation was linked to the adjacent original bone. In the prepubertal rats, all newly formed bone was similarly calcified. In the postpubertal rats, only the rhBMP-2+25 ng FGF-2 group showed this higher degree of calcification. At 2 weeks, alkaline phosphatase (ALP) activity in the rhBMP-2+25 ng FGF-2 group was significantly (p
- Published
- 2006
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