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Studies have reported negative effects of self-control demands on the service-oriented physical and mental well-being of employees. Based on the stressor-detachment model and conservation of resources theory, the present study examined how and when the interplay between leisure crafting and perceived supervisor recreational sports support can lead self-control demands employees to exhibit proactive vitality management. A total of 212 employees completed surveys at three time points over six months. The results indicated that leisure crafting mediated the relationship between employees’ self-control demands and proactive vitality management. Perceived supervisor recreational sports support was shown to strengthen the relationship between leisure crafting and proactive vitality management. Furthermore, we demonstrated a moderated mediation model in which self-control demands, under employees’ perceived supervisor recreational sports support, did not motivate employees to engage in additional leisure crafting; rather, these demands enhanced employees’ leisure resources, which in turn promoted their proactive vitality management.

The current study is based on the self-determination theory, which proposes that basic psychological needs fulfillment may account for the relationship between mindfulness and athletes’ well-being. Using a two-wave longitudinal design, in the current study, we recruited 104 college athletes (mean age 20.66 years) and facilitated mindfulness, basic psychological needs fulfillment, positive and negative affects, life satisfaction, and subjective vitality measurements. Multiple regressions during the interaction term revealed that Time 1 basic psychological needs fulfillment mediated the relationship between Time 1 mindfulness and Time 2 hedonic well-being (satisfaction with life, positive and negative affects) and Time 2 eudaemonic well-being (subjective vitality) in athletes. In addition, the mediational effect remained evenly controlled in the Time 1 well-being index. Our hypothesis was supported, and the results are discussed in terms of mindfulness and its application in sports.

HLJ1 (DNAJB4), a DNAJ/Hsp40 chaperone, has emerged as a novel prognostic marker in lung cancers; however, the molecular contribution and functionality in neoplastic diseases remain to be established. This study demonstrated that HLJ1 inhibits epithelial-mesenchymal transition in vitro and reduces lung cancer metastasis in vivo. Using shRNA silencing and ectopic expression of HLJ1, we found that HLJ1 not only suppresses catalytic activity of Src but also downregulates the formation of oncogenic complexes associated with the EGFR, FAK and STAT3 signaling pathways. A screen of specimens from HLJ1-knockout mice and lung cancer patients validated that HLJ1 expression is inversely correlated with Src activity. Mechanistically, HLJ1 protein directly bound to catalytic and protein-binding domains of Src through its amino acid Y172 and the P301/P304 motif. Following Src-induced HLJ1 phosphorylation at Y172, HLJ1-Src interaction was elevated, resulting in Src inhibition and malignancy suppression. Interestingly, both Src-binding regions also occurred in other DNAJB family members and contributed to anti-invasive activities of DNAJB proteins. We conclude that HLJ1 is an endogenous Src inhibitor that can suppress cancer metastasis through complex interacting mechanisms. This HLJ1-Src complex might provide a promising molecular model for developing new anticancer strategies.

The aim of this study was to validate the Taiwanese version of the Acceptance and Action Questionnaire-II (AAQ-II) using a population that consisted of college students and athletes. The authors conducted three independent studies to examine the psychometric properties of the AAQ-II. Study 1 examined the validity of the AAQ-II in a sample of undergraduate students. Study 2 examined the group invariance of the AAQ-II between undergraduate students and athletes. Study 3 examined nomological validity by testing the mediating role of experiential avoidance in the relationship between perceived autonomy support and well−/ill-being indicators. Overall, the results of the confirmatory factor analyses conducted in Study 1 demonstrated the existence of a unidimensional AAQ-II. AAQ-II was positively correlated with depression. The internal consistency of the scale was 0.81. In Study 2, factorial invariance of the AAQ-II was demonstrated across undergraduate students and adolescent athletes. Study 3 showed that perceived autonomy support was related to both depression and negative emotion through experiential avoidance. However, experiential avoidance did not mediate the relationship between perceived autonomy support and positive emotion and life satisfaction. In summary, this study provided preliminary evidence to support the validity and reliability of the AAQ-II and opened a new avenue for cross-cultural researchers.

Molecular diagnosis of biofilm-related genes (BRGs) in common bacteria that cause periprosthetic joint infections may provide crucial information for clinicians. In this study, several BRGs, including ica, fnbA, and fnbB, were rapidly detected (within 1 h) with a new integrated microfluidic system. Mannose-binding lectin (MBL)-coated magnetic beads were used to isolate these bacteria, and on-chip nucleic acid amplification (polymerase chain reaction, PCR) was then performed to detect BRGs. Both eukaryotic and prokaryotic MBLs were able to isolate common bacterial strains, regardless of their antibiotic resistance, and limits of detection were as low as 3 and 9 CFU for methicillin-resistant Staphylococcus aureus and Escherichia coli, respectively, when using a universal 16S rRNA PCR assay for bacterial identification. It is worth noting that the entire process including bacteria isolation by using MBL-coated beads for sample pre-treatment, on-chip PCR, and fluorescent signal detection could be completed on an integrated microfluidic system within 1 h. This is the first time that an integrated microfluidic system capable of detecting BRGs by using MBL as a universal capturing probe was reported. This integrated microfluidic system might therefore prove useful for monitoring profiles of BRGs and give clinicians more clues for their clinical judgments in the near future.

Periprosthetic joint infection (PJI) is one of the severe complications of prosthetic joint replacement. Delayed PJI diagnosis may anchor bacteria in periprosthetic tissues, and removal of the prosthesis might be inevitable. The diagnosis of PJI depends on the identification of microorganisms by standard microbiological cultures or more advanced molecular diagnostic methods for detection of bacterial genes. However, these methods are relatively time-consuming, labor-intensive and not human error-free. Moreover, it is challenging to distinguish live from dead bacteria by using DNA-based molecular diagnostics since bacterial DNA will be remained in the tissue even after the death of the bacteria. In this work, an integrated microfluidic system has been developed to perform the entire molecular diagnostic process for the PJI diagnosis in a single chip. We combined the loop-mediated isothermal amplification (LAMP) with ethidium monoazide (EMA) in an integrated microfluidic system to identify live bacteria with reasonable sensitivity and high specificity. All the diagnostic processes including bacteria isolation, cell lysis, DNA amplification and optical detection can be automatically performed on the integrated microfluidic system by using a compact custom-made control system. The integrated system can accommodate four primers complementary to six regions of the target genes and improve the detection limit by using LAMP. The limit of detection in this multiple EMA-LAMP assay could be as low as 5 fg/reaction (~1 CFU/reaction) when choosing an optimized primer set as we demonstrated in mecA gene detection. Thus, the developed system for PJI diagnosis has great potential to become a point-of-care device.

Background Long noncoding RNAs (lncRNAs) play crucial roles in tumorigenesis, and lncRNA taurine-upregulated gene 1 (TUG1) has been proven to be associated with several human cancers. However, the mechanisms of TUG1-involved regulation remain largely unknown. Methods We examined the expressions of TUG1 in a cohort of 89 patients with non-small cell lung cancer (NSCLC) to determine the association between TUG1 expression and clinical parameters. We used circular chromosome conformation capture (4C) coupled with next-generation sequencing to explore the genome regions that interact with TUG1 and the TUG1-mediated regulation. Results TUG1 was significantly downregulated, and the TUG1 downregulation correlated with sex (p = 0.006), smoking status (p = 0.016), and tumor differentiation grade (p = 0.001). Knockdown of TUG1 significantly promoted the proliferation of NSCLC cells. According to the bioinformatic analysis result of TUG1 4C sequencing data, 83 candidate genes and their interaction regions were identified. Among these candidate genes, CUGBP and Elav-like family member 1 (CELF1) are potential targets of TUG1 in-trans regulation. To confirm the interaction between TUG1 and CELF1, relative expressions of CELF1 were examined in TUG1 knockdown H520 cells; results showed that CELF1 was significantly upregulated in TUG1 knockdown H520 cells. RNA immunoprecipitation was then performed to examine whether TUG1 RNA was bound to PRC2, a TUG1-involved regulation mechanism reported in previous studies. The results demonstrated that TUG1 RNA was bound to enhancer of zeste protein 2/embryonic ectoderm development (EZH2/EED), which is essential for PRC2. Finally, our designed ChIP assay revealed that the EZH2/EED was bound to the promotor region of CELF1 within 992 bp upstream of the transcript start site. Conclusion TUG1 is downregulated in NSCLC. Using TUG1 4C sequencing and bioinformatic analysis, we found CELF1 to be a potential target of TUG1 RNA in in-trans regulation. Moreover, subsequent experiments showed that TUG1 RNA could bind to PRC2 in the promotor region of CELF1 and negatively regulate CELF1 expressions in H520 cells. Our results may facilitate developing new treatment modalities targeting TUG1/PRC2/CELF1 interactions in patients with NSCLC. Electronic supplementary material The online version of this article (doi:10.1186/s12885-016-2569-6) contains supplementary material, which is available to authorized users.

Many anodic aluminum oxide (AAO) templates were traditionally performed by potentiostatic anodization at 0---10 °C to inhibit the Joule's heat enhanced dissolution in aluminum oxide for ordered AAO configuration. In this article, the hybrid pulse anodization has been performed for offering the effective suppression of Joule's heat generation in high-aspect-ratio AAO formation at room temperature. The effect of pulse period, duty ratio, potential and electrolytic concentration on the evolution of pore characteristics was investigated using Taguchi method. The AAO morphology was captured by scanning electron microscope, and analyzed by gray-scale imaging in order to identify the pore size distribution and AAO thickness for Taguchi analysis. Short pulse-off period and low current density improved the uniformity of pore distribution. Moreover, high current density and intermediate pulse period/duty ratio can enhance the reaction rate and resulted in thick AAO. The evolution of AAO configuration and thickness were further correlated and discussed with pulse modulations and electrolytic concentrations.