1. Bacterial community characterization of water and intestine of the shrimp Litopenaeus stylirostris in a biofloc system
- Author
-
Denis Saulnier, Fabien Pierrat, Kevin Magré, Bénédicte Lorgeoux, David Piquemal, Emilie Cardona, Florian Noguier, Yannick Gueguen, Lagons, Ecosystèmes et Aquaculture Durable de Nouvelle Calédonie (LEADNC), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Ecosystèmes Insulaires Océaniens (UMR 241) (EIO), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de la Polynésie Française (UPF)-Institut Louis Malardé [Papeete] (ILM), Institut de Recherche pour le Développement (IRD), Interactions Hôtes-Pathogènes-Environnements (IHPE), Université de Perpignan Via Domitia (UPVD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), ACOBIOM, Unité de recherche Lagons, Ecosystèmes et Aquaculture Durable en Nouvelle Calédonie, Université de la Polynésie Française (UPF)-Institut Louis Malardé [Papeete] (ILM), Institut de Recherche pour le Développement (IRD)-Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), and Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Perpignan Via Domitia (UPVD)
- Subjects
Chlorophyll ,0301 basic medicine ,Aquaculture ,Water column ,RNA, Ribosomal, 16S ,Ammonium Compounds ,Culture environment ,SPECTROSCOPY ,CRYSTAL ,biology ,Viscosity ,Ecology ,[SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] ,Microbiota ,High-Throughput Nucleotide Sequencing ,MODULUS ,Biodiversity ,04 agricultural and veterinary sciences ,Hydrogen-Ion Concentration ,Creep ,Bacterial communities ,6. Clean water ,Shrimp ,Intestines ,Clear sea water ,Fresnoite ,CRYSTALLIZATION ,Water Microbiology ,Research Article ,Biofloc ,Microbiology (medical) ,Penaeidae ,Nitrogen Dioxide ,Environment ,Microbiology ,03 medical and health sciences ,BATISI2O7 ,Animals ,Seawater ,14. Life underwater ,Shellfish ,Detritus ,Bacteria ,Base Sequence ,business.industry ,Chlorophyll A ,FRESNOITE GLASS ,fungi ,Water ,biology.organism_classification ,Elasticity ,030104 developmental biology ,Microbial population biology ,SILICATE-GLASSES ,040102 fisheries ,TIO2 ,0401 agriculture, forestry, and fisheries ,Glass ,Water quality ,business - Abstract
International audience; Background: Biofloc technology (BFT), a rearing method with little or no water exchange, is gaining popularity in aquaculture. In the water column, such systems develop conglomerates of microbes, algae and protozoa, together with detritus and dead organic particles. The intensive microbial community presents in these systems can be used as a pond water quality treatment system, and the microbial protein can serve as a feed additive. The current problem with BFT is the difficulty of controlling its bacterial community composition for both optimal water quality and optimal shrimp health. The main objective of the present study was to investigate microbial diversity of samples obtained from different culture environments (Biofloc technology and clear seawater) as well as from the intestines of shrimp reared in both environments through high-throughput sequencing technology. Results: Analyses of the bacterial community identified in water from BFT and " clear seawater " (CW) systems (control) containing the shrimp Litopenaeus stylirostris revealed large differences in the frequency distribution of operational taxonomic units (OTUs). Four out of the five most dominant bacterial communities were different in both culture methods. Bacteria found in great abundance in BFT have two principal characteristics: the need for an organic substrate or nitrogen sources to grow and the capacity to attach to surfaces and co-aggregate. A correlation was found between bacteria groups and physicochemical and biological parameters measured in rearing tanks. Moreover, rearing-water bacterial communities influenced the microbiota of shrimp. Indeed, the biofloc environment modified the shrimp intestine microbiota, as the low level (27 %) of similarity between intestinal bacterial communities from the two treatments. Conclusion: This study provides the first information describing the complex biofloc microbial community, which can help to understand the environment-microbiota-host relationship in this rearing system.
- Published
- 2016