1. Gene expression regulation in the context of mouse interspecific mosaic genomes
- Author
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David L'Hôte, Ahmad Oulmouden, Reiner A. Veitia, Xavier Montagutelli, Daniel Vaiman, Catherine Serres, Unité de Génétique Moléculaire Animale (UGMA), Université de Limoges (UNILIM)-Institut National de la Recherche Agronomique (INRA), Vaiman, Anne, Institut Cochin (IC UM3 (UMR 8104 / U1016)), Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Limoges (UNILIM), Institut National de la Recherche Agronomique (INRA), Génétique des Mammifères, Institut Pasteur [Paris], This study was supported by grant 06-2005-MAMMIFERT-02 from the French Agence National pour la Recherche. David L'Hôte is funded by grants from the Institut National de la Santé et de la Recherche Médicale (INSERM) and Limoges University., We thank Isabelle Lanctin (Pasteur Institute) for her precious help in providing us with difficult to breed animals., Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), and Institut Pasteur [Paris] (IP)
- Subjects
Genetic Markers ,Male ,endocrine system ,INTERSPECIFIC HYBRIDS ,Mus spretus ,[SDV]Life Sciences [q-bio] ,Congenic ,Mice, Inbred Strains ,Context (language use) ,souris ,Biology ,Polymorphism, Single Nucleotide ,Genome ,Transcriptome ,03 medical and health sciences ,GENE REGULATION ,0302 clinical medicine ,Testis ,Animals ,RNA, Messenger ,Promoter Regions, Genetic ,Oligonucleotide Array Sequence Analysis ,030304 developmental biology ,Regulation of gene expression ,Genetics ,REGULATION DE GENES ,0303 health sciences ,Mosaicism ,Gene Expression Profiling ,Research ,Chromosome Mapping ,Sequence Analysis, DNA ,biology.organism_classification ,Gene expression profiling ,MICE ,Gene Expression Regulation ,hybride interspécifique ,Genetic marker ,Linear Models ,030217 neurology & neurosurgery - Abstract
The testis transcriptome of mouse strains containing homozygous segments of Mus spretus origin in a Mus musculus background was analyzed., Background Accumulating evidence points to the mosaic nature of the mouse genome. However, little is known about the way the introgressed segments are regulated within the context of the recipient genetic background. To address this question, we have screened the testis transcriptome of interspecific recombinant congenic mouse strains (IRCSs) containing segments of Mus spretus origin at a homozygous state in a Mus musculus background. Results Most genes (75%) were not transcriptionally modified either in the IRCSs or in the parent M. spretus mice, compared to M. musculus. The expression levels of most of the remaining transcripts were 'dictated' by either M. musculus transcription factors ('trans-driven'; 20%), or M. spretus cis-acting elements ('cis-driven'; 4%). Finally, 1% of transcripts were dysregulated following a cis-trans mismatch. We observed a higher sequence divergence between M. spretus and M. musculus promoters of strongly dysregulated genes than in promoters of similarly expressed genes. Conclusion Our study indicates that it is possible to classify the molecular events leading to expressional alterations when a homozygous graft of foreign genome segments is made in an interspecific host genome. The inadequacy of transcription factors of this host genome to recognize the foreign targets was clearly the major path leading to dysregulation.
- Published
- 2008
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