1. A paper-based competitive lateral flow immunoassay for multi β-agonist residues by using a single monoclonal antibody labelled with red fluorescent nanoparticles
- Author
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Ruiguo Wang, Xiaoou Su, Peilong Wang, and Wei Zhang
- Subjects
Agonist ,Paper ,Multi residues ,medicine.drug_class ,Swine ,Food Contamination ,02 engineering and technology ,Fluorescent nanoparticles ,Monoclonal antibody ,01 natural sciences ,Fluorophotometry ,Analytical Chemistry ,Pork tissue ,chemistry.chemical_compound ,Cimaterol ,Limit of Detection ,medicine ,Animals ,Detection limit ,Immunoassay ,Original Paper ,Chromatography ,medicine.diagnostic_test ,Chemistry ,Mabuterol ,Paper-based assay ,010401 analytical chemistry ,Antibodies, Monoclonal ,Adrenergic beta-Agonists ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Red Meat ,β-Agonist ,Clenbuterol ,Nanoparticles ,0210 nano-technology ,medicine.drug - Abstract
An ultrasensitive paper based lateral flow assay is described for rapid and simultaneous fluorometric detection of several β-agonists including clenbuterol and its chemical analogues (mabuterol, brombuterol, cimaterol, cimbuterol, bromchlorbuterol and banbuterol). A nonspecific monoclonal antibody (mAb) against clenbuterol and its analogues was prepared and employed in a competitive immunoassay where mAb conjugated to fluorescent nanoparticles and free β-agonists compete for the binding sites. This enables rapid screening for the 7 β-agonists in a single run that takes about 8 min. Detection limits for the seven β-agonists are
- Published
- 2018