Search

In Europe, spreading organic wastes to fertilize soils is an alternative commonly used instead of chemical fertilizers. Through their contributions of nutrients and organic matter, these wastes promote plant growth and thus agricultural production. However, these organic amendments can also contain mineral and organic pollutants requiring chemical and ecotoxicological analyses to guarantee their harmlessness on soil and its organisms during spreading. The purpose of this study was to assess the potential toxicity of three organic amendments from different sources (sewage sludge, dairy cow manure, dairy cow slurry) by performing chemical analyses and acute toxicity tests on three types of organism: earthworms, plants, soil microbial communities. Chemical analysis revealed a higher content of certain pharmaceuticals, polycyclic aromatic hydrocarbons and metals in sewage sludge in comparison with the two other types of organic wastes. The ecotoxicological assessment showed a dose-dependent effect on soil organisms for the three organic amendments with higher toxic effects during the exposure tests with a soil amended with dairy cow slurry. However, at realistic spreading doses (10 and 20 g kg -1 dry weight of organic amendments) on a representative exposed soil, organic amendment did not show any toxicity in the three organisms studied and had positive effects such as increased earthworm biomass, increased plant root growth and earthworm behavior showing attraction for organic amendment. On the contrary, exposure assays carried out on a limited substrate like sandy soil showed increased toxicity of organic amendments on plant germination and root growth. Overall, the ecotoxicological analysis revealed greater toxicity for soil organisms during the amendment of cow slurry, contrary to the chemical analysis which showed the potential high risk of spreading sewage sludge due to the presence of a higher quantity of pollutants. The analysis of the chemical composition and use of acute toxicity tests is the first essential step for assessing the ecotoxicological risk of spreading organic amendments on soil organisms. In addition to standard tests, the study suggests using a representative soil in acute toxicity tests to avoid overestimating the toxic effects of these organic amendments., (© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

Mediterranean climate characteristics and carbonate are key factors governing soil heavy-metal accumulation, and low organic matter (OM) content could limit the ability of microbial populations to cope with resulting stress. We studied the effects of metal contamination on a combination of biological parameters in soils having these characteristics. With this aim, soils were spiked with a mixture of cadmium, copper, lead, and zinc, at the two limit values proposed by current European legislation, and incubated for ≤12 months. Then we measured biochemical (phosphatase, urease, β-galactosidase, arylsulfatase, and dehydrogenase activities) and microbial (fungal and bacterial DNA concentration by quantitative polymerase chain reaction) parameters. All of the enzyme activities were strongly affected by metal contamination and showed the following inhibition sequence: phosphatase (30-64 %) < arylsulfatase (38-97 %) ≤ urease (1-100 %) ≤ β-galactosidase (30-100 %) < dehydrogenase (69-100 %). The high variability among soils was attributed to the different proportion of fine mineral fraction, OM, crystalline iron oxides, and divalent cations in soil solution. The decrease of fungal DNA concentration in metal-spiked soils was negligible, whereas the decrease of bacterial DNA was ~1-54 % at the lowest level and 2-69 % at the highest level of contamination. The lowest bacterial DNA decrease occurred in soils with the highest OM, clay, and carbonate contents. Finally, regarding the strong inhibition of the biological parameters measured and the alteration of the fungal/bacterial DNA ratio, we provide strong evidence that disturbance on the system, even within the limiting values of contamination proposed by the current European Directive, could alter key soil processes. These limiting values should be established according to soil characteristics and/or revised when contamination is produced by a mixture of heavy metals.