1. A biosensor based on Fe 3 O 4 @MXene-Au nanocomposites with high peroxidase-like activity for colorimetric and smartphone-based detection of glucose.
- Author
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Fei J, Yang W, Dai Y, Xu W, Fan H, Zheng Y, Zhang J, Zhu W, Hong J, and Zhou X
- Subjects
- Humans, Peroxidase, Glucose, Colorimetry, Glucose Oxidase, Smartphone, Hydrogen Peroxide, Sepharose, Peroxidases, Nanocomposites, Biosensing Techniques
- Abstract
A novel magnetic nanozyme Fe
3 O4 @MXene-Au nanocomposite, which possessed higher peroxidase-like activity than that of Fe3 O4 nanoparticles and Fe3 O4 @MXene nanocomposites, was developed. The outstanding magnetic properties of the nanozyme endowed it with the ability of simple and rapid separation, achieving great recyclability. Based on Fe3 O4 @MXene-Au nanocomposites and glucose oxidase (Glu Ox), a highly selective colorimetric biosensor for glucose detection was developed. Fe3 O4 @MXene-Au nanocomposites can catalyze H2 O2 produced from glucose catalyzed by glucose oxidase to ·OH and oxidize colorless 3,3',5,5'-tetramethylbenzidine (TMB) to blue oxidized TMB (oxTMB) with a significant absorbance at 652 nm. The linear range of glucose was 0-1.4 mM under optimal conditions, with a limit of detection (LOD) of 0.11 mM. Glucose in human whole blood was successfully detected with satisfactory recoveries. Furthermore, a facile agarose hydrogel detection platform was designed. With smartphone software, glucose detection can be realized by the agarose hydrogel platform, demonstrating the potential in on-site and visual detection of glucose., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)- Published
- 2023
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