1. FTIR spectro-imaging of collagen scaffold formation during glioma tumor development.
- Author
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Noreen R, Chien CC, Chen HH, Bobroff V, Moenner M, Javerzat S, Hwu Y, and Petibois C
- Subjects
- Animals, Brain Neoplasms chemistry, Brain Neoplasms genetics, Collagen genetics, Disease Progression, Extracellular Matrix chemistry, Gene Expression, Glioma chemistry, Glioma genetics, Image Interpretation, Computer-Assisted, Male, Principal Component Analysis, Protein Structure, Secondary, Rats, Brain Neoplasms diagnosis, Collagen chemistry, Glioma diagnosis, Spectroscopy, Fourier Transform Infrared
- Abstract
Evidence has recently emerged that solid and diffuse tumors produce a specific extracellular matrix (ECM) for division and diffusion, also developing a specific interface with microvasculature. This ECM is mainly composed of collagens and their scaffolding appears to drive tumor growth. Although collagens are not easily analyzable by UV-fluorescence means, FTIR imaging has appeared as a valuable tool to characterize collagen contents in tissues, specially the brain, where ECM is normally devoid of collagen proteins. Here, we used FTIR imaging to characterize collagen content changes in growing glioma tumors. We could determine that C6-derived solid tumors presented high content of triple helix after 8-11 days of growth (typical of collagen fibrils formation; 8/8 tumor samples; 91 % of total variance), and further turned to larger α-helix (days 12-15; 9/10 of tumors; 94 % of variance) and β-turns (day 18-21; 7/8 tumors; 97 % of variance) contents, which suggest the incorporation of non-fibrillar collagen types in ECM, a sign of more and more organized collagen scaffold along tumor progression. The growth of tumors was also associated to the level of collagen produced (P < 0.05). This study thus confirms that collagen scaffolding is a major event accompanying the angiogenic shift and faster tumor growth in solid glioma phenotypes.
- Published
- 2013
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