4 results on '"Wen, Xiao-Hui"'
Search Results
2. Phylogeographic analysis of porcine reproductive and respiratory syndrome virus 1 in Guangdong province, Southern China.
- Author
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Zhai SL, Lin T, Zhou X, Pei ZF, Wei ZZ, Zhang H, Wen XH, Chen QL, Lv DH, and Wei WK
- Subjects
- Amino Acid Sequence, Animals, China epidemiology, Farms, Molecular Typing, Open Reading Frames, Phylogeography, Porcine Reproductive and Respiratory Syndrome transmission, Porcine Reproductive and Respiratory Syndrome virology, Porcine respiratory and reproductive syndrome virus classification, Porcine respiratory and reproductive syndrome virus isolation & purification, Prevalence, Reverse Transcriptase Polymerase Chain Reaction, Sequence Alignment, Sequence Homology, Amino Acid, Swine, Genotype, Phylogeny, Porcine Reproductive and Respiratory Syndrome epidemiology, Porcine respiratory and reproductive syndrome virus genetics, RNA, Viral genetics, Viral Proteins genetics
- Abstract
Porcine reproductive and respiratory syndrome virus (PRRSV) is considered an important economic pathogen for the international swine industry. At present, both PRRSV-1 and PRRSV-2 have been confirmed to be co-circulating in China. However, there is little available information about the prevalence or distribution of PRRSV-1 in Guangdong province, southern China. In this study, we performed molecular detection of PRRSV-1 in 750 samples collected from 50 farms in 15 major pig farming regions in this province. After RT-PCR testing, 64% (32/50) of farms were confirmed as PRRSV-1-positive. Surprisingly, PRRSV-1 was circulating on at least one pig farm in all 15 regions; of the 750 samples, 186 samples (24.8%) were positive for PRRSV-1. Furthermore, 15 representative PRRSV-1 ORF5 sequences (606 bp) (n = 1 per region) were obtained from those PRRSV-1-positive regions. Sequence alignment analysis indicated that they shared 81.8% ~ 100% nucleotide and 81.2% ~ 100% amino acid similarity with each other. Although all current PRRSV-1 sequences were divided into pandemic subtype 1, most of them had unique glycoprotein-5 amino acid sequences that are significantly different from other known PRRSV-1 isolates. To conclude, the present findings revealed wide geographical distribution of PRRSV-1 in Guangdong province, southern China. This study further extends the epidemiological significance of PRRSV-1 in China.
- Published
- 2018
- Full Text
- View/download PDF
3. Molecular detection and genome characterization of porcine circovirus type 2 in rats captured on commercial swine farms.
- Author
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Zhai SL, Chen SN, Liu W, Li XP, Deng SF, Wen XH, Luo ML, Lv DH, Wei WK, and Chen RA
- Subjects
- Animals, China, Circoviridae Infections virology, Circovirus genetics, Cluster Analysis, DNA, Viral chemistry, DNA, Viral genetics, Genome, Viral, Phylogeny, Polymerase Chain Reaction, Sequence Analysis, DNA, Sequence Homology, Swine virology, Circoviridae Infections veterinary, Circovirus classification, Circovirus isolation & purification, Farms, Rats virology
- Abstract
Porcine circovirus type 2 (PCV2) is considered the major etiological pathogen of porcine circovirus-associated diseases (PCVADs) in pigs. Recently, PCV2 was also found in non-porcine animals such as cattle, rats, and mice. However, there was no record of PCV2 in rats in China. The goal of this study was to investigate whether PCV2 was present in rats (Rattus norvegicus, RN) on three swine farms, using molecular tools. PCR results showed that 30 of 95 (31.6 %) rat samples were positive for PCV2. Moreover, further genotype analysis suggested that 10 of 30 (33.3 %) were positive for PCV2a, 19 of 30 (63.3 %) were positive for PCV2b, and only one sample (1/30, 3.33 %) was co-infected by PCV2a and PCV2b. To determine the possible origin of PCV2, 60 serum samples were also collected from weaned pigs on those swine farms, and 23 out of 60 samples were positive for PCV2. In addition, two distinct RN-origin and two distinct porcine-origin PCV2 full-length nucleotide sequences were obtained from the farms. Sequence and phylogenetic analysis indicated that they had the highest nucleotide similarity and closest genetic relationships to each other. In this study, we report the infection and genome characterization of PCV2 in rats and compare RN-origin and porcine-origin PCV2 sequences obtained from the same pig farm, revealing possible cross-species transmission of PCV2.
- Published
- 2016
- Full Text
- View/download PDF
4. Emergence of reticuloendotheliosis virus in pigeons in Guangdong Province, Southern China.
- Author
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Zhai SL, Chen SN, Lin T, Wen XH, Wei WK, Lv DH, and Chen RA
- Subjects
- Animals, Bird Diseases epidemiology, Bird Diseases pathology, Chickens, China epidemiology, Ducks, Genome, Viral, Phylogeny, Poultry Diseases virology, Reticuloendotheliosis virus classification, Reticuloendotheliosis virus genetics, Reticuloendotheliosis virus physiology, Bird Diseases virology, Columbidae virology, Reticuloendotheliosis virus isolation & purification
- Abstract
Reticuloendotheliosis virus (REV), an important immunosuppressive pathogen, has many hosts, including chickens, ducks, geese, turkeys, and wild birds. Clinically, REV may lead to increased susceptibility to other pathogens, resulting in serious tissue damage (especially tumors) and the death of its host. In this study, we encountered a disease outbreak resulting in a large number of deaths of pigeons in Guangdong Province, Southern China. Histopathological analysis revealed apparent tumor-like lesions in multiple organs of pigeons. PCR assays for detection of tumor-associated pathogens (REV, avian leukosis virus, and Marek's disease virus) in poultry revealed the presence of REV sequences only. Moreover, fowlpox virus (FPV) with an insertion of REV long terminal repeat (LTR) sequences was also considered, but it was excluded using a specific PCR assay. To gain more genetic information, two full-length REV genome sequences were determined and found to have the highest nucleotide sequence similarity (99.9 %) and the closest genetic relationship to a vaccine strain (MD-2) and had a more distant genetic relationship (94.3 %) to a duck-origin strain (ATCC-VR775). To confirm the presence of REVs in pigeons, specific-pathogen-free (SPF) chickens and healthy pigeons were inoculated with microfiltered tumor tissue homogenates and were found to be susceptible to infection with REV. To our knowledge, this is the first report of REV in pigeons, and the data suggest that pigeons may be the natural host of REV.
- Published
- 2016
- Full Text
- View/download PDF
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