1. OH Radical-Induced Oxidation in Nucleosides and Nucleotides Unraveled by Tandem Mass Spectrometry and Infrared Multiple Photon Dissociation Spectroscopy
- Author
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Jiang, Yining, Clavaguéra, Carine, Indrajith, Suvasthika, Houée-Levin, Chantal, Berden, Giel, Oomens, Jos, Scuderi, Debora, Jiang, Yining, Clavaguéra, Carine, Indrajith, Suvasthika, Houée-Levin, Chantal, Berden, Giel, Oomens, Jos, and Scuderi, Debora
- Abstract
OH⋅-induced oxidation products of DNA nucleosides and nucleotides have been structurally characterized by collision-induced dissociation tandem mass spectrometry (CID-MS2) and Infrared Multiple Photon Dissociation (IRMPD) spectroscopy. CID-MS2 results have shown that the addition of one oxygen atom occurs on the nucleobase moiety. The gas-phase geometries of +16 mass increment products of 2’-deoxyadenosine (dA(O)H+), 2’-deoxyadenosine 5’-monophosphate (dAMP(O)H+), 2’-deoxycytidine (dC(O)H+), and 2’-deoxycytidine 5’-monophosphate (dCMP(O)H+) are extensively investigated by IRMPD spectroscopy and quantum-chemical calculations. We show that a carbonyl group is formed at the C8 position after oxidation of 2’-deoxyadenosine and its monophosphate derivative. For 2’-deoxycytidine and its monophosphate derivative, the oxygen atom is added to the C5 position to form a C−OH group. IRMPD spectroscopy has been employed for the first time to provide direct structural information on oxidative lesions in DNA model systems.
- Published
- 2023
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