1. Humanized Culture Medium for Clinical-Grade Generation of Erythroid Cells from Umbilical Cord Blood CD34+ Cells.
- Author
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Zamani, Majid, Yaghoubi, Yoda, Naimi, Adel, Hassanzadeh, Ali, Pourakbari, Ramin, Aghebati-Maleki, Leili, Motavalli, Roza, Aghlmandi, Afsoon, Mehdizadeh, Amir, Nazari, Mehdi, Yousefi, Mehdi, and Movassaghpour, Ali Akbar
- Subjects
BLOOD cells ,CORD blood ,SERUM-free culture media ,RED blood cell transfusion ,HEMATOPOIETIC stem cells ,BLOOD groups - Abstract
Purpose: Transfusion of red blood cells (RBCs) is a supportive and common treatment in surgical care, trauma, and anemia. However, in vivo production of RBC seems to be a suitable alternative for blood transfusions due to the limitation of blood resources, the possibility of disease transmission, immune reactions, and the presence of rare blood groups. Cell cultures require serum-free or culture media supplemented with highly expensive animal serum, which can transmit xenoviruses. Platelet lysate (PL) can be considered as a suitable alternative containing a high level of growth factors and a low production cost. Methods: Three-step culture media supplemented with PL or fetal bovine serum (FBS) were used for proliferation and differentiation of CD34+ umbilical cord blood stem cells to erythrocytes in co-culture with bone marrow mesenchymal stem cells (BM-MSCs). The cells were cultivated for 15 days and cell proliferation and expansion were assessed using cell counts at different days. Erythroid differentiation genes, CD71 and glycophorin A expression levels were evaluated. Results: Maximum hematopoietic stem cells (HSCs) proliferation was observed on day 15 in PL-containing medium (99±17×103-fold). Gene expression and surface markers showed higher differentiation of cells in PL-containing medium. Conclusion: The results of this study indicate that PL can enhance erythroid proliferation and differentiation of CD34+ HSCs. PL can also be used as a proper alternative for FBS in the culture medium and HSCs differentiation. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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