1. Cytokine profile of breast cell lines after different radiation doses.
- Author
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Bravatà V, Minafra L, Forte GI, Cammarata FP, Russo G, Di Maggio FM, Augello G, Lio D, and Gilardi MC
- Subjects
- Cell Line, Tumor, Cell Survival radiation effects, Culture Media, Conditioned metabolism, Dose-Response Relationship, Radiation, Humans, Phenotype, Radiation Tolerance, Breast Neoplasms pathology, Cytokines metabolism
- Abstract
Purpose: Ionizing radiation (IR) treatment activates inflammatory processes causing the release of a great amount of molecules able to affect the cell survival. The aim of this study was to analyze the cytokine signature of conditioned medium produced by non-tumorigenic mammary epithelial cell line MCF10A, as well as MCF7 and MDA-MB-231 breast cancer cell lines, after single high doses of IR in order to understand their role in high radiation response., Materials and Methods: We performed a cytokine profile of irradiated conditioned media of MCF10A, MCF7 and MDA-MB-231 cell lines treated with 9 or 23 Gy, by Luminex and ELISA analyses., Results: Overall, our results show that both 9 Gy and 23 Gy of IR induce the release within the first 72 h of cytokines and growth factors potentially able to influence the tumor outcome, with a dose-independent and cell-line dependent signature. Moreover, our results show that the cell-senescence phenomenon does not correlate with the amount of 'senescence-associated secretory phenotype' (SASP) molecules released in media. Thus, additional mechanisms are probably involved in this process., Conclusions: These data open the possibility to evaluate cytokine profile as useful marker in modulating the personalized radiotherapy in breast cancer care.
- Published
- 2017
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