1. Potential prebiotic substrates modulate composition, metabolism, virulence and inflammatory potential of an in vitro multi-species oral biofilm.
- Author
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Verspecht T, Van Holm W, Boon N, Bernaerts K, Daep CA, Masters JG, Zayed N, Quirynen M, and Teughels W
- Abstract
Background : Modulation of the commensal oral microbiota constitutes a promising preventive/therapeutic approach in oral healthcare. The use of prebiotics for maintaining/restoring the health-associated homeostasis of the oral microbiota has become an important research topic. Aims : This study hypothesised that in vitro 14-species oral biofilms can be modulated by (in)direct stimulation of beneficial/commensal bacteria with new potential prebiotic substrates tested at 1 M and 1%
(w/v) , resulting in more host-compatible biofilms with fewer pathogens, decreased virulence and less inflammatory potential. Methods : Established biofilms were repeatedly rinsed with N-acetyl-D-glucosamine, α-D-lactose, D-(+)-trehalose or D-(+)-raffinose at 1 M or 1%(w/v) . Biofilm composition, metabolic profile, virulence and inflammatory potential were eventually determined. Results : Repeated rinsing caused a shift towards a more health-associated microbiological composition, an altered metabolic profile, often downregulated virulence gene expression and decreased the inflammatory potential on oral keratinocytes. At 1 M, the substrates had pronounced effects on all biofilm aspects, whereas at 1%(w/v) they had a pronounced effect on virulence gene expression and a limited effect on inflammatory potential. Conclusion : Overall, this study identified four new potential prebiotic substrates that exhibit different modulatory effects at two different concentrations that cause in vitro multi-species oral biofilms to become more host-compatible., Competing Interests: Authors W.T, T.V, K.B., M.Q. (in the name of Katholieke Universiteit Leuven), N.B. (in the name of Universiteit Gent), C.D and J.M. (in the name of Colgate-Palmolive Company) are listed as inventors on a patent application filed by Colgate-Palmolive Company, Katholieke Universiteit Leuven and Universiteit Gent related to specific aspects of this manuscript. Dr. Daep and Dr. Masters are employed by Colgate-Palmolive, which partially sponsored this study. All other authors report no conflicts of interest related to this study., (© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.)- Published
- 2021
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