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The complete chloroplast genome sequence of Swertia souliei, an alpine annual herbaceous plant endemic to the Qinghai-Tibetan Plateau (QTP), is reported in this study. The plastome is 152,804 bp in length, with one large single-copy region of 83,195 bp, one small single-copy region of 18,096 bp, and two inverted repeat (IR) regions of 25,756 bp. It contains 130 genes, including 85 protein-coding, 8 ribosomal RNA, and 37 transfer RNA genes. Phylogenetic tree shows that this species is a sister to the clade containing S. leducii, S. verticillifolia, and S. mussotii. The complete chloroplast genome could provide significant insight for understanding the phylogenetic relationship of taxa within Gentianaceae.

Gentiana urnula, is an alpine medicinal endemic to the Qinghai-Tibetan Plateau. In this study, the complete chloroplast DNA sequence of G. urnula was assembled，and its genome is 149,064 bp in length, including a large single-copy region (LSC) of 81,158 bp and a small single-copy region (SSC) of 17,034 bp, which was separated by a pair of 25,436 bp inverted repeat regions (IRs). A total of 130 genes are detected, including 86 protein-coding genes, 37 tRNA genes, and 7 rRNA genes. The complete chloroplast genomes of G. urnula will help to study the genetic diversity and phylogenetic analysis of Gentianales.

Swertia cordata, a herbaceous plant, has been used in medicines as an alternative febrifuge and anthelmintic. In this study, the complete chloroplast DNA sequence of S. cordata was assembled. It is 153,429 bp in length, including a large single-copy region of 83,613 bp and a small single-copy region of 18,038 bp separated by a pair of inverted repeat regions of 25,889 bp each. A total of 129 genes are detected, including 84 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. The complete chloroplast genome of S. cordata will help to study the genetic diversity and phylogenetic analysis of Swertia.

Pomatosace filicula (Primulaceae) is an alpine biennial endemic to the Qinghai-Tibet Plateau, which is regarded as a kind of traditional Tibetan medicine. We assembled the whole chloroplast genome sequence of this endangered species based on Illumina pair-end sequencing data. The complete chloroplast genome was estimated to be 151,277 bp in length, comprising a large single copy region of 82,039 bp and a small single copy region of 23,848 bp, separated by a pair of 22,695 bp inverted repeat regions. The genome contained 121 genes, including 83 protein-coding genes (74 PCG species), 29 tRNA genes (24 tRNA species), and eight ribosomal RNA genes (four rRNA species). The overall AT content of P. filicula chloroplast genome is estimated to be 62.59%. This genetic resource will facilitate further conservation genetic studies of P. filicula based on designing new chloroplast DNA markers.

The whole chloroplast (cp) genome sequence of Musella lasiocarpa has been characterized from Illumina pair-end sequencing. The complete cp genome was 169,178 bp in length, containing a large single copy (LSC) region of 87,884 bp and a small single copy (SSC) region of 11,144 bp, which were separated by a pair of 35,075 bp inverted repeat (IR) regions. The genome contained 138 genes, including 88 protein-coding genes (87 PCG species), 37 tRNA genes (30 tRNA species), and eight ribosomal RNA genes (four rRNA species). The most of gene species occur as a single copy, while 23 gene species occur in double copies. The overall AT content of M. lasiocarpa cp genome is 63.3%, while the corresponding values of the LSC, SSC, and IR regions are 64.9, 69.2, and 60.3%, respectively. The cp genome sequence is similar to that of the genus Musa.