1. Essential oil from Lippia microphylla Cham. modulates nitric oxide pathway and calcium influx to exert a tocolytic effect in rat uterus.
- Author
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Silva MDCC, Souza ILL, Vasconcelos LHC, Ferreira PB, Araujo LCDC, Sampaio RS, Tavares JF, da Silva BA, and Cavalcante FA
- Subjects
- Animals, Female, NG-Nitroarginine Methyl Ester pharmacology, Nitric Oxide Synthase antagonists & inhibitors, Nitric Oxide Synthase metabolism, Oxytocin pharmacology, Potassium Channels metabolism, Potassium Chloride pharmacology, Rats, Uterine Contraction drug effects, Uterus metabolism, Calcium metabolism, Lippia chemistry, Nitric Oxide metabolism, Oils, Volatile pharmacology, Signal Transduction drug effects, Tocolytic Agents pharmacology, Uterus drug effects
- Abstract
The essential oil of Lippia microphylla (LM-OE) presents several pharmacological activities. This work evaluates the tocolytic effect of LM-OE on rats. LM-OE inhibited phasic contractions and relaxed tonic contractions on rat uterus. Considering that nitric oxide (NO) pathway regulates uterine contraction, LM-OE potency was attenuated in the presence of NO synthase (NOS) inhibitor and this reduction was reversed in the presence of a NOS substrate. Similarly, the relaxant potency of LM-OE was reduced in the presence of soluble guanylyl cyclase (sGC) and protein kinase G (PKG) inhibitors. LM-OE also demonstrates a positive modulation of large and small conductance calcium-activated, voltage-gated and adenosine triphosphate-sensitive potassium channels and inhibited curves to CaCl
2 as well as relaxed the uterus pre-contracted by S-(-)-Bay K8644, suggesting voltage-gated calcium channels type-1 (CaV 1) blockade. Thus, the tocolytic effect of LM-OE on rat involves positive modulation of NO/NOS/sGC/PKG/K+ -channels pathway and Ca2+ influx blockade through CaV 1.[Formula: see text].- Published
- 2021
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