1. Inhibition of IL-4 but not IFN-γ production by splenocytes of mice immunized with ovalbumin after oral administration of 5-hydroxymethylfurfural.
- Author
-
Khodaei, Hamed and Alizadeh, Mohammad
- Subjects
- *
INTERLEUKIN-4 , *OVALBUMINS , *INTERFERON gamma , *IMMUNIZATION , *ORAL medication , *HYDROXYMETHYLFURFURAL , *LABORATORY mice - Abstract
5-Hydroxymethylfurfural (5-HMF) is one of the most important products of maillard reaction. This study sought to elucidate the immunomodulatory effect of 5-HMF by evaluating IFN-γ and IL-4 production in BALB/c mice sensitized with ovalbumin (OVA). Four groups of BALB/c mice (n = 5 for each group) including control, vehicle and two 5-HMF (188 and 750 mg/kg bw) treatment groups were studied. Interleukin-4(IL-4) and interferon gamma (IFN-γ) levels were measured in culture supernatants of spleen cells using enzyme-linked immunosorbent assay. It was found that the IL-4 level was significantly suppressed in 5-HMF treatment groups compared with the vehicle group (P < .05). Moreover, the IFN-γ/IL-4 ratio was significantly increased in a dose-independent manner compared with the vehicle group (P < .05). Oral administration of 5-HMF inhibited IL-4 but not IFN-γ production by splenocytes of mice immunized with OVA. These changes may reflect that 5-HMF could be a novel therapeutic approach for prevention of (Th2) cytokine-dominant disease. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF