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Marine nitrogen (N) has been traced into terrestrial and freshwater food chains at petrel (Aves: Procellariiformes) colonies on the South Island of New Zealand, but the effects of N or other marine-derived nutrients on the productivity of mainland forests are unknown. Mean ring widths in matai (Prumnopitys taxifolia; n = 3) and rimu (Dacrydium cupressinum; n = 2) trees growing in a Westland petrel (Procellaria westlandica) colony were 0.9-1.4 mm yr-1, similar to ring widths reported elsewhere. However, series of much wider rings in cores showed that trees in a petrel colony can experience periods of accelerated growth. δ15N values of whole wood were, at 3.2-11.1‰, significantly enriched in comparison to other temperate forest trees. The highest δ15N values were at the cambial and pith ends of different cores, so isotopic fractionation during N remobilisation is unlikely to have been significant. The radial extent of 15N enrichment suggests that petrels have bred at the site since at least the mid 18th century. [ABSTRACT FROM AUTHOR]

In observational studies of treatment effects, matched samples have traditionally been constructed using two tools, namely close matches on one or two key covariates and close matches on the propensity score to stochastically balance large numbers of covariates. Here we propose a third tool, fine balance, obtained using the assignment algorithm in a new way. We use all three tools to construct a matched sample for an ongoing study of provider specialty in the treatment of ovarian cancer. Fine balance refers to exact balance of a nominal covariate, often one with many categories, but it does not require individually matched treated and control subjects for this variable. In the example the nominal variable has 72 = 9 x 8 categories formed from 9 possible years of diagnosis and 8 geographic locations or SEER sites. We obtain exact balance on the 72 categories and close individual matches on clinical stage, grade, year of diagnosis, and other variables using a distance. and stochastically balance a total of 61 covariates using a propensity score. Our approach finds an optimal match that minimizes a suitable distance subject to the constraint that fine balance is achieved. This is done by defining a special patterned distance matrix and passing it to a subroutine that solves the optimal assignment problem, which optimally pairs the rows and columns of a matrix using a polynomial time algorithm. In the example we used the function Proc Assign in SAS. A new theorem shows that with our patterned distance matrix, the assignment algorithm returns an optimal, finely balanced matched sample whenever one exists, and otherwise returns an infinite distance. indicating that no such matched sample exists. [ABSTRACT FROM AUTHOR]

The common "F test" for testing the equality of two population variances is very sensitive to the normality assumption, yet many introductory statistics books do not stress this sensitivity. Several alternative dispersion tests have been developed that do not assume normality, but these are rarely discussed other than in nonparametric statistics texts. Additionally, these tests generally require tables of critical values or software to calculate p values. We propose a simple graphical procedure, requiring no tables or software, that compares absolute deviations of one sample to another. With equal sample sizes, if one sample has the five largest absolute deviations, we conclude that its population has greater dispersion. We believe the simplicity of this test makes it a viable alternative to the often misleading F test and to other tests. [ABSTRACT FROM AUTHOR]

The objectives of the Environmental Services Department of Athens International Airport SA (AIA) are to monitor environmental conditions, ensure compliance with environmental regulations, communicate environmental information, and undertake initiatives to improve environmental conditions. Since 2000, the Environmental Services Department has been certified according to EN ISO 14001:1996. During the next audit it will seek certification according to EN ISO 14001: 2004. This case study introduces the main elements of the environmental management system (EMS) regarding design, development and implementation, and examines the benefits and challenges of its adoption. Certification has led to a number of operational,environmental and public relations benefits, such as effective environmental monitoring and proactive approach to environmental problems. At the same time,EMS adoption requires continuous internal and external reviews to ensure proper implementation. One of the main future challenges is to expand the EMS to other AIA departments as well as to encourage companies operating at the airport to adopt similar systems. [ABSTRACT FROM AUTHOR]

Proposes a method for testing dispersion effects in unreplicated fractional factorial designs based on geometric means of residual sample variances. Discussion of the dispersion-effect testing method when only a single dispersion effect is present; Simulations of the effect of multiple dispersions; Options in performing dispersion-effect testing.

Presents information on a study which learned about the experiences of students who participated in a science fair and Science Olympiad competitions. Methodology of the study; Results and discussion on the study; Conclusion.

Presents procedures for estimating interrater reliability using the SPSS for Windows 5.0 program. Interclass correlation; Repeated-measures ANOVA; Spearman-Brown Prophecy Formula.

Provides nationally representative information about proprietary schools, or private career schools, and their students. General attributes; Kinds of training offered; Enrollment; Amount and type of student financial aid received by students; Cost of school training.

Comments on the article by Jan Smedslund focusing on the pseudoempirical aspects of psychology. Role of empirical research in psychology; Views on the philosophy of science; Focus on science and language.

Palmitate attenuates insulin secretion and reduces the viability of insulinproducing cells. Previous studies identified the aberrant palmitoylation or mispalmitoylation of proteins as one mechanism by which palmitate causes β-cell damage. In this report, we identify a role for lysosomal protein degradation as a mechanism by which β cells defend themselves against excess palmitate. The cation-independent mannose 6-phosphate receptor (CI-MPR) is responsible for the trafficking of mannose 6-phosphate-tagged proteins to lysosomes via Golgi sorting and from extracellular locations through endocytosis. RINm5F cells, which are highly sensitive to palmitate, lack CI-MPR. The reconstitution of CI-MPR expression attenuates the induction of endoplasmic reticulum (ER) stress and the toxic effects of palmitate on RINm5F cell viability. INS832/13 cells express CI-MPR and are resistant to the palmitate-mediated loss of cell viability. The reduction of CI-MPR expression increases the sensitivity of INS832/13 cells to the toxic effects of palmitate treatment. The inhibition of lysosomal acid hydrolase activity by weak base treatment of islets under glucolipotoxic conditions causes islet degeneration that is prevented by the inhibition of protein palmitoylation. These findings indicate that defects in lysosomal function lead to the enhanced sensitivity of insulin-producing cells to palmitate and support a role for normal lysosomal function in the protection of β cells from excess palmitate. [ABSTRACT FROM AUTHOR]

A letter to the editor is presented acknowledging the readers interest on the article about the Count Five test published on the previous issue of the periodical.

Techniques are proposed for developing a monthly and weekly drought outlook and the drought outlook components are evaluated. A drought index, the surface water supply index (SWSI) was modified and used for the drought outlook. A water balance model (abcd) was successfully calibrated using a regional regression, including monthly and weekly factors, and was used to convert meteorology to hydrology. For the monthly drought outlook, an ensemble technique was applied, both with and without monthly industrial meteorology information (MIMI). For the weekly drought outlook, a deterministic forecasting technique was applied employing the Global Data Assimilation and Prediction System (GDAPS). The methodologies were applied to the Geum River basin in Korea. While only the weekly outlook using the GDAPS has sufficient forecasting capability to suggest it might be useful, the accuracy of the monthly drought outlook is expected to improve as the climate forecast accuracy increases. Editor Z.W. Kundzewicz; Associate editor D. Hughes Citation Kim, Y.-O., Lee, J.-K., and Palmer, R.N., 2012. A drought outlook study in Korea. Hydrological Sciences Journal, 57 (6), 1141–1153. [ABSTRACT FROM AUTHOR]

Evidence shows that those with non-communicable diseases (NCDs) are at higher risk for serious illness and mortality from COVID-19. In Kenya, about 50% of the COVID-19 patients who have died had an NCD. We sought to describe the challenges faced in accessing NCD medicines in Kenya during the pandemic, through a descriptive narrative informed by key stakeholders engaged in NCD service delivery and decision-making. Access to NCD medicines was affected at three levels, service delivery, health facility information systems and the medicines supply chain to health facilities. In response to these gaps, the Ministry of Health released clear directives and interim guidelines for continuity of NCD service delivery. However, implementation of guidelines was not apparent from conversations with county officials or from assessment of county services by the Ministry. Rather, heterogeneity was observed in counties' responsiveness to patient needs, where 5 out of 13 counties used mHealth technologies, while 5 had no established system to reach patients. COVID-19 amplified gaps that already existed in the system—particularly around lack of robust supply chains and sub-optimal health information systems. This descriptive paper will be useful to policy makers to provide a summary of the key challenges faced in accessing NCD medicines, identify gaps in medicines delivery, and make case for establishment of a more equitable health system to meet the needs of lower-income NCD patients. [ABSTRACT FROM AUTHOR]

TAF4b is a gonadal-enriched subunit of the TFIID complex that is required for mouse fertility. TAF4b-deficient male mice undergo a complex series of developmental defects that result in the inability to maintain long-term spermatogenesis. To decipher the transcriptional mechanisms upon which TAF4b functions in spermatogenesis, we used two-hybrid screening to identify a novel TAF4b-interacting transcriptional cofactor, ZFP628. Deletion analysis of both proteins reveals discrete and novel domains of ZFP628 and TAF4b protein that function to bridge their direct interaction in vitro. Moreover, co-immunoprecipitation of ZFP628 and TAF4b proteins in testis-derived protein extracts supports their endogenous association. Using CRISPR-Cas9, we disrupted the expression of ZFP628 in the mouse and uncovered a post-meiotic germ cell arrest at the round spermatid stage in the seminiferous tubules of the testis in ZFP628-deficient mice that results in male infertility. Coincident with round spermatid arrest, we find reduced mRNA expression of transition protein (Tnp1 and Tnp2) and protamine (Prm1 and Prm2) genes, which are critical for the specialized maturation of haploid male germ cells called spermiogenesis. These data delineate a novel association of two transcription factors, TAF4b and ZFP628, and identify ZFP628 as a novel transcriptional regulator of stage-specific spermiogenesis. [ABSTRACT FROM AUTHOR]

In a model, such as a logit regression model, we wish to compare the relative importance of two groups of predictors for various objectives. In the example that motivated this work, the model predicts patient outcomes during hospital stays, and we wish to measure the relative contribution of patient and hospital characteristics to the variation in outcomes among patients and among hospitals. This is done using the relative dispersion of patient and hospital contributions to the fitted outcomes. As is seen, this question is distinct from other common questions, including the quality of the overall fit, the degree to which the outcome is accurately predicted, the statistical significance of groups of predictors, and the correlations among and between groups of predictors. Relevant point estimates, confidence intervals, and hypothesis tests are developed. In the example, we examine three outcome measures and find that nearly all of the predictable variation in patient outcomes and most of the predictable variation in outcomes among hospitals reflects variation in patient characteristics rather than hospital characteristics; however, this is true in varying degrees for the three outcomes. [ABSTRACT FROM AUTHOR]

Lactobacillus plantarum 299v is a probiotic bacterium effective in treating symptoms of irritable bowel syndrome. A mouse model of irritable bowel syndrome (IBS) can be produced by rectal administration of 1% allyl isothiocyanate (oil of mustard) in 30% ethanol. This study examined the effect of L. plantarum 299v on colonic inflammation and motility in the oil of mustard model. L. plantarum 299v (1×109 cfu) was gavaged for up to 28 days, beginning either 7 days before (pretreatment) or 8 days after oil of mustard administration (post-treatment). Colonic interferon gamma (IFNγ) release as an assessment of the inflammatory response was measured at day 4 and day 20 following oil of mustard administration. Small intestinal transit was assessed via the dye-transit score technique at day 20. L. plantarum 299v reduced inflammation at both day 4 and day 20 and normalized intestinal transit rates in the oil of mustard murine IBS model. [ABSTRACT FROM AUTHOR]

We present a geographical analysis of the first 22 months of the Department of Defense's human immunodeficiency virus (HIV) screening program for military applicants. This analysis concerns four HIV epidemic loci in the United States: New York City; Miami, Florida: Houston, Texas; and San Francisco, California. The cartographic analysis, using a novel smoothing algorithm to stabilize county-based rates, highlights the diffusion of the HIV epidemic from areas with relatively high prevalence to areas with relatively low prevalence of antibody to HIV. While there are particularities specific to each region, the diffusion patterns are remarkably consistent: areas closer to an endemic focus experience a slight decline over time, while more distant areas increase. The diffusion process depicted in map sequences demonstrates expansion diffusion in all four areas, while some provide a clear indication of a mixture of expansion and hierarchical processes. A previous analysis of these data for a fifteen-month period had concluded there was no evidence of a geographic trend in the data. Those conclusions were based on all ages pooled and used multi-state geographic units, making it unlikely that trends in prevalence, which varied by proximity to HIV epicenters, would have been detected. In view of the temporal crossover between the endemic and nonendemic areas' prevalence rates observed in Florida, Texas and California, any spatial analysis of these data using statewide summary rates will obscure the spatial-temporal trends. These data provide compelling evidence for the existence of sub-state level epidemics growing spatially and temporally into areas some miles from the original epicenters. We hope these results will encourage other public health personnel to undertake detailed surveillance of the AIDS and HIV infection epidemics at a unit of analysis small enough to permit quantifying spatial diffusion of this epidemic. [ABSTRACT FROM AUTHOR]

The homeodomain (HD) transcription factors are a structurally conserved family of proteins that, through networks of interactions with other nuclear proteins, control patterns of gene expression during development. For example, the network interactions of the pituitary-specific HD protein Pit-1 control the development of anterior pituitary cells and regulate the expression of the hormone products in the adult cells. Inactivating mutations in Pit-1 disrupt these processes, giving rise to the syndrome of combined pituitary hormone deficiency. Pit-1 interacts with CCAAT/enhancer-binding protein alpha (C/EBPα) to regulate prolactin transcription. Here, we used the combination of biochemical analysis and live-cell microscopy to show that two different point mutations in Pit-1, which disrupted distinct activities, affected the dynamic interactions between Pit-1 and C/EBPα in different ways. The results showed that the first α-helix of the POU-S domain is critical for the assembly of Pit-1 with C/EBPα, and they showed that DNA-binding activity conferred by the HD is critical for the final intranuclear positioning of the metastable complex. This likely reflects more general mechanisms that govern cell-type-specific transcriptional control, and the results from the analysis of the point mutations could indicate an important link between the mislocalization of transcriptional complexes and disease processes. [ABSTRACT FROM AUTHOR]